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Identification of Loci and Pathways Associated with Heifer Conception Rate in U.S. Holsteins

Heifer conception rate (HCR) is defined as the percentage of inseminated heifers that become pregnant at each service. The genome-wide association analyses in this study focused on identifying the loci associated with Holstein heifer (n = 2013) conception rate at first service (HCR1) and the number...

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Autores principales: Galliou, Justine M., Kiser, Jennifer N., Oliver, Kayleen F., Seabury, Christopher M., Moraes, Joao G. N., Burns, Gregory W., Spencer, Thomas E., Dalton, Joseph, Neibergs, Holly L.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397161/
https://www.ncbi.nlm.nih.gov/pubmed/32650431
http://dx.doi.org/10.3390/genes11070767
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author Galliou, Justine M.
Kiser, Jennifer N.
Oliver, Kayleen F.
Seabury, Christopher M.
Moraes, Joao G. N.
Burns, Gregory W.
Spencer, Thomas E.
Dalton, Joseph
Neibergs, Holly L.
author_facet Galliou, Justine M.
Kiser, Jennifer N.
Oliver, Kayleen F.
Seabury, Christopher M.
Moraes, Joao G. N.
Burns, Gregory W.
Spencer, Thomas E.
Dalton, Joseph
Neibergs, Holly L.
author_sort Galliou, Justine M.
collection PubMed
description Heifer conception rate (HCR) is defined as the percentage of inseminated heifers that become pregnant at each service. The genome-wide association analyses in this study focused on identifying the loci associated with Holstein heifer (n = 2013) conception rate at first service (HCR1) and the number of times bred (TBRD) to achieve a pregnancy. There were 348 unique loci associated (p < 5 × 10(−8)) with HCR1 and 615 unique loci associated (p < 5 × 10(−8)) with TBRD. The two phenotypes shared 302 loci, and 56 loci were validated in independent cattle populations. There were 52 transcription factor binding sites (TFBS) and 552 positional candidate genes identified in the HCR1- and TBRD-associated loci. The positional candidate genes and the TFBS associated with HCR1 and TBRD were used in the ingenuity pathway analysis (IPA). In the IPA, 11 pathways, 207 master regulators and 11 upstream regulators were associated (p < 1.23 × 10(−5)) with HCR1 and TBRD. The validated loci associated with both HCR1 and TBRD make good candidates for genomic selection and further investigations to elucidate the mechanisms associated with subfertility and infertility.
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spelling pubmed-73971612020-08-16 Identification of Loci and Pathways Associated with Heifer Conception Rate in U.S. Holsteins Galliou, Justine M. Kiser, Jennifer N. Oliver, Kayleen F. Seabury, Christopher M. Moraes, Joao G. N. Burns, Gregory W. Spencer, Thomas E. Dalton, Joseph Neibergs, Holly L. Genes (Basel) Article Heifer conception rate (HCR) is defined as the percentage of inseminated heifers that become pregnant at each service. The genome-wide association analyses in this study focused on identifying the loci associated with Holstein heifer (n = 2013) conception rate at first service (HCR1) and the number of times bred (TBRD) to achieve a pregnancy. There were 348 unique loci associated (p < 5 × 10(−8)) with HCR1 and 615 unique loci associated (p < 5 × 10(−8)) with TBRD. The two phenotypes shared 302 loci, and 56 loci were validated in independent cattle populations. There were 52 transcription factor binding sites (TFBS) and 552 positional candidate genes identified in the HCR1- and TBRD-associated loci. The positional candidate genes and the TFBS associated with HCR1 and TBRD were used in the ingenuity pathway analysis (IPA). In the IPA, 11 pathways, 207 master regulators and 11 upstream regulators were associated (p < 1.23 × 10(−5)) with HCR1 and TBRD. The validated loci associated with both HCR1 and TBRD make good candidates for genomic selection and further investigations to elucidate the mechanisms associated with subfertility and infertility. MDPI 2020-07-08 /pmc/articles/PMC7397161/ /pubmed/32650431 http://dx.doi.org/10.3390/genes11070767 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Galliou, Justine M.
Kiser, Jennifer N.
Oliver, Kayleen F.
Seabury, Christopher M.
Moraes, Joao G. N.
Burns, Gregory W.
Spencer, Thomas E.
Dalton, Joseph
Neibergs, Holly L.
Identification of Loci and Pathways Associated with Heifer Conception Rate in U.S. Holsteins
title Identification of Loci and Pathways Associated with Heifer Conception Rate in U.S. Holsteins
title_full Identification of Loci and Pathways Associated with Heifer Conception Rate in U.S. Holsteins
title_fullStr Identification of Loci and Pathways Associated with Heifer Conception Rate in U.S. Holsteins
title_full_unstemmed Identification of Loci and Pathways Associated with Heifer Conception Rate in U.S. Holsteins
title_short Identification of Loci and Pathways Associated with Heifer Conception Rate in U.S. Holsteins
title_sort identification of loci and pathways associated with heifer conception rate in u.s. holsteins
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397161/
https://www.ncbi.nlm.nih.gov/pubmed/32650431
http://dx.doi.org/10.3390/genes11070767
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