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Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits

Semen changes the gene expression in endometrial and oviductal tissues modulating important processes for reproduction. We tested the hypothesis that mating and/or sperm-free seminal plasma deposition in the reproductive tract affect the expression of genes associated with sperm-lining epithelium in...

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Autores principales: Gardela, Jaume, Jauregi-Miguel, Amaia, Martinez, Cristina A., Rodriguez-Martinez, Heriberto, Lopez-Bejar, Manel, Alvarez-Rodriguez, Manuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397043/
https://www.ncbi.nlm.nih.gov/pubmed/32645906
http://dx.doi.org/10.3390/genes11070758
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author Gardela, Jaume
Jauregi-Miguel, Amaia
Martinez, Cristina A.
Rodriguez-Martinez, Heriberto
Lopez-Bejar, Manel
Alvarez-Rodriguez, Manuel
author_facet Gardela, Jaume
Jauregi-Miguel, Amaia
Martinez, Cristina A.
Rodriguez-Martinez, Heriberto
Lopez-Bejar, Manel
Alvarez-Rodriguez, Manuel
author_sort Gardela, Jaume
collection PubMed
description Semen changes the gene expression in endometrial and oviductal tissues modulating important processes for reproduction. We tested the hypothesis that mating and/or sperm-free seminal plasma deposition in the reproductive tract affect the expression of genes associated with sperm-lining epithelium interactions, ovulation, and pre-implantation effects (nerve growth factor, NGF; α/β hydrolase domain-containing protein 2, ABHD2; C-terminal tensin-like protein, CTEN or TNS4; and versican, VCAN) in the period 10–72 h post-mating. In Experiment 1, does (n = 9) were treated with gonadotropin-releasing hormone (GnRH) (control), GnRH-stimulated, and vaginally infused with sperm-free seminal plasma (SP-AI), or GnRH-stimulated and naturally mated (NM). In Experiment 2, does (n = 15) were GnRH-stimulated and naturally mated. Samples were retrieved from the internal reproductive tracts (cervix-to-infundibulum) 20 h post-treatment (Experiment 1) or sequentially collected at 10, 24, 36, 68, or 72 h post-mating (Experiment 2, 3 does/period). All samples were processed for gene expression analysis by quantitative PCR. Data showed an upregulation of endometrial CTEN and NGF by NM, but not by SP-AI. The findings suggest that the NGF gene affects the reproductive tract of the doe during ovulation and beyond, influencing the maternal environment during early embryonic development.
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spelling pubmed-73970432020-08-05 Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits Gardela, Jaume Jauregi-Miguel, Amaia Martinez, Cristina A. Rodriguez-Martinez, Heriberto Lopez-Bejar, Manel Alvarez-Rodriguez, Manuel Genes (Basel) Article Semen changes the gene expression in endometrial and oviductal tissues modulating important processes for reproduction. We tested the hypothesis that mating and/or sperm-free seminal plasma deposition in the reproductive tract affect the expression of genes associated with sperm-lining epithelium interactions, ovulation, and pre-implantation effects (nerve growth factor, NGF; α/β hydrolase domain-containing protein 2, ABHD2; C-terminal tensin-like protein, CTEN or TNS4; and versican, VCAN) in the period 10–72 h post-mating. In Experiment 1, does (n = 9) were treated with gonadotropin-releasing hormone (GnRH) (control), GnRH-stimulated, and vaginally infused with sperm-free seminal plasma (SP-AI), or GnRH-stimulated and naturally mated (NM). In Experiment 2, does (n = 15) were GnRH-stimulated and naturally mated. Samples were retrieved from the internal reproductive tracts (cervix-to-infundibulum) 20 h post-treatment (Experiment 1) or sequentially collected at 10, 24, 36, 68, or 72 h post-mating (Experiment 2, 3 does/period). All samples were processed for gene expression analysis by quantitative PCR. Data showed an upregulation of endometrial CTEN and NGF by NM, but not by SP-AI. The findings suggest that the NGF gene affects the reproductive tract of the doe during ovulation and beyond, influencing the maternal environment during early embryonic development. MDPI 2020-07-07 /pmc/articles/PMC7397043/ /pubmed/32645906 http://dx.doi.org/10.3390/genes11070758 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Gardela, Jaume
Jauregi-Miguel, Amaia
Martinez, Cristina A.
Rodriguez-Martinez, Heriberto
Lopez-Bejar, Manel
Alvarez-Rodriguez, Manuel
Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits
title Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits
title_full Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits
title_fullStr Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits
title_full_unstemmed Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits
title_short Semen Modulates the Expression of NGF, ABHD2, VCAN, and CTEN in the Reproductive Tract of Female Rabbits
title_sort semen modulates the expression of ngf, abhd2, vcan, and cten in the reproductive tract of female rabbits
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397043/
https://www.ncbi.nlm.nih.gov/pubmed/32645906
http://dx.doi.org/10.3390/genes11070758
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