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High Ambient Temperature Accelerates Leaf Senescence via PHYTOCHROME-INTERACTING FACTOR 4 and 5 in Arabidopsis

Leaf senescence is a developmental process by which a plant actively remobilizes nutrients from aged and photosynthetically inefficient leaves to young growing ones by disassembling organelles and degrading macromolecules. Senescence is accelerated by age and environmental stresses such as prolonged...

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Autores principales: Kim, Chanhee, Kim, Sun Ji, Jeong, Jinkil, Park, Eunae, Oh, Eunkyoo, Park, Youn-Il, Lim, Pyung Ok, Choi, Giltsu
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Korean Society for Molecular and Cellular Biology 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7398796/
https://www.ncbi.nlm.nih.gov/pubmed/32732458
http://dx.doi.org/10.14348/molcells.2020.0117
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author Kim, Chanhee
Kim, Sun Ji
Jeong, Jinkil
Park, Eunae
Oh, Eunkyoo
Park, Youn-Il
Lim, Pyung Ok
Choi, Giltsu
author_facet Kim, Chanhee
Kim, Sun Ji
Jeong, Jinkil
Park, Eunae
Oh, Eunkyoo
Park, Youn-Il
Lim, Pyung Ok
Choi, Giltsu
author_sort Kim, Chanhee
collection PubMed
description Leaf senescence is a developmental process by which a plant actively remobilizes nutrients from aged and photosynthetically inefficient leaves to young growing ones by disassembling organelles and degrading macromolecules. Senescence is accelerated by age and environmental stresses such as prolonged darkness. Phytochrome B (phyB) inhibits leaf senescence by inhibiting phytochrome-interacting factor 4 (PIF4) and PIF5 in prolonged darkness. However, it remains unknown whether phyB mediates the temperature signal that regulates leaf senescence. We found the light-activated form of phyB (Pfr) remains active at least four days after a transfer to darkness at 20°C but is inactivated more rapidly at 28°C. This faster inactivation of Pfr further increases PIF4 protein levels at the higher ambient temperature. In addition, PIF4 mRNA levels rise faster after the transfer to darkness at high ambient temperature via a mechanism that depends on ELF3 but not phyB. Increased PIF4 protein then binds to the ORE1 promoter and activates its expression together with ABA and ethylene signaling, accelerating leaf senescence at high ambient temperature. Our results support a role for the phy-PIF signaling module in integrating not only light signaling but also temperature signaling in the regulation of leaf senescence.
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spelling pubmed-73987962020-08-11 High Ambient Temperature Accelerates Leaf Senescence via PHYTOCHROME-INTERACTING FACTOR 4 and 5 in Arabidopsis Kim, Chanhee Kim, Sun Ji Jeong, Jinkil Park, Eunae Oh, Eunkyoo Park, Youn-Il Lim, Pyung Ok Choi, Giltsu Mol Cells Research Article Leaf senescence is a developmental process by which a plant actively remobilizes nutrients from aged and photosynthetically inefficient leaves to young growing ones by disassembling organelles and degrading macromolecules. Senescence is accelerated by age and environmental stresses such as prolonged darkness. Phytochrome B (phyB) inhibits leaf senescence by inhibiting phytochrome-interacting factor 4 (PIF4) and PIF5 in prolonged darkness. However, it remains unknown whether phyB mediates the temperature signal that regulates leaf senescence. We found the light-activated form of phyB (Pfr) remains active at least four days after a transfer to darkness at 20°C but is inactivated more rapidly at 28°C. This faster inactivation of Pfr further increases PIF4 protein levels at the higher ambient temperature. In addition, PIF4 mRNA levels rise faster after the transfer to darkness at high ambient temperature via a mechanism that depends on ELF3 but not phyB. Increased PIF4 protein then binds to the ORE1 promoter and activates its expression together with ABA and ethylene signaling, accelerating leaf senescence at high ambient temperature. Our results support a role for the phy-PIF signaling module in integrating not only light signaling but also temperature signaling in the regulation of leaf senescence. Korean Society for Molecular and Cellular Biology 2020-07-31 2020-07-22 /pmc/articles/PMC7398796/ /pubmed/32732458 http://dx.doi.org/10.14348/molcells.2020.0117 Text en © The Korean Society for Molecular and Cellular Biology. All rights reserved. This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial-ShareAlike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/
spellingShingle Research Article
Kim, Chanhee
Kim, Sun Ji
Jeong, Jinkil
Park, Eunae
Oh, Eunkyoo
Park, Youn-Il
Lim, Pyung Ok
Choi, Giltsu
High Ambient Temperature Accelerates Leaf Senescence via PHYTOCHROME-INTERACTING FACTOR 4 and 5 in Arabidopsis
title High Ambient Temperature Accelerates Leaf Senescence via PHYTOCHROME-INTERACTING FACTOR 4 and 5 in Arabidopsis
title_full High Ambient Temperature Accelerates Leaf Senescence via PHYTOCHROME-INTERACTING FACTOR 4 and 5 in Arabidopsis
title_fullStr High Ambient Temperature Accelerates Leaf Senescence via PHYTOCHROME-INTERACTING FACTOR 4 and 5 in Arabidopsis
title_full_unstemmed High Ambient Temperature Accelerates Leaf Senescence via PHYTOCHROME-INTERACTING FACTOR 4 and 5 in Arabidopsis
title_short High Ambient Temperature Accelerates Leaf Senescence via PHYTOCHROME-INTERACTING FACTOR 4 and 5 in Arabidopsis
title_sort high ambient temperature accelerates leaf senescence via phytochrome-interacting factor 4 and 5 in arabidopsis
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7398796/
https://www.ncbi.nlm.nih.gov/pubmed/32732458
http://dx.doi.org/10.14348/molcells.2020.0117
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