Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus
Trypanosomes have long been recognised as being amongst the most important protozoan parasites of vertebrates, from both medical and veterinary perspectives. Whilst numerous insect species have been identified as vectors, the role of ticks is less well understood. Here we report the isolation and pa...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Elsevier
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397511/ https://www.ncbi.nlm.nih.gov/pubmed/32723658 http://dx.doi.org/10.1016/j.ttbdis.2020.101501 |
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author | Luu, Lisa Bown, Kevin J. Palomar, Ana M. Kazimírová, Mária Bell-Sakyi, Lesley |
author_facet | Luu, Lisa Bown, Kevin J. Palomar, Ana M. Kazimírová, Mária Bell-Sakyi, Lesley |
author_sort | Luu, Lisa |
collection | PubMed |
description | Trypanosomes have long been recognised as being amongst the most important protozoan parasites of vertebrates, from both medical and veterinary perspectives. Whilst numerous insect species have been identified as vectors, the role of ticks is less well understood. Here we report the isolation and partial molecular characterisation of a novel trypanosome from questing Ixodes ricinus ticks collected in Slovakia. The trypanosome was isolated in tick cell culture and then partially characterised by microscopy and amplification of fragments of the 18S rRNA and 24Sα rDNA genes. Analysis of the resultant sequences suggests that the trypanosome designated as Trypanosoma sp. Bratislava1 may be a new species closely related to several species or strains of trypanosomes isolated from, or detected in, ticks in South America and Asia, and to Trypanosoma caninum isolated from dogs in Brazil. This study highlights the potential involvement of ixodid ticks in the epidemiology of trypanosomes, as well as the use of tick cell lines for isolation of such tick-borne protozoa. Further studies are required to investigate the epidemiology, transmission and life cycle of this putative novel species. |
format | Online Article Text |
id | pubmed-7397511 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Elsevier |
record_format | MEDLINE/PubMed |
spelling | pubmed-73975112020-09-01 Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus Luu, Lisa Bown, Kevin J. Palomar, Ana M. Kazimírová, Mária Bell-Sakyi, Lesley Ticks Tick Borne Dis Article Trypanosomes have long been recognised as being amongst the most important protozoan parasites of vertebrates, from both medical and veterinary perspectives. Whilst numerous insect species have been identified as vectors, the role of ticks is less well understood. Here we report the isolation and partial molecular characterisation of a novel trypanosome from questing Ixodes ricinus ticks collected in Slovakia. The trypanosome was isolated in tick cell culture and then partially characterised by microscopy and amplification of fragments of the 18S rRNA and 24Sα rDNA genes. Analysis of the resultant sequences suggests that the trypanosome designated as Trypanosoma sp. Bratislava1 may be a new species closely related to several species or strains of trypanosomes isolated from, or detected in, ticks in South America and Asia, and to Trypanosoma caninum isolated from dogs in Brazil. This study highlights the potential involvement of ixodid ticks in the epidemiology of trypanosomes, as well as the use of tick cell lines for isolation of such tick-borne protozoa. Further studies are required to investigate the epidemiology, transmission and life cycle of this putative novel species. Elsevier 2020-09 /pmc/articles/PMC7397511/ /pubmed/32723658 http://dx.doi.org/10.1016/j.ttbdis.2020.101501 Text en © 2020 The Authors http://creativecommons.org/licenses/by/4.0/ This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Luu, Lisa Bown, Kevin J. Palomar, Ana M. Kazimírová, Mária Bell-Sakyi, Lesley Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus |
title | Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus |
title_full | Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus |
title_fullStr | Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus |
title_full_unstemmed | Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus |
title_short | Isolation and partial characterisation of a novel Trypanosoma from the tick Ixodes ricinus |
title_sort | isolation and partial characterisation of a novel trypanosoma from the tick ixodes ricinus |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7397511/ https://www.ncbi.nlm.nih.gov/pubmed/32723658 http://dx.doi.org/10.1016/j.ttbdis.2020.101501 |
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