Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea)

Dibothriocephalus dendriticus is one of the causative agents of the fish-borne zoonosis diphyllobothriosis. Polymorphic microsatellite markers were originally developed for future genetic studies using microsatellite library screening and next-generation sequencing (NGS). Out of 128 microsatellite c...

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Autores principales: Bazsalovicsová, Eva, Minárik, Gabriel, Šoltys, Katarína, Radačovská, Alžbeta, Kuhn, Jesper A., Karlsbakk, Egil, Skírnisson, Karl, Králová-Hromadová, Ivica
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Technical Note
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397143/
https://www.ncbi.nlm.nih.gov/pubmed/32664678
http://dx.doi.org/10.3390/genes11070782
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author Bazsalovicsová, Eva
Minárik, Gabriel
Šoltys, Katarína
Radačovská, Alžbeta
Kuhn, Jesper A.
Karlsbakk, Egil
Skírnisson, Karl
Králová-Hromadová, Ivica
author_facet Bazsalovicsová, Eva
Minárik, Gabriel
Šoltys, Katarína
Radačovská, Alžbeta
Kuhn, Jesper A.
Karlsbakk, Egil
Skírnisson, Karl
Králová-Hromadová, Ivica
author_sort Bazsalovicsová, Eva
collection PubMed
description Dibothriocephalus dendriticus is one of the causative agents of the fish-borne zoonosis diphyllobothriosis. Polymorphic microsatellite markers were originally developed for future genetic studies using microsatellite library screening and next-generation sequencing (NGS). Out of 128 microsatellite candidates selected after NGS analysis, 126 yielded PCR products of the expected size. A declared repetitive motif was confirmed in 92 loci by Sanger sequencing. The level of polymorphism was tested by fragment analysis. Statistical tests for observed and expected heterozygosities and deviations from Hardy–Weinberg equilibrium revealed 14 polymorphic microsatellite loci suitable for studies on the finer genetic structure of global populations of D. dendriticus.
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spelling pubmed-73971432020-08-05 Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea) Bazsalovicsová, Eva Minárik, Gabriel Šoltys, Katarína Radačovská, Alžbeta Kuhn, Jesper A. Karlsbakk, Egil Skírnisson, Karl Králová-Hromadová, Ivica Genes (Basel) Technical Note Dibothriocephalus dendriticus is one of the causative agents of the fish-borne zoonosis diphyllobothriosis. Polymorphic microsatellite markers were originally developed for future genetic studies using microsatellite library screening and next-generation sequencing (NGS). Out of 128 microsatellite candidates selected after NGS analysis, 126 yielded PCR products of the expected size. A declared repetitive motif was confirmed in 92 loci by Sanger sequencing. The level of polymorphism was tested by fragment analysis. Statistical tests for observed and expected heterozygosities and deviations from Hardy–Weinberg equilibrium revealed 14 polymorphic microsatellite loci suitable for studies on the finer genetic structure of global populations of D. dendriticus. MDPI 2020-07-12 /pmc/articles/PMC7397143/ /pubmed/32664678 http://dx.doi.org/10.3390/genes11070782 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Technical Note
Bazsalovicsová, Eva
Minárik, Gabriel
Šoltys, Katarína
Radačovská, Alžbeta
Kuhn, Jesper A.
Karlsbakk, Egil
Skírnisson, Karl
Králová-Hromadová, Ivica
Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea)
title Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea)
title_full Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea)
title_fullStr Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea)
title_full_unstemmed Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea)
title_short Development of 14 Microsatellite Markers for Zoonotic Tapeworm Dibothriocephalus dendriticus (Cestoda: Diphyllobothriidea)
title_sort development of 14 microsatellite markers for zoonotic tapeworm dibothriocephalus dendriticus (cestoda: diphyllobothriidea)
topic Technical Note
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397143/
https://www.ncbi.nlm.nih.gov/pubmed/32664678
http://dx.doi.org/10.3390/genes11070782
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