Design and Testing of Vector-Producing HEK293T Cells Bearing a Genomic Deletion of the SV40 T Antigen Coding Region

The use of the human embryonic kidney (HEK) 293T cell line to manufacture vectors for in vivo applications raises safety concerns due to the presence of SV40 T antigen-encoding sequences. We used CRISPR-Cas9 genome editing to remove the SV40 T antigen-encoding sequences from HEK293T cells by transfe...

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Detalles Bibliográficos
Autores principales: Bae, Dahae Hailey, Marino, Michael, Iaffaldano, Brian, Fenstermaker, Sydney, Afione, Sandra, Argaw, Takele, McCright, Jacob, Kwilas, Anna, Chiorini, John A., Timmons, Andrew E., Reiser, Jakob
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Society of Gene & Cell Therapy 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397404/
https://www.ncbi.nlm.nih.gov/pubmed/32775497
http://dx.doi.org/10.1016/j.omtm.2020.07.006

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