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Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton
The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological funct...
Autores principales: | , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397050/ https://www.ncbi.nlm.nih.gov/pubmed/32659993 http://dx.doi.org/10.3390/genes11070772 |
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author | Zhang, Li Zhang, Yao Fan, Yijie Guo, Haixia Guo, Huihui Wu, Jianfei Wang, Hongmei Zhao, Yunlei Lian, Xin Gou, Zhongyuan Sun, Yuxiao Zheng, Congcong Chen, Cuixia Zeng, Fanchang |
author_facet | Zhang, Li Zhang, Yao Fan, Yijie Guo, Haixia Guo, Huihui Wu, Jianfei Wang, Hongmei Zhao, Yunlei Lian, Xin Gou, Zhongyuan Sun, Yuxiao Zheng, Congcong Chen, Cuixia Zeng, Fanchang |
author_sort | Zhang, Li |
collection | PubMed |
description | The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological function mode in plants. Based on the results of the current investigation, the GhLETM1 gene crucially affects filament elongation and anther dehiscence of the stamen in cotton. Both excessive and lower expression of the GhLETM1 gene lead to defective stamen development, resulting in shortened filaments and indehiscent anthers with pollen abortion. The results also showed that the phenotype of the shortened filaments was negatively correlated with anther defects in the seesaw model under the ectopic expression of GhLETM1. Moreover, our results notably indicated that the gene requires accurate expression and exhibits a sensitive dose effect for its proper function. This report has important fundamental and practical significance in crop science, and has crucial prospects for genetic engineering of new cytoplasmic male sterility lines and breeding of crop hybrid varieties. |
format | Online Article Text |
id | pubmed-7397050 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-73970502020-08-05 Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton Zhang, Li Zhang, Yao Fan, Yijie Guo, Haixia Guo, Huihui Wu, Jianfei Wang, Hongmei Zhao, Yunlei Lian, Xin Gou, Zhongyuan Sun, Yuxiao Zheng, Congcong Chen, Cuixia Zeng, Fanchang Genes (Basel) Communication The homologous leucine zipper/EF-hand-containing transmembranes (LETMs) are highly conserved across a broad range of eukaryotic organisms. The LETM functional characteristics involved in biological process have been identified primarily in animals, but little is known about the LETM biological function mode in plants. Based on the results of the current investigation, the GhLETM1 gene crucially affects filament elongation and anther dehiscence of the stamen in cotton. Both excessive and lower expression of the GhLETM1 gene lead to defective stamen development, resulting in shortened filaments and indehiscent anthers with pollen abortion. The results also showed that the phenotype of the shortened filaments was negatively correlated with anther defects in the seesaw model under the ectopic expression of GhLETM1. Moreover, our results notably indicated that the gene requires accurate expression and exhibits a sensitive dose effect for its proper function. This report has important fundamental and practical significance in crop science, and has crucial prospects for genetic engineering of new cytoplasmic male sterility lines and breeding of crop hybrid varieties. MDPI 2020-07-09 /pmc/articles/PMC7397050/ /pubmed/32659993 http://dx.doi.org/10.3390/genes11070772 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Communication Zhang, Li Zhang, Yao Fan, Yijie Guo, Haixia Guo, Huihui Wu, Jianfei Wang, Hongmei Zhao, Yunlei Lian, Xin Gou, Zhongyuan Sun, Yuxiao Zheng, Congcong Chen, Cuixia Zeng, Fanchang Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton |
title | Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton |
title_full | Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton |
title_fullStr | Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton |
title_full_unstemmed | Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton |
title_short | Ectopic Expressions of the GhLETM1 Gene Reveal Sensitive Dose Effects on Precise Stamen Development and Male Fertility in Cotton |
title_sort | ectopic expressions of the ghletm1 gene reveal sensitive dose effects on precise stamen development and male fertility in cotton |
topic | Communication |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397050/ https://www.ncbi.nlm.nih.gov/pubmed/32659993 http://dx.doi.org/10.3390/genes11070772 |
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