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Crystal structure of the Rab-binding domain of Rab11 family-interacting protein 2

The small GTPases Rab11, Rab14 and Rab25 regulate membrane trafficking through the recruitment of Rab11 family-interacting proteins (FIPs) to endocytic compartments. FIPs are multi-domain effector proteins that have a highly conserved Rab-binding domain (RBD) at their C-termini. Several structures o...

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Detalles Bibliográficos
Autores principales: Kearney, Aoife Mairead, Khan, Amir Rafiq
Formato: Online Artículo Texto
Lenguaje:English
Publicado: International Union of Crystallography 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397465/
https://www.ncbi.nlm.nih.gov/pubmed/32744247
http://dx.doi.org/10.1107/S2053230X20009164
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author Kearney, Aoife Mairead
Khan, Amir Rafiq
author_facet Kearney, Aoife Mairead
Khan, Amir Rafiq
author_sort Kearney, Aoife Mairead
collection PubMed
description The small GTPases Rab11, Rab14 and Rab25 regulate membrane trafficking through the recruitment of Rab11 family-interacting proteins (FIPs) to endocytic compartments. FIPs are multi-domain effector proteins that have a highly conserved Rab-binding domain (RBD) at their C-termini. Several structures of complexes of Rab11 with RBDs have previously been determined, including those of Rab11–FIP2 and Rab11–FIP3. In addition, the structures of the Rab14–FIP1 and Rab25–FIP2 complexes have been determined. All of the RBD structures contain a central parallel coiled coil in the RBD that binds to the switch 1 and switch 2 regions of the Rab. Here, the crystal structure of the uncomplexed RBD of FIP2 is presented at 2.3 Å resolution. The structure reveals antiparallel α-helices that associate through polar interactions. These include a remarkable stack of arginine residues within a four-helix bundle in the crystal lattice.
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spelling pubmed-73974652020-08-11 Crystal structure of the Rab-binding domain of Rab11 family-interacting protein 2 Kearney, Aoife Mairead Khan, Amir Rafiq Acta Crystallogr F Struct Biol Commun Research Communications The small GTPases Rab11, Rab14 and Rab25 regulate membrane trafficking through the recruitment of Rab11 family-interacting proteins (FIPs) to endocytic compartments. FIPs are multi-domain effector proteins that have a highly conserved Rab-binding domain (RBD) at their C-termini. Several structures of complexes of Rab11 with RBDs have previously been determined, including those of Rab11–FIP2 and Rab11–FIP3. In addition, the structures of the Rab14–FIP1 and Rab25–FIP2 complexes have been determined. All of the RBD structures contain a central parallel coiled coil in the RBD that binds to the switch 1 and switch 2 regions of the Rab. Here, the crystal structure of the uncomplexed RBD of FIP2 is presented at 2.3 Å resolution. The structure reveals antiparallel α-helices that associate through polar interactions. These include a remarkable stack of arginine residues within a four-helix bundle in the crystal lattice. International Union of Crystallography 2020-07-28 /pmc/articles/PMC7397465/ /pubmed/32744247 http://dx.doi.org/10.1107/S2053230X20009164 Text en © Kearney & Khan 2020 http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution (CC-BY) Licence, which permits unrestricted use, distribution, and reproduction in any medium, provided the original authors and source are cited.http://creativecommons.org/licenses/by/4.0/
spellingShingle Research Communications
Kearney, Aoife Mairead
Khan, Amir Rafiq
Crystal structure of the Rab-binding domain of Rab11 family-interacting protein 2
title Crystal structure of the Rab-binding domain of Rab11 family-interacting protein 2
title_full Crystal structure of the Rab-binding domain of Rab11 family-interacting protein 2
title_fullStr Crystal structure of the Rab-binding domain of Rab11 family-interacting protein 2
title_full_unstemmed Crystal structure of the Rab-binding domain of Rab11 family-interacting protein 2
title_short Crystal structure of the Rab-binding domain of Rab11 family-interacting protein 2
title_sort crystal structure of the rab-binding domain of rab11 family-interacting protein 2
topic Research Communications
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397465/
https://www.ncbi.nlm.nih.gov/pubmed/32744247
http://dx.doi.org/10.1107/S2053230X20009164
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