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Co-assembled Supramolecular Nanofibers With Tunable Surface Properties for Efficient Vaccine Delivery
The utilization of nanotechnology to deliver vaccines and modulate immunity has shown great potential in cancer therapy. Peptide-based supramolecular hydrogels as novel vaccine adjuvants have been found to effectively improve the immune response and tumor curative effect. In this study, we designed...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Frontiers Media S.A.
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396696/ https://www.ncbi.nlm.nih.gov/pubmed/32850613 http://dx.doi.org/10.3389/fchem.2020.00500 |
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author | Wang, Zhongyan Ren, Chunhua Shang, Yuna Yang, Cuihong Guo, Qingxiang Chu, Liping Liu, Jianfeng |
author_facet | Wang, Zhongyan Ren, Chunhua Shang, Yuna Yang, Cuihong Guo, Qingxiang Chu, Liping Liu, Jianfeng |
author_sort | Wang, Zhongyan |
collection | PubMed |
description | The utilization of nanotechnology to deliver vaccines and modulate immunity has shown great potential in cancer therapy. Peptide-based supramolecular hydrogels as novel vaccine adjuvants have been found to effectively improve the immune response and tumor curative effect. In this study, we designed a set of reduction-responsive self-assembled peptide precursors (Fbp-G(D)F(D)F(D)Y(D)(E, S, or K)-ss-ERGD), which can be reduced by glutathione (GSH) into Fbp-G(D)F(D)F(D)Y(D)(E, S or K)-SH for forming of hydrogel with different surface properties (E-gel, S-gel, and K-gel, respectively). Using the same method, co-assembled hydrogel vaccines (E-vac, S-vac, and K-vac, respectively) can also be prepared by mixing different precursors with antigens before GSH reduction. Through TEM observation of the nanostructure, we found that all the co-assembled hydrogels, especially K-vac, possessed much denser and more unified nanofiber networks as compared with antigen-free hydrogels, which were very suitable for antigen storage and vaccine delivery. Although the three peptides adopted similar β-sheet secondary structures, the mechanical properties of their resulted co-assembled hydrogel vaccines were obviously different. Compared to E-vac, S-vac had a much weaker mechanical property, while K-vac had a much higher. In vivo experiments, co-assembled hydrogel vaccines, especially K-vac, also promoted antibody production and anti-tumor immune responses more significantly than the other two vaccines. Our results demonstrated that co-assembled hydrogels formed by peptides and antigens co-assembly could act as effective vaccine delivery systems for boosting antibody production, and different immune effects can be acquired by tuning the surface properties of the involved self-assembling peptides. |
format | Online Article Text |
id | pubmed-7396696 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-73966962020-08-25 Co-assembled Supramolecular Nanofibers With Tunable Surface Properties for Efficient Vaccine Delivery Wang, Zhongyan Ren, Chunhua Shang, Yuna Yang, Cuihong Guo, Qingxiang Chu, Liping Liu, Jianfeng Front Chem Chemistry The utilization of nanotechnology to deliver vaccines and modulate immunity has shown great potential in cancer therapy. Peptide-based supramolecular hydrogels as novel vaccine adjuvants have been found to effectively improve the immune response and tumor curative effect. In this study, we designed a set of reduction-responsive self-assembled peptide precursors (Fbp-G(D)F(D)F(D)Y(D)(E, S, or K)-ss-ERGD), which can be reduced by glutathione (GSH) into Fbp-G(D)F(D)F(D)Y(D)(E, S or K)-SH for forming of hydrogel with different surface properties (E-gel, S-gel, and K-gel, respectively). Using the same method, co-assembled hydrogel vaccines (E-vac, S-vac, and K-vac, respectively) can also be prepared by mixing different precursors with antigens before GSH reduction. Through TEM observation of the nanostructure, we found that all the co-assembled hydrogels, especially K-vac, possessed much denser and more unified nanofiber networks as compared with antigen-free hydrogels, which were very suitable for antigen storage and vaccine delivery. Although the three peptides adopted similar β-sheet secondary structures, the mechanical properties of their resulted co-assembled hydrogel vaccines were obviously different. Compared to E-vac, S-vac had a much weaker mechanical property, while K-vac had a much higher. In vivo experiments, co-assembled hydrogel vaccines, especially K-vac, also promoted antibody production and anti-tumor immune responses more significantly than the other two vaccines. Our results demonstrated that co-assembled hydrogels formed by peptides and antigens co-assembly could act as effective vaccine delivery systems for boosting antibody production, and different immune effects can be acquired by tuning the surface properties of the involved self-assembling peptides. Frontiers Media S.A. 2020-07-21 /pmc/articles/PMC7396696/ /pubmed/32850613 http://dx.doi.org/10.3389/fchem.2020.00500 Text en Copyright © 2020 Wang, Ren, Shang, Yang, Guo, Chu and Liu. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Chemistry Wang, Zhongyan Ren, Chunhua Shang, Yuna Yang, Cuihong Guo, Qingxiang Chu, Liping Liu, Jianfeng Co-assembled Supramolecular Nanofibers With Tunable Surface Properties for Efficient Vaccine Delivery |
title | Co-assembled Supramolecular Nanofibers With Tunable Surface Properties for Efficient Vaccine Delivery |
title_full | Co-assembled Supramolecular Nanofibers With Tunable Surface Properties for Efficient Vaccine Delivery |
title_fullStr | Co-assembled Supramolecular Nanofibers With Tunable Surface Properties for Efficient Vaccine Delivery |
title_full_unstemmed | Co-assembled Supramolecular Nanofibers With Tunable Surface Properties for Efficient Vaccine Delivery |
title_short | Co-assembled Supramolecular Nanofibers With Tunable Surface Properties for Efficient Vaccine Delivery |
title_sort | co-assembled supramolecular nanofibers with tunable surface properties for efficient vaccine delivery |
topic | Chemistry |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396696/ https://www.ncbi.nlm.nih.gov/pubmed/32850613 http://dx.doi.org/10.3389/fchem.2020.00500 |
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