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Changes in plasma pH and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with Na(2)EDTA
This study was performed to confirm the alterations of blood and urine parameters in artificially induced hypocalcemic cows. For a 2 × 2 cross-over design, four non-pregnant, non-lactating Holstein Friesian cows (623 ± 63 kg) were utilized. Cows in the treatment and control group were infused with e...
Autores principales: | , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
The Japanese Society of Veterinary Science
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399321/ https://www.ncbi.nlm.nih.gov/pubmed/32448812 http://dx.doi.org/10.1292/jvms.20-0048 |
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author | RO, Younghye CHOI, Woojae PARK, Junho CHOE, Eunhui KIM, Danil |
author_facet | RO, Younghye CHOI, Woojae PARK, Junho CHOE, Eunhui KIM, Danil |
author_sort | RO, Younghye |
collection | PubMed |
description | This study was performed to confirm the alterations of blood and urine parameters in artificially induced hypocalcemic cows. For a 2 × 2 cross-over design, four non-pregnant, non-lactating Holstein Friesian cows (623 ± 63 kg) were utilized. Cows in the treatment and control group were infused with ethylenediaminetetraacetic acid (Na(2)EDTA) solution and normal saline through an intravenous catheter for 3 hr, respectively. Laboratory analyses included complete blood cell count, plasma chemistry, blood gas analysis and urine chemistry. During the hypocalcemic period, abnormal signs were not observed clinically, hematologically nor biochemically either in groups. But, plasma calcium and magnesium concentrations continued to decrease throughout Na(2)EDTA infusion, and significant group differences (P<0.05 or P<0.001) were detected until 5 hr after the initiation of infusion. Urinary excretions of these minerals were significantly reduced compared to the control group by 6 hr (Ca, P<0.05; Mg, P<0.001). Moreover, there is a significant group difference in the change in plasma pH at 1 hr after Na(2)EDTA infusion (P<0.05) and maintained a decreased level until 6 hr. Consequently, the blood pH was diminished simultaneously with hypocalcemia and hypomagnesemia induction in cows infused with Na(2)EDTA. This phenomenon may be one of the mechanisms to recover normocalcemia including maximizing the effect of parathyroid hormone, however, further studies are needed to elucidate the mechanism to alter the blood pH in hypocalcemia. |
format | Online Article Text |
id | pubmed-7399321 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | The Japanese Society of Veterinary Science |
record_format | MEDLINE/PubMed |
spelling | pubmed-73993212020-08-06 Changes in plasma pH and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with Na(2)EDTA RO, Younghye CHOI, Woojae PARK, Junho CHOE, Eunhui KIM, Danil J Vet Med Sci Internal Medicine This study was performed to confirm the alterations of blood and urine parameters in artificially induced hypocalcemic cows. For a 2 × 2 cross-over design, four non-pregnant, non-lactating Holstein Friesian cows (623 ± 63 kg) were utilized. Cows in the treatment and control group were infused with ethylenediaminetetraacetic acid (Na(2)EDTA) solution and normal saline through an intravenous catheter for 3 hr, respectively. Laboratory analyses included complete blood cell count, plasma chemistry, blood gas analysis and urine chemistry. During the hypocalcemic period, abnormal signs were not observed clinically, hematologically nor biochemically either in groups. But, plasma calcium and magnesium concentrations continued to decrease throughout Na(2)EDTA infusion, and significant group differences (P<0.05 or P<0.001) were detected until 5 hr after the initiation of infusion. Urinary excretions of these minerals were significantly reduced compared to the control group by 6 hr (Ca, P<0.05; Mg, P<0.001). Moreover, there is a significant group difference in the change in plasma pH at 1 hr after Na(2)EDTA infusion (P<0.05) and maintained a decreased level until 6 hr. Consequently, the blood pH was diminished simultaneously with hypocalcemia and hypomagnesemia induction in cows infused with Na(2)EDTA. This phenomenon may be one of the mechanisms to recover normocalcemia including maximizing the effect of parathyroid hormone, however, further studies are needed to elucidate the mechanism to alter the blood pH in hypocalcemia. The Japanese Society of Veterinary Science 2020-05-25 2020-07 /pmc/articles/PMC7399321/ /pubmed/32448812 http://dx.doi.org/10.1292/jvms.20-0048 Text en ©2020 The Japanese Society of Veterinary Science This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial No Derivatives (by-nc-nd) License. (CC-BY-NC-ND 4.0: https://creativecommons.org/licenses/by-nc-nd/4.0/) |
spellingShingle | Internal Medicine RO, Younghye CHOI, Woojae PARK, Junho CHOE, Eunhui KIM, Danil Changes in plasma pH and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with Na(2)EDTA |
title | Changes in plasma pH and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with Na(2)EDTA |
title_full | Changes in plasma pH and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with Na(2)EDTA |
title_fullStr | Changes in plasma pH and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with Na(2)EDTA |
title_full_unstemmed | Changes in plasma pH and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with Na(2)EDTA |
title_short | Changes in plasma pH and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with Na(2)EDTA |
title_sort | changes in plasma ph and blood and urinary macromineral concentrations in experimentally induced hypocalcemic cows with na(2)edta |
topic | Internal Medicine |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399321/ https://www.ncbi.nlm.nih.gov/pubmed/32448812 http://dx.doi.org/10.1292/jvms.20-0048 |
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