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Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny

Two species from the families Acipenseridae and Polyodontidae, Russian sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833; functional tetraploid) and American paddlefish (Polyodon spathula, Walbaum 1792, functional diploid) were hybridized. The hybridization was repeated using eggs from...

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Autores principales: Káldy, Jenő, Mozsár, Attila, Fazekas, Gyöngyvér, Farkas, Móni, Fazekas, Dorottya Lilla, Fazekas, Georgina Lea, Goda, Katalin, Gyöngy, Zsuzsanna, Kovács, Balázs, Semmens, Kenneth, Bercsényi, Miklós, Molnár, Mariann, Patakiné Várkonyi, Eszter
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397225/
https://www.ncbi.nlm.nih.gov/pubmed/32640744
http://dx.doi.org/10.3390/genes11070753
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author Káldy, Jenő
Mozsár, Attila
Fazekas, Gyöngyvér
Farkas, Móni
Fazekas, Dorottya Lilla
Fazekas, Georgina Lea
Goda, Katalin
Gyöngy, Zsuzsanna
Kovács, Balázs
Semmens, Kenneth
Bercsényi, Miklós
Molnár, Mariann
Patakiné Várkonyi, Eszter
author_facet Káldy, Jenő
Mozsár, Attila
Fazekas, Gyöngyvér
Farkas, Móni
Fazekas, Dorottya Lilla
Fazekas, Georgina Lea
Goda, Katalin
Gyöngy, Zsuzsanna
Kovács, Balázs
Semmens, Kenneth
Bercsényi, Miklós
Molnár, Mariann
Patakiné Várkonyi, Eszter
author_sort Káldy, Jenő
collection PubMed
description Two species from the families Acipenseridae and Polyodontidae, Russian sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833; functional tetraploid) and American paddlefish (Polyodon spathula, Walbaum 1792, functional diploid) were hybridized. The hybridization was repeated using eggs from three sturgeon and sperm from four paddlefish individuals. Survival in all hybrid family groups ranged from 62% to 74% 30 days after hatching. This was the first successful hybridization between these two species and between members of the family Acipenseridae and Polyodontidae. Flow cytometry and chromosome analysis revealed two ploidy levels in hybrids. The chromosome numbers of the hybrids ranged between 156–184 and 300–310, in “functional” triploids and “functional” pentaploids, respectively. The hybrid origin and the ploidy levels were also confirmed by microsatellite analyses. In hybrids, the size and the number of dorsal and ventral scutes correlated with the ploidy levels as well as with the calculated ratio of the maternal and paternal chromosome sets. An extra haploid cell lineage was found in three hybrid individuals irrespective of the ploidy level, suggesting polyspermy. Although the growth performance showed high variance in hybrids (mean: 1.2 kg, SD: 0.55), many individuals reached a size of approximately 1 kg by the age of one year under intensive rearing conditions.
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spelling pubmed-73972252020-08-16 Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny Káldy, Jenő Mozsár, Attila Fazekas, Gyöngyvér Farkas, Móni Fazekas, Dorottya Lilla Fazekas, Georgina Lea Goda, Katalin Gyöngy, Zsuzsanna Kovács, Balázs Semmens, Kenneth Bercsényi, Miklós Molnár, Mariann Patakiné Várkonyi, Eszter Genes (Basel) Article Two species from the families Acipenseridae and Polyodontidae, Russian sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833; functional tetraploid) and American paddlefish (Polyodon spathula, Walbaum 1792, functional diploid) were hybridized. The hybridization was repeated using eggs from three sturgeon and sperm from four paddlefish individuals. Survival in all hybrid family groups ranged from 62% to 74% 30 days after hatching. This was the first successful hybridization between these two species and between members of the family Acipenseridae and Polyodontidae. Flow cytometry and chromosome analysis revealed two ploidy levels in hybrids. The chromosome numbers of the hybrids ranged between 156–184 and 300–310, in “functional” triploids and “functional” pentaploids, respectively. The hybrid origin and the ploidy levels were also confirmed by microsatellite analyses. In hybrids, the size and the number of dorsal and ventral scutes correlated with the ploidy levels as well as with the calculated ratio of the maternal and paternal chromosome sets. An extra haploid cell lineage was found in three hybrid individuals irrespective of the ploidy level, suggesting polyspermy. Although the growth performance showed high variance in hybrids (mean: 1.2 kg, SD: 0.55), many individuals reached a size of approximately 1 kg by the age of one year under intensive rearing conditions. MDPI 2020-07-06 /pmc/articles/PMC7397225/ /pubmed/32640744 http://dx.doi.org/10.3390/genes11070753 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Káldy, Jenő
Mozsár, Attila
Fazekas, Gyöngyvér
Farkas, Móni
Fazekas, Dorottya Lilla
Fazekas, Georgina Lea
Goda, Katalin
Gyöngy, Zsuzsanna
Kovács, Balázs
Semmens, Kenneth
Bercsényi, Miklós
Molnár, Mariann
Patakiné Várkonyi, Eszter
Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny
title Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny
title_full Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny
title_fullStr Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny
title_full_unstemmed Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny
title_short Hybridization of Russian Sturgeon (Acipenser gueldenstaedtii, Brandt and Ratzeberg, 1833) and American Paddlefish (Polyodon spathula, Walbaum 1792) and Evaluation of Their Progeny
title_sort hybridization of russian sturgeon (acipenser gueldenstaedtii, brandt and ratzeberg, 1833) and american paddlefish (polyodon spathula, walbaum 1792) and evaluation of their progeny
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397225/
https://www.ncbi.nlm.nih.gov/pubmed/32640744
http://dx.doi.org/10.3390/genes11070753
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