Cargando…

High-Level Expression and Biochemical Properties of A Thermo-Alkaline Pectate Lyase From Bacillus sp. RN1 in Pichia pastoris With Potential in Ramie Degumming

Pectate lyases play an essential role in textiles, animal feed, and oil extraction industries. Pichia pastoris can be an ideal platform for pectate lyases production, and BspPel (a thermo-alkaline pectate lyase from Bacillus sp. RN1) was overexpressed by combined strategies, reaching 1859 U/mL in a...

Descripción completa

Detalles Bibliográficos
Autores principales:	Zheng, Xueyun, Zhang, Yimin, Liu, Xiaoxiao, Li, Cheng, Lin, Ying, Liang, Shuli
Formato:	Online Artículo Texto
Lenguaje:	English
Publicado:	Frontiers Media S.A. 2020
Materias:	Bioengineering and Biotechnology
Acceso en línea:	https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396651/ https://www.ncbi.nlm.nih.gov/pubmed/32850721 http://dx.doi.org/10.3389/fbioe.2020.00850

Ejemplares similares

The alkaline pectate lyase PEL168 of Bacillus subtilis heterologously expressed in Pichia pastoris is more stable and efficient for degumming ramie fiber
por: Zhang, Chengjie, et al.
Publicado: (2013)

Structure of an Alkaline Pectate Lyase and Rational Engineering with Improved Thermo-Alkaline Stability for Efficient Ramie Degumming
por: Zhou, Cheng, et al.
Publicado: (2022)

Improvement on Thermostability of Pectate Lyase and Its Potential Application to Ramie Degumming
por: Xu, Huan, et al.
Publicado: (2022)

Improvement of optimum pH and specific activity of pectate lyase from Bacillus RN.1 using loop replacement
por: Li, Piwu, et al.
Publicado: (2023)

Improving the Catalytic Performance of Pectate Lyase Through Pectate Lyase/Cu(3)(PO(4))(2) Hybrid Nanoflowers as an Immobilized Enzyme
por: Wu, Pan, et al.
Publicado: (2020)

An Effective Degumming Enzyme from Bacillus sp. Y1 and Synergistic Action of Hydrogen Peroxide and Protease on Enzymatic Degumming of Ramie Fibers
por: Guo, Fenfen, et al.
Publicado: (2013)

Fhl1p protein, a positive transcription factor in Pichia pastoris, enhances the expression of recombinant proteins
por: Zheng, Xueyun, et al.
Publicado: (2019)

Combined strategies for improving expression of Citrobacter amalonaticus phytase in Pichia pastoris
por: Li, Cheng, et al.
Publicado: (2015)

Green Process: Improved Semi-Continuous Fermentation of Pichia pastoris Based on the Principle of Vitality Cell Separation
por: Wang, Denggang, et al.
Publicado: (2021)

Optimization of bio-chemical degumming of Ramie fiber for improved strength & luster
por: Ray Chaudhuri, Shaon, et al.
Publicado: (2020)

Expression and Characterization of an Alginate Lyase and Its Thermostable Mutant in Pichia pastoris
por: Yang, Suxiao, et al.
Publicado: (2020)

Cell Surface Display of Thermomyces lanuginosus Lipase in Pichia pastoris
por: Yang, Jiaming, et al.
Publicado: (2020)

Artificial Transcription Factors for Tuneable Gene Expression in Pichia pastoris
por: Naseri, Gita, et al.
Publicado: (2021)

Polycistronic Expression System for Pichia pastoris Composed of Chitino- and Chitosanolytic Enzymes
por: Kaczmarek, Michal B., et al.
Publicado: (2021)

Rational design and structure-based engineering of alkaline pectate lyase from Paenibacillus sp. 0602 to improve thermostability
por: Zhou, Zhanping, et al.
Publicado: (2021)

Recycling of a selectable marker with a self-excisable plasmid in Pichia pastoris
por: Li, Cheng, et al.
Publicado: (2017)

Establishing a Eukaryotic Pichia pastoris Cell-Free Protein Synthesis System
por: Zhang, Lingkai, et al.
Publicado: (2020)

