Prevalence of murine astrovirus in laboratory animal facilities in Japan

To investigate the prevalence of murine astrovirus (MuAstV) in mice in laboratory animal facilities in Japan, a polymerase chain reaction (PCR) test targeting the RNA-dependent RNA polymerase (RdRP) gene was performed on the cecum contents of 1,212 mice (1,183 immunocompetent mice and 29 immunodefic...

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Detalles Bibliográficos
Autores principales: MORITA, Hanako, YASUDA, Masahiko, YAMAMOTO, Masafumi, UCHIDA, Ritsuki, TANAKA, Mai, ISHIDA, Tomoko, HAYASHIMOTO, Nobuhito
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Japanese Society of Veterinary Science 2020
Materias:
Laboratory Animal Science
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399306/
https://www.ncbi.nlm.nih.gov/pubmed/32418936
http://dx.doi.org/10.1292/jvms.20-0180
Descripción
Sumario:To investigate the prevalence of murine astrovirus (MuAstV) in mice in laboratory animal facilities in Japan, a polymerase chain reaction (PCR) test targeting the RNA-dependent RNA polymerase (RdRP) gene was performed on the cecum contents of 1,212 mice (1,183 immunocompetent mice and 29 immunodeficient mice) from 226 facilities. The results showed that 118 (52.2%) of the 226 facilities were positive for MuAstV. Out of the 1,212 mice, 424 (35.0%) were positive. No gross lesions were observed in any of the mice examined. A phylogenetic analysis for 15 selected strains revealed that 13 strains formed one cluster, while two were genetically distant from that cluster. These results suggest that multiple strains are prevalent in laboratory mice in Japan.

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