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Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo

Currently, most biological research relies on conventional experimental techniques that allow only static analyses at certain time points in vitro or ex vivo. However, if one could visualize cellular dynamics in living organisms, that would provide a unique opportunity to study key biological phenom...

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Autores principales: Choo, Yeon Woong, Jeong, Juhee, Jung, Keehoon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Biochemistry and Molecular Biology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396917/
https://www.ncbi.nlm.nih.gov/pubmed/32475382
http://dx.doi.org/10.5483/BMBRep.2020.53.7.069
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author Choo, Yeon Woong
Jeong, Juhee
Jung, Keehoon
author_facet Choo, Yeon Woong
Jeong, Juhee
Jung, Keehoon
author_sort Choo, Yeon Woong
collection PubMed
description Currently, most biological research relies on conventional experimental techniques that allow only static analyses at certain time points in vitro or ex vivo. However, if one could visualize cellular dynamics in living organisms, that would provide a unique opportunity to study key biological phenomena in vivo. Intravital microscopy (IVM) encompasses diverse optical systems for direct viewing of objects, including biological structures and individual cells in live animals. With the current development of devices and techniques, IVM addresses important questions in various fields of biological and biomedical sciences. In this mini-review, we provide a general introduction to IVM and examples of recent applications in the field of immunology, oncology, and vascular biology. We also introduce an advanced type of IVM, dubbed real-time IVM, equipped with video-rate resonant scanning. Since the real-time IVM can render cellular dynamics with high temporal resolution in vivo, it allows visualization and analysis of rapid biological processes.
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spelling pubmed-73969172020-08-10 Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo Choo, Yeon Woong Jeong, Juhee Jung, Keehoon BMB Rep Invited Mini Review Currently, most biological research relies on conventional experimental techniques that allow only static analyses at certain time points in vitro or ex vivo. However, if one could visualize cellular dynamics in living organisms, that would provide a unique opportunity to study key biological phenomena in vivo. Intravital microscopy (IVM) encompasses diverse optical systems for direct viewing of objects, including biological structures and individual cells in live animals. With the current development of devices and techniques, IVM addresses important questions in various fields of biological and biomedical sciences. In this mini-review, we provide a general introduction to IVM and examples of recent applications in the field of immunology, oncology, and vascular biology. We also introduce an advanced type of IVM, dubbed real-time IVM, equipped with video-rate resonant scanning. Since the real-time IVM can render cellular dynamics with high temporal resolution in vivo, it allows visualization and analysis of rapid biological processes. Korean Society for Biochemistry and Molecular Biology 2020-07-31 2020-07-31 /pmc/articles/PMC7396917/ /pubmed/32475382 http://dx.doi.org/10.5483/BMBRep.2020.53.7.069 Text en Copyright © 2020 by the The Korean Society for Biochemistry and Molecular Biology This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (http://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Invited Mini Review
Choo, Yeon Woong
Jeong, Juhee
Jung, Keehoon
Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo
title Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo
title_full Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo
title_fullStr Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo
title_full_unstemmed Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo
title_short Recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo
title_sort recent advances in intravital microscopy for investigation of dynamic cellular behavior in vivo
topic Invited Mini Review
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7396917/
https://www.ncbi.nlm.nih.gov/pubmed/32475382
http://dx.doi.org/10.5483/BMBRep.2020.53.7.069
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