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The Effect of Chronic Mild Stress and Venlafaxine on the Expression and Methylation Levels of Genes Involved in the Tryptophan Catabolites Pathway in the Blood and Brain Structures of Rats

A growing body of evidence suggests that depression may be associated with impairment of the tryptophan catabolites (TRYCATs) pathway. The present study investigated the effects of the chronic administration of venlafaxine on the expression and methylation status of Katl, Tph1/2, Ido1, Kmo and Kynu...

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Autores principales: Wigner, Paulina, Synowiec, Ewelina, Jóźwiak, Paweł, Czarny, Piotr, Bijak, Michał, Białek, Katarzyna, Szemraj, Janusz, Gruca, Piotr, Papp, Mariusz, Śliwiński, Tomasz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Springer US 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399689/
https://www.ncbi.nlm.nih.gov/pubmed/32406039
http://dx.doi.org/10.1007/s12031-020-01563-2
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author Wigner, Paulina
Synowiec, Ewelina
Jóźwiak, Paweł
Czarny, Piotr
Bijak, Michał
Białek, Katarzyna
Szemraj, Janusz
Gruca, Piotr
Papp, Mariusz
Śliwiński, Tomasz
author_facet Wigner, Paulina
Synowiec, Ewelina
Jóźwiak, Paweł
Czarny, Piotr
Bijak, Michał
Białek, Katarzyna
Szemraj, Janusz
Gruca, Piotr
Papp, Mariusz
Śliwiński, Tomasz
author_sort Wigner, Paulina
collection PubMed
description A growing body of evidence suggests that depression may be associated with impairment of the tryptophan catabolites (TRYCATs) pathway. The present study investigated the effects of the chronic administration of venlafaxine on the expression and methylation status of Katl, Tph1/2, Ido1, Kmo and Kynu in the brain and blood of rats exposed to the CMS model of depression. The rats were subjected to the CMS procedure for 2 or 7 weeks and administered venlafaxine (10 mg/kg/day, IP) for 5 weeks. mRNA and protein expression and the methylation status of gene promoters in PBMCs and six brain structures were evaluated and analysed using the TaqMan Gene Expression Assay and Western blotting, and methylation-sensitive high-resolution melting (MS-HRM), respectively. We found that the CMS procedure increased KatI expression in the midbrain and KatII expression in the midbrain and the amygdala, while venlafaxine administration decreased KatII expression in the hypothalamus and the cerebral cortex. The methylation status of the Tph1 and Kmo promoters in peripheral blood mononuclear cells (PBMCs) was significantly increased in the stressed group after antidepressant therapy. The protein levels of Tph1 and Ido1 were decreased following venlafaxine administration. Our results confirmed that CMS and venlafaxine modulate the expression levels and methylation status of genes involved in the TRYCATs pathway. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12031-020-01563-2) contains supplementary material, which is available to authorized users.
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spelling pubmed-73996892020-08-13 The Effect of Chronic Mild Stress and Venlafaxine on the Expression and Methylation Levels of Genes Involved in the Tryptophan Catabolites Pathway in the Blood and Brain Structures of Rats Wigner, Paulina Synowiec, Ewelina Jóźwiak, Paweł Czarny, Piotr Bijak, Michał Białek, Katarzyna Szemraj, Janusz Gruca, Piotr Papp, Mariusz Śliwiński, Tomasz J Mol Neurosci Article A growing body of evidence suggests that depression may be associated with impairment of the tryptophan catabolites (TRYCATs) pathway. The present study investigated the effects of the chronic administration of venlafaxine on the expression and methylation status of Katl, Tph1/2, Ido1, Kmo and Kynu in the brain and blood of rats exposed to the CMS model of depression. The rats were subjected to the CMS procedure for 2 or 7 weeks and administered venlafaxine (10 mg/kg/day, IP) for 5 weeks. mRNA and protein expression and the methylation status of gene promoters in PBMCs and six brain structures were evaluated and analysed using the TaqMan Gene Expression Assay and Western blotting, and methylation-sensitive high-resolution melting (MS-HRM), respectively. We found that the CMS procedure increased KatI expression in the midbrain and KatII expression in the midbrain and the amygdala, while venlafaxine administration decreased KatII expression in the hypothalamus and the cerebral cortex. The methylation status of the Tph1 and Kmo promoters in peripheral blood mononuclear cells (PBMCs) was significantly increased in the stressed group after antidepressant therapy. The protein levels of Tph1 and Ido1 were decreased following venlafaxine administration. Our results confirmed that CMS and venlafaxine modulate the expression levels and methylation status of genes involved in the TRYCATs pathway. ELECTRONIC SUPPLEMENTARY MATERIAL: The online version of this article (10.1007/s12031-020-01563-2) contains supplementary material, which is available to authorized users. Springer US 2020-05-13 2020 /pmc/articles/PMC7399689/ /pubmed/32406039 http://dx.doi.org/10.1007/s12031-020-01563-2 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
spellingShingle Article
Wigner, Paulina
Synowiec, Ewelina
Jóźwiak, Paweł
Czarny, Piotr
Bijak, Michał
Białek, Katarzyna
Szemraj, Janusz
Gruca, Piotr
Papp, Mariusz
Śliwiński, Tomasz
The Effect of Chronic Mild Stress and Venlafaxine on the Expression and Methylation Levels of Genes Involved in the Tryptophan Catabolites Pathway in the Blood and Brain Structures of Rats
title The Effect of Chronic Mild Stress and Venlafaxine on the Expression and Methylation Levels of Genes Involved in the Tryptophan Catabolites Pathway in the Blood and Brain Structures of Rats
title_full The Effect of Chronic Mild Stress and Venlafaxine on the Expression and Methylation Levels of Genes Involved in the Tryptophan Catabolites Pathway in the Blood and Brain Structures of Rats
title_fullStr The Effect of Chronic Mild Stress and Venlafaxine on the Expