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Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats

BACKGROUND: We previously reported that the granulocyte colony stimulating factor (G-CSF) ameliorated hepatic steatosis with the enhancement of β-oxidation-related gene expression. However, the mechanisms underlying this process remain unclear. This study aimed to determine whether the improvement o...

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Autores principales: Joo, Hyun-Woo, Song, Yi-Sun, Park, In-Hwa, Shen, Guang-Yin, Seong, Jin-Hee, Shin, Na-Kyoung, Lee, A-Hyeon, Kim, Hyuck, Kim, Kyung-Soo
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Hindawi 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397386/
https://www.ncbi.nlm.nih.gov/pubmed/32775312
http://dx.doi.org/10.1155/2020/2156829
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author Joo, Hyun-Woo
Song, Yi-Sun
Park, In-Hwa
Shen, Guang-Yin
Seong, Jin-Hee
Shin, Na-Kyoung
Lee, A-Hyeon
Kim, Hyuck
Kim, Kyung-Soo
author_facet Joo, Hyun-Woo
Song, Yi-Sun
Park, In-Hwa
Shen, Guang-Yin
Seong, Jin-Hee
Shin, Na-Kyoung
Lee, A-Hyeon
Kim, Hyuck
Kim, Kyung-Soo
author_sort Joo, Hyun-Woo
collection PubMed
description BACKGROUND: We previously reported that the granulocyte colony stimulating factor (G-CSF) ameliorated hepatic steatosis with the enhancement of β-oxidation-related gene expression. However, the mechanisms underlying this process remain unclear. This study aimed to determine whether the improvement of hepatic steatosis by G-CSF was associated with autophagy in a rat model of diabetes. METHODS: Eight rats were fed a standard diet, and 16 rats were fed high-fat diet (HFD) for 5 weeks. All HFD-fed rats were then injected with streptozotocin (STZ). One week later, HFD rats injected with STZ were randomly treated with either G-CSF (200 μg/kg/day; diabetes mellitus (DM)/G-CSF) or saline (DM/saline) for 5 consecutive days. Four weeks later, serum biochemical and histology analyses were conducted. The expression of autophagy-associated proteins was determined by Western blotting. The mRNA expression of β-oxidation-related genes was determined by quantitative real-time polymerase chain reaction. HepG2 cells were cultured under high glucose (HG) conditions with G-CSF treatment, followed by Oil Red O staining for quantification of lipids. RESULTS: Histological analysis showed lower lipid accumulation in the DM/G-CSF group than in the DM/saline-treated rats. Protein levels of LC3 and beclin-1 were higher, and those of p62 were lower in the DM/G-CSF rats than in the DM/saline-treated rats. The mRNA expression of β-oxidation-related genes was higher in DM/G-CSF rats than in the DM/saline-treated rats. Quantification of lipid levels in HepG2 cells cultured with HG and G-CSF treatment revealed no significant differences. CONCLUSIONS: Our data suggested that G-CSF potentially improves hepatic steatosis and autophagy in the liver of diabetic rats.
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spelling pubmed-73973862020-08-07 Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats Joo, Hyun-Woo Song, Yi-Sun Park, In-Hwa Shen, Guang-Yin Seong, Jin-Hee Shin, Na-Kyoung Lee, A-Hyeon Kim, Hyuck Kim, Kyung-Soo Can J Gastroenterol Hepatol Research Article BACKGROUND: We previously reported that the granulocyte colony stimulating factor (G-CSF) ameliorated hepatic steatosis with the enhancement of β-oxidation-related gene expression. However, the mechanisms underlying this process remain unclear. This study aimed to determine whether the improvement of hepatic steatosis by G-CSF was associated with autophagy in a rat model of diabetes. METHODS: Eight rats were fed a standard diet, and 16 rats were fed high-fat diet (HFD) for 5 weeks. All HFD-fed rats were then injected with streptozotocin (STZ). One week later, HFD rats injected with STZ were randomly treated with either G-CSF (200 μg/kg/day; diabetes mellitus (DM)/G-CSF) or saline (DM/saline) for 5 consecutive days. Four weeks later, serum biochemical and histology analyses were conducted. The expression of autophagy-associated proteins was determined by Western blotting. The mRNA expression of β-oxidation-related genes was determined by quantitative real-time polymerase chain reaction. HepG2 cells were cultured under high glucose (HG) conditions with G-CSF treatment, followed by Oil Red O staining for quantification of lipids. RESULTS: Histological analysis showed lower lipid accumulation in the DM/G-CSF group than in the DM/saline-treated rats. Protein levels of LC3 and beclin-1 were higher, and those of p62 were lower in the DM/G-CSF rats than in the DM/saline-treated rats. The mRNA expression of β-oxidation-related genes was higher in DM/G-CSF rats than in the DM/saline-treated rats. Quantification of lipid levels in HepG2 cells cultured with HG and G-CSF treatment revealed no significant differences. CONCLUSIONS: Our data suggested that G-CSF potentially improves hepatic steatosis and autophagy in the liver of diabetic rats. Hindawi 2020-07-24 /pmc/articles/PMC7397386/ /pubmed/32775312 http://dx.doi.org/10.1155/2020/2156829 Text en Copyright © 2020 Hyun-Woo Joo et al. http://creativecommons.org/licenses/by/4.0/ This is an open access article distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Research Article
Joo, Hyun-Woo
Song, Yi-Sun
Park, In-Hwa
Shen, Guang-Yin
Seong, Jin-Hee
Shin, Na-Kyoung
Lee, A-Hyeon
Kim, Hyuck
Kim, Kyung-Soo
Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats
title Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats
title_full Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats
title_fullStr Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats
title_full_unstemmed Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats
title_short Granulocyte Colony Stimulating Factor Ameliorates Hepatic Steatosis Associated with Improvement of Autophagy in Diabetic Rats
title_sort granulocyte colony stimulating factor ameliorates hepatic steatosis associated with improvement of autophagy in diabetic rats
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397386/
https://www.ncbi.nlm.nih.gov/pubmed/32775312
http://dx.doi.org/10.1155/2020/2156829
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