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Profile of the Nicotinic Cholinergic Receptor Alpha 7 Subunit Gene Expression is Associated with Response to Varenicline Treatment

Introduction: Smoking is considered the leading cause of preventable morbidity and mortality worldwide. Studies have sought to identify predictors of response to smoking cessation treatments. The aim of this study was to analyze a possible association of target gene expression for smoking cessation...

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Autores principales: Santos, Juliana Rocha, Tomaz, Paulo Roberto Xavier, Scholz, Jaqueline Ribeiro, Gaya, Patrícia Viviane, Abe, Tânia Ogawa, Krieger, José Eduardo, Pereira, Alexandre Costa, Santos, Paulo Caleb Júnior de Lima
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
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Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397196/
https://www.ncbi.nlm.nih.gov/pubmed/32640505
http://dx.doi.org/10.3390/genes11070746
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author Santos, Juliana Rocha
Tomaz, Paulo Roberto Xavier
Scholz, Jaqueline Ribeiro
Gaya, Patrícia Viviane
Abe, Tânia Ogawa
Krieger, José Eduardo
Pereira, Alexandre Costa
Santos, Paulo Caleb Júnior de Lima
author_facet Santos, Juliana Rocha
Tomaz, Paulo Roberto Xavier
Scholz, Jaqueline Ribeiro
Gaya, Patrícia Viviane
Abe, Tânia Ogawa
Krieger, José Eduardo
Pereira, Alexandre Costa
Santos, Paulo Caleb Júnior de Lima
author_sort Santos, Juliana Rocha
collection PubMed
description Introduction: Smoking is considered the leading cause of preventable morbidity and mortality worldwide. Studies have sought to identify predictors of response to smoking cessation treatments. The aim of this study was to analyze a possible association of target gene expression for smoking cessation with varenicline. Methods: We included 74 smokers starting treatment with varenicline. Gene expression analysis was performed through the custom RT² Profiler qPCR array assay, including 17 genes. Times for sample collection were before the start of therapy (T0) and two weeks (T2) and four weeks (T4) after the start of treatment. Results: For gene expression analysis, we selected 14 patients who had success and 13 patients resistant to varenicline treatment. Success was considered to be when a patient achieved tobacco abstinence until the fourth week of treatment and resistant was when a patient had not stopped smoking as of the fourth week of treatment. We observed a significant difference for CHRNA7 gene expression: in the resistant group, samples from T2 and T4 had lower expression compared with T0 (fold change: 0.38, P = 0.007; fold change: 0.67, P = 0.004; respectively). Conclusion: This exploratory clinical study, searching for a possible predictor of effectiveness for varenicline, reaffirmed the association of the α7 nAChR subunit for nicotine dependence and smoking therapy effectiveness with varenicline.
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spelling pubmed-73971962020-08-16 Profile of the Nicotinic Cholinergic Receptor Alpha 7 Subunit Gene Expression is Associated with Response to Varenicline Treatment Santos, Juliana Rocha Tomaz, Paulo Roberto Xavier Scholz, Jaqueline Ribeiro Gaya, Patrícia Viviane Abe, Tânia Ogawa Krieger, José Eduardo Pereira, Alexandre Costa Santos, Paulo Caleb Júnior de Lima Genes (Basel) Article Introduction: Smoking is considered the leading cause of preventable morbidity and mortality worldwide. Studies have sought to identify predictors of response to smoking cessation treatments. The aim of this study was to analyze a possible association of target gene expression for smoking cessation with varenicline. Methods: We included 74 smokers starting treatment with varenicline. Gene expression analysis was performed through the custom RT² Profiler qPCR array assay, including 17 genes. Times for sample collection were before the start of therapy (T0) and two weeks (T2) and four weeks (T4) after the start of treatment. Results: For gene expression analysis, we selected 14 patients who had success and 13 patients resistant to varenicline treatment. Success was considered to be when a patient achieved tobacco abstinence until the fourth week of treatment and resistant was when a patient had not stopped smoking as of the fourth week of treatment. We observed a significant difference for CHRNA7 gene expression: in the resistant group, samples from T2 and T4 had lower expression compared with T0 (fold change: 0.38, P = 0.007; fold change: 0.67, P = 0.004; respectively). Conclusion: This exploratory clinical study, searching for a possible predictor of effectiveness for varenicline, reaffirmed the association of the α7 nAChR subunit for nicotine dependence and smoking therapy effectiveness with varenicline. MDPI 2020-07-06 /pmc/articles/PMC7397196/ /pubmed/32640505 http://dx.doi.org/10.3390/genes11070746 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Santos, Juliana Rocha
Tomaz, Paulo Roberto Xavier
Scholz, Jaqueline Ribeiro
Gaya, Patrícia Viviane
Abe, Tânia Ogawa
Krieger, José Eduardo
Pereira, Alexandre Costa
Santos, Paulo Caleb Júnior de Lima
Profile of the Nicotinic Cholinergic Receptor Alpha 7 Subunit Gene Expression is Associated with Response to Varenicline Treatment
title Profile of the Nicotinic Cholinergic Receptor Alpha 7 Subunit Gene Expression is Associated with Response to Varenicline Treatment
title_full Profile of the Nicotinic Cholinergic Receptor Alpha 7 Subunit Gene Expression is Associated with Response to Varenicline Treatment
title_fullStr Profile of the Nicotinic Cholinergic Receptor Alpha 7 Subunit Gene Expression is Associated with Response to Varenicline Treatment
title_full_unstemmed Profile of the Nicotinic Cholinergic Receptor Alpha 7 Subunit Gene Expression is Associated with Response to Varenicline Treatment
title_short Profile of the Nicotinic Cholinergic Receptor Alpha 7 Subunit Gene Expression is Associated with Response to Varenicline Treatment
title_sort profile of the nicotinic cholinergic receptor alpha 7 subunit gene expression is associated with response to varenicline treatment
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397196/
https://www.ncbi.nlm.nih.gov/pubmed/32640505
http://dx.doi.org/10.3390/genes11070746
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