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Versatile Polypeptide-Functionalized Plasmonic Paper as Synergistic Biocompatible and Antimicrobial Nanoplatform

Nowadays, thanks to nanotechnological progress, which itself guides us more and more closely toward not only the efficient design of innovative nanomaterials or nanostructures, but to the improvement of their functionality, we benefit from an important asset in the battle against pathogenic illnesse...

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Autores principales: Tie, Leopold, Răileanu, Mina, Bacalum, Mihaela, Codita, Irina, Negrea, Ștefania Mădălina, Caracoti, Costin Ștefan, Drăgulescu, Elena-Carmina, Campu, Andreea, Astilean, Simion, Focsan, Monica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397136/
https://www.ncbi.nlm.nih.gov/pubmed/32668589
http://dx.doi.org/10.3390/molecules25143182
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author Tie, Leopold
Răileanu, Mina
Bacalum, Mihaela
Codita, Irina
Negrea, Ștefania Mădălina
Caracoti, Costin Ștefan
Drăgulescu, Elena-Carmina
Campu, Andreea
Astilean, Simion
Focsan, Monica
author_facet Tie, Leopold
Răileanu, Mina
Bacalum, Mihaela
Codita, Irina
Negrea, Ștefania Mădălina
Caracoti, Costin Ștefan
Drăgulescu, Elena-Carmina
Campu, Andreea
Astilean, Simion
Focsan, Monica
author_sort Tie, Leopold
collection PubMed
description Nowadays, thanks to nanotechnological progress, which itself guides us more and more closely toward not only the efficient design of innovative nanomaterials or nanostructures, but to the improvement of their functionality, we benefit from an important asset in the battle against pathogenic illnesses. Herein, we report a versatile biocompatible plasmonic nanoplatform based on a Whatman paper incorporating positively-charged gold nanospherical particles via the immersion approach. The morphological characterization of the as-engineered-plasmonic paper was examined by SEM (scanning electron microscopy) and HRTEM (high-resolution transmission electron microscopy) investigations, while its surface chemical modification with a synthetic polypeptide, specifically RRWHRWWRR-NH2 (P2), was proved by monitoring the plasmonic response of loaded gold nanospheres and the emission signal of P2 via fluorescence spectroscopy. The as-functionalized plasmonic paper is non-cytotoxic towards BJ fibroblast human cells at bactericidal concentrations. Finally, the antimicrobial activity of the P2-functionalized plasmonic paper on both planktonic bacteria and biofilms was tested against two reference strains: Gram-positive Bacteria, i.e., Staphylococcus aureus and the Gram-negative Bacteria, i.e., Escherichia coli, determining microbial inhibition of up to 100% for planktonic bacteria. In line with the above presented nanoplatform’s proper design, followed by their functionalization with active antimicrobial peptides, new roads can be open for determining antibiotic-free treatments against different relevant pathogens.
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spelling pubmed-73971362020-08-05 Versatile Polypeptide-Functionalized Plasmonic Paper as Synergistic Biocompatible and Antimicrobial Nanoplatform Tie, Leopold Răileanu, Mina Bacalum, Mihaela Codita, Irina Negrea, Ștefania Mădălina Caracoti, Costin Ștefan Drăgulescu, Elena-Carmina Campu, Andreea Astilean, Simion Focsan, Monica Molecules Article Nowadays, thanks to nanotechnological progress, which itself guides us more and more closely toward not only the efficient design of innovative nanomaterials or nanostructures, but to the improvement of their functionality, we benefit from an important asset in the battle against pathogenic illnesses. Herein, we report a versatile biocompatible plasmonic nanoplatform based on a Whatman paper incorporating positively-charged gold nanospherical particles via the immersion approach. The morphological characterization of the as-engineered-plasmonic paper was examined by SEM (scanning electron microscopy) and HRTEM (high-resolution transmission electron microscopy) investigations, while its surface chemical modification with a synthetic polypeptide, specifically RRWHRWWRR-NH2 (P2), was proved by monitoring the plasmonic response of loaded gold nanospheres and the emission signal of P2 via fluorescence spectroscopy. The as-functionalized plasmonic paper is non-cytotoxic towards BJ fibroblast human cells at bactericidal concentrations. Finally, the antimicrobial activity of the P2-functionalized plasmonic paper on both planktonic bacteria and biofilms was tested against two reference strains: Gram-positive Bacteria, i.e., Staphylococcus aureus and the Gram-negative Bacteria, i.e., Escherichia coli, determining microbial inhibition of up to 100% for planktonic bacteria. In line with the above presented nanoplatform’s proper design, followed by their functionalization with active antimicrobial peptides, new roads can be open for determining antibiotic-free treatments against different relevant pathogens. MDPI 2020-07-13 /pmc/articles/PMC7397136/ /pubmed/32668589 http://dx.doi.org/10.3390/molecules25143182 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tie, Leopold
Răileanu, Mina
Bacalum, Mihaela
Codita, Irina
Negrea, Ștefania Mădălina
Caracoti, Costin Ștefan
Drăgulescu, Elena-Carmina
Campu, Andreea
Astilean, Simion
Focsan, Monica
Versatile Polypeptide-Functionalized Plasmonic Paper as Synergistic Biocompatible and Antimicrobial Nanoplatform
title Versatile Polypeptide-Functionalized Plasmonic Paper as Synergistic Biocompatible and Antimicrobial Nanoplatform
title_full Versatile Polypeptide-Functionalized Plasmonic Paper as Synergistic Biocompatible and Antimicrobial Nanoplatform
title_fullStr Versatile Polypeptide-Functionalized Plasmonic Paper as Synergistic Biocompatible and Antimicrobial Nanoplatform
title_full_unstemmed Versatile Polypeptide-Functionalized Plasmonic Paper as Synergistic Biocompatible and Antimicrobial Nanoplatform
title_short Versatile Polypeptide-Functionalized Plasmonic Paper as Synergistic Biocompatible and Antimicrobial Nanoplatform
title_sort versatile polypeptide-functionalized plasmonic paper as synergistic biocompatible and antimicrobial nanoplatform
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397136/
https://www.ncbi.nlm.nih.gov/pubmed/32668589
http://dx.doi.org/10.3390/molecules25143182
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