A Combinational Strategy for Effective Heterologous Production of Functional Human Lysozyme in Pichia pastoris
por: He, Huahua, et al.
Publicado: (2020)

Improved Production of Xylanase in Pichia pastoris and Its Application in Xylose Production From Xylan
por: Miao, Ting, et al.
Publicado: (2021)

Continuous Cultivation as a Tool Toward the Rational Bioprocess Development With Pichia Pastoris Cell Factory
por: Nieto-Taype, Miguel Angel, et al.
Publicado: (2020)

Combining Metabolic Engineering and Multiplexed Screening Methods for 3-Hydroxypropionic Acid Production in Pichia pastoris
por: Fina, Albert, et al.
Publicado: (2022)

Pectate Lyase Pollen Allergens: Sensitization Profiles and Cross-Reactivity Pattern
por: Pichler, Ulrike, et al.
Publicado: (2015)

Fed-Batch Production of Saccharomyces cerevisiae L-Asparaginase II by Recombinant Pichia pastoris MUT(s) Strain
por: Rodrigues, David, et al.
Publicado: (2019)

Synthesis and Assembly of Hepatitis B Virus-Like Particles in a Pichia pastoris Cell-Free System
por: Spice, Alex J., et al.
Publicado: (2020)

Corrigendum: Combining metabolic engineering and multiplexed screening methods for 3-hydroxypropionic acid production in Pichia pastoris
por: Fina, Albert, et al.
Publicado: (2022)

High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation
por: Lu, Yihong, et al.
Publicado: (2016)

Characterization of an Alkaline Alginate Lyase with pH-Stable and Thermo-Tolerance Property
por: Wang, Yanan, et al.
Publicado: (2019)

In Silico Characterization of Pectate Lyase Protein Sequences from Different Source Organisms
por: Dubey, Amit Kumar, et al.
Publicado: (2010)

Abundance and Genetic Diversity of Microbial Polygalacturonase and Pectate Lyase in the Sheep Rumen Ecosystem
por: Yuan, Peng, et al.
Publicado: (2012)

Innovative Bioprocess Strategies Combining Physiological Control and Strain Engineering of Pichia pastoris to Improve Recombinant Protein Production
por: Gasset, Arnau, et al.
Publicado: (2022)

Corrigendum: High-level expression of improved thermo-stable alkaline xylanase variant in Pichia Pastoris through codon optimization, multiple gene insertion and high-density fermentation
por: Lu, Yihong, et al.
Publicado: (2017)

Improved production of recombinant β-mannanase (TaMan5) in Pichia pastoris and its synergistic degradation of lignocellulosic biomass
por: Zheng, Fengzhen, et al.
Publicado: (2023)

Characteristics of Polygalacturonate Lyase C from Bacillus subtilis 7-3-3 and Its Synergistic Action with PelA in Enzymatic Degumming
por: Zou, Mouyong, et al.
Publicado: (2013)

Extracellular expression of alkaline phytase in Pichia pastoris: Influence of signal peptides, promoters and growth medium
por: Yang, Mimi, et al.
Publicado: (2015)

Exchange of endogenous and heterogeneous yeast terminators in Pichia pastoris to tune mRNA stability and gene expression
por: Ito, Yoichiro, et al.
Publicado: (2020)

Production of Pectate Lyase by Penicillium viridicatum RFC3 in Solid-State and Submerged Fermentation
por: Ferreira, Viviani, et al.
Publicado: (2010)

A Verticillium dahliae Pectate Lyase Induces Plant Immune Responses and Contributes to Virulence
por: Yang, Yuankun, et al.
Publicado: (2018)

Rice RBH1 Encoding A Pectate Lyase is Critical for Apical Panicle Development
por: He, Dong, et al.
Publicado: (2021)

A Meloidogyne graminicola Pectate Lyase Is Involved in Virulence and Activation of Host Defense Responses
por: Chen, Jiansong, et al.
Publicado: (2021)

A Phloem-Expressed PECTATE LYASE-LIKE Gene Promotes Cambium and Xylem Development
por: Bush, Max, et al.
Publicado: (2022)

Cannot write session to /tmp/vufind_sessions/sess_0sp0qrnti6s949nuoi8oe9nu4p