Cargando…

Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton

The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological funct...

Descripción completa

Detalles Bibliográficos
Autores principales: Zhang, Li, Zhang, Yao, Fan, Yijie, Guo, Haixia, Guo, Huihui, Wu, Jianfei, Wang, Hongmei, Zhao, Yunlei, Lian, Xin, Gou, Zhongyuan, Sun, Yuxiao, Zheng, Congcong, Chen, Cuixia, Zeng, Fanchang
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397050/
https://www.ncbi.nlm.nih.gov/pubmed/32659993
http://dx.doi.org/10.3390/genes11070772
_version_ 1783565696819527680
author Zhang, Li
Zhang, Yao
Fan, Yijie
Guo, Haixia
Guo, Huihui
Wu, Jianfei
Wang, Hongmei
Zhao, Yunlei
Lian, Xin
Gou, Zhongyuan
Sun, Yuxiao
Zheng, Congcong
Chen, Cuixia
Zeng, Fanchang
author_facet Zhang, Li
Zhang, Yao
Fan, Yijie
Guo, Haixia
Guo, Huihui
Wu, Jianfei
Wang, Hongmei
Zhao, Yunlei
Lian, Xin
Gou, Zhongyuan
Sun, Yuxiao
Zheng, Congcong
Chen, Cuixia
Zeng, Fanchang
author_sort Zhang, Li
collection PubMed
description The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological function mode in plants. Based on the results of the current investigation, the GhLETM1 gene crucially affects filament elongation and anther dehiscence of the stamen in cotton. Both excessive and lower expression of the GhLETM1 gene lead to defective stamen development, resulting in shortened filaments and indehiscent anthers with pollen abortion. The results also showed that the phenotype of the shortened filaments was negatively correlated with anther defects in the seesaw model under the ectopic expression of GhLETM1. Moreover, our results notably indicated that the gene requires accurate expression and exhibits a sensitive dose effect for its proper function. This report has important fundamental and practical significance in crop science, and has crucial prospects for genetic engineering of new cytoplasmic male sterility lines and breeding of crop hybrid varieties.
format Online
Article
Text
id pubmed-7397050
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-73970502020-08-05 Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton Zhang, Li Zhang, Yao Fan, Yijie Guo, Haixia Guo, Huihui Wu, Jianfei Wang, Hongmei Zhao, Yunlei Lian, Xin Gou, Zhongyuan Sun, Yuxiao Zheng, Congcong Chen, Cuixia Zeng, Fanchang Genes (Basel) Communication The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological function mode in plants. Based on the results of the current investigation, the GhLETM1 gene crucially affects filament elongation and anther dehiscence of the stamen in cotton. Both excessive and lower expression of the GhLETM1 gene lead to defective stamen development, resulting in shortened filaments and indehiscent anthers with pollen abortion. The results also showed that the phenotype of the shortened filaments was negatively correlated with anther defects in the seesaw model under the ectopic expression of GhLETM1. Moreover, our results notably indicated that the gene requires accurate expression and exhibits a sensitive dose effect for its proper function. This report has important fundamental and practical significance in crop science, and has crucial prospects for genetic engineering of new cytoplasmic male sterility lines and breeding of crop hybrid varieties. MDPI 2020-07-09 /pmc/articles/PMC7397050/ /pubmed/32659993 http://dx.doi.org/10.3390/genes11070772 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Communication
Zhang, Li
Zhang, Yao
Fan, Yijie
Guo, Haixia
Guo, Huihui
Wu, Jianfei
Wang, Hongmei
Zhao, Yunlei
Lian, Xin
Gou, Zhongyuan
Sun, Yuxiao
Zheng, Congcong
Chen, Cuixia
Zeng, Fanchang
Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton
title Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton
title_full Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton
title_fullStr Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton
title_full_unstemmed Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton
title_short Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton
title_sort ectopic expressions of the ghletm1 gene reveal sensitive dose effects on precise stamen development and male fertility in cotton
topic Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397050/
https://www.ncbi.nlm.nih.gov/pubmed/32659993
http://dx.doi.org/10.3390/genes11070772
work_keys_str_mv AT zhangli ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT zhangyao ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT fanyijie ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT guohaixia ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT guohuihui ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT wujianfei ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT wanghongmei ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT zhaoyunlei ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT lianxin ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT gouzhongyuan ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT sunyuxiao ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT zhengcongcong ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT chencuixia ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton
AT zengfanchang ectopicexpressionsoftheghletm1generevealsensitivedoseeffectsonprecisestamendevelopmentandmalefertilityincotton