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EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet

Dietary intake of eicosapentaenoic/docosahexaenoic acid (EPA/DHA) reduces insulin resistance and hepatic manifestations through the regulation of metabolism in the liver. Obese mice present insulin resistance and lipid accumulation in intracellular lipid droplets (LDs). LD-associated proteins perili...

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Autores principales: Espinosa, Alejandra, Ross, Andrés, Dovale-Rosabal, Gretel, Pino-de la Fuente, Francisco, Uribe-Oporto, Ernesto, Sacristán, Camila, Ruiz, Paulina, Valenzuela, Rodrigo, Romero, Nalda, Aubourg, Santiago P., Rodríguez, Alicia
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397222/
https://www.ncbi.nlm.nih.gov/pubmed/32698439
http://dx.doi.org/10.3390/molecules25143289
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author Espinosa, Alejandra
Ross, Andrés
Dovale-Rosabal, Gretel
Pino-de la Fuente, Francisco
Uribe-Oporto, Ernesto
Sacristán, Camila
Ruiz, Paulina
Valenzuela, Rodrigo
Romero, Nalda
Aubourg, Santiago P.
Rodríguez, Alicia
author_facet Espinosa, Alejandra
Ross, Andrés
Dovale-Rosabal, Gretel
Pino-de la Fuente, Francisco
Uribe-Oporto, Ernesto
Sacristán, Camila
Ruiz, Paulina
Valenzuela, Rodrigo
Romero, Nalda
Aubourg, Santiago P.
Rodríguez, Alicia
author_sort Espinosa, Alejandra
collection PubMed
description Dietary intake of eicosapentaenoic/docosahexaenoic acid (EPA/DHA) reduces insulin resistance and hepatic manifestations through the regulation of metabolism in the liver. Obese mice present insulin resistance and lipid accumulation in intracellular lipid droplets (LDs). LD-associated proteins perilipin (Plin) have an essential role in both adipogenesis and lipolysis; Plin5 regulates lipolysis and thus contributes to fat oxidation. The purpose of this study was to compare the effects of deodorized refined salmon oil (DSO) and its polyunsaturated fatty acids concentrate (CPUFA) containing EPA and DHA, obtained by complexing with urea, on obesity-induced metabolic alteration. CPUFA maximum content was determined using the Box–Behnken experimental design based on Surface Response Methodology. The optimized CPUFA was administered to high-fat diet (HFD)-fed mice (200 mg/kg/day of EPA + DHA) for 8 weeks. No significant differences (p > 0.05) in cholesterol, glycemia, LDs or transaminase content were found. Fasting insulin and hepatic Plin5 protein level increased in the group supplemented with the EPA + DHA optimized product (38.35 g/100 g total fatty acids) compared to obese mice without fish oil supplementation. The results suggest that processing salmon oil by urea concentration can generate an EPA+DHA dose useful to prevent the increase of fasting insulin and the decrease of Plin5 in the liver of insulin-resistant mice.
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spelling pubmed-73972222020-08-16 EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet Espinosa, Alejandra Ross, Andrés Dovale-Rosabal, Gretel Pino-de la Fuente, Francisco Uribe-Oporto, Ernesto Sacristán, Camila Ruiz, Paulina Valenzuela, Rodrigo Romero, Nalda Aubourg, Santiago P. Rodríguez, Alicia Molecules Article Dietary intake of eicosapentaenoic/docosahexaenoic acid (EPA/DHA) reduces insulin resistance and hepatic manifestations through the regulation of metabolism in the liver. Obese mice present insulin resistance and lipid accumulation in intracellular lipid droplets (LDs). LD-associated proteins perilipin (Plin) have an essential role in both adipogenesis and lipolysis; Plin5 regulates lipolysis and thus contributes to fat oxidation. The purpose of this study was to compare the effects of deodorized refined salmon oil (DSO) and its polyunsaturated fatty acids concentrate (CPUFA) containing EPA and DHA, obtained by complexing with urea, on obesity-induced metabolic alteration. CPUFA maximum content was determined using the Box–Behnken experimental design based on Surface Response Methodology. The optimized CPUFA was administered to high-fat diet (HFD)-fed mice (200 mg/kg/day of EPA + DHA) for 8 weeks. No significant differences (p > 0.05) in cholesterol, glycemia, LDs or transaminase content were found. Fasting insulin and hepatic Plin5 protein level increased in the group supplemented with the EPA + DHA optimized product (38.35 g/100 g total fatty acids) compared to obese mice without fish oil supplementation. The results suggest that processing salmon oil by urea concentration can generate an EPA+DHA dose useful to prevent the increase of fasting insulin and the decrease of Plin5 in the liver of insulin-resistant mice. MDPI 2020-07-20 /pmc/articles/PMC7397222/ /pubmed/32698439 http://dx.doi.org/10.3390/molecules25143289 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Espinosa, Alejandra
Ross, Andrés
Dovale-Rosabal, Gretel
Pino-de la Fuente, Francisco
Uribe-Oporto, Ernesto
Sacristán, Camila
Ruiz, Paulina
Valenzuela, Rodrigo
Romero, Nalda
Aubourg, Santiago P.
Rodríguez, Alicia
EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet
title EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet
title_full EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet
title_fullStr EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet
title_full_unstemmed EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet
title_short EPA/DHA Concentrate by Urea Complexation Decreases Hyperinsulinemia and Increases Plin5 in the Liver of Mice Fed a High-Fat Diet
title_sort epa/dha concentrate by urea complexation decreases hyperinsulinemia and increases plin5 in the liver of mice fed a high-fat diet
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397222/
https://www.ncbi.nlm.nih.gov/pubmed/32698439
http://dx.doi.org/10.3390/molecules25143289
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