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Optimized flow cytometric protocol for the detection of functional subsets of low frequency antigen-specific CD4(+) and CD8(+) T cells
Detection of low-frequency cells using flow cytometry is challenging, as the sensitivity of the analysis is dependent on the signal-to-noise ratio, and a cell frequency of 1 in 10,000 cells is accepted as the lower limit of detection for standard flow cytometry. A solution to this problem is to pre-...
| Autores principales: | , , , , , |
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| Formato: | Online Artículo Texto |
| Lenguaje: | English |
| Publicado: |
Elsevier
2020
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| Materias: | |
| Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396823/ https://www.ncbi.nlm.nih.gov/pubmed/32775228 http://dx.doi.org/10.1016/j.mex.2020.101005 |