Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus

Trypanosomes have long been recognised as being amongst the most important protozoan parasites of vertebrates, from both medical and veterinary perspectives. Whilst numerous insect species have been identified as vectors, the role of ticks is less well understood. Here we report the isolation and pa...

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Autores principales: Luu, Lisa, Bown, Kevin J., Palomar, Ana M., Kazimírová, Mária, Bell-Sakyi, Lesley
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Elsevier 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397511/
https://www.ncbi.nlm.nih.gov/pubmed/32723658
http://dx.doi.org/10.1016/j.ttbdis.2020.101501
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author Luu, Lisa
Bown, Kevin J.
Palomar, Ana M.
Kazimírová, Mária
Bell-Sakyi, Lesley
author_facet Luu, Lisa
Bown, Kevin J.
Palomar, Ana M.
Kazimírová, Mária
Bell-Sakyi, Lesley
author_sort Luu, Lisa
collection PubMed
description Trypanosomes have long been recognised as being amongst the most important protozoan parasites of vertebrates, from both medical and veterinary perspectives. Whilst numerous insect species have been identified as vectors, the role of ticks is less well understood. Here we report the isolation and partial molecular characterisation of a novel trypanosome from questing Ixodes ricinus ticks collected in Slovakia. The trypanosome was isolated in tick cell culture and then partially characterised by microscopy and amplification of fragments of the 18S rRNA and 24Sα rDNA genes. Analysis of the resultant sequences suggests that the trypanosome designated as Trypanosoma sp. Bratislava1 may be a new species closely related to several species or strains of trypanosomes isolated from, or detected in, ticks in South America and Asia, and to Trypanosoma caninum isolated from dogs in Brazil. This study highlights the potential involvement of ixodid ticks in the epidemiology of trypanosomes, as well as the use of tick cell lines for isolation of such tick-borne protozoa. Further studies are required to investigate the epidemiology, transmission and life cycle of this putative novel species.
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spelling pubmed-73975112020-09-01 Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus Luu, Lisa Bown, Kevin J. Palomar, Ana M. Kazimírová, Mária Bell-Sakyi, Lesley Ticks Tick Borne Dis Article Trypanosomes have long been recognised as being amongst the most important protozoan parasites of vertebrates, from both medical and veterinary perspectives. Whilst numerous insect species have been identified as vectors, the role of ticks is less well understood. Here we report the isolation and partial molecular characterisation of a novel trypanosome from questing Ixodes ricinus ticks collected in Slovakia. The trypanosome was isolated in tick cell culture and then partially characterised by microscopy and amplification of fragments of the 18S rRNA and 24Sα rDNA genes. Analysis of the resultant sequences suggests that the trypanosome designated as Trypanosoma sp. Bratislava1 may be a new species closely related to several species or strains of trypanosomes isolated from, or detected in, ticks in South America and Asia, and to Trypanosoma caninum isolated from dogs in Brazil. This study highlights the potential involvement of ixodid ticks in the epidemiology of trypanosomes, as well as the use of tick cell lines for isolation of such tick-borne protozoa. Further studies are required to investigate the epidemiology, transmission and life cycle of this putative novel species. Elsevier 2020-09 /pmc/articles/PMC7397511/ /pubmed/32723658 http://dx.doi.org/10.1016/j.ttbdis.2020.101501 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Luu, Lisa
Bown, Kevin J.
Palomar, Ana M.
Kazimírová, Mária
Bell-Sakyi, Lesley
Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus
title Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus
title_full Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus
title_fullStr Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus
title_full_unstemmed Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus
title_short Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus
title_sort isolation and partial characterisation of a novel trypanosoma from the tick ixodes ricinus
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397511/
https://www.ncbi.nlm.nih.gov/pubmed/32723658
http://dx.doi.org/10.1016/j.ttbdis.2020.101501
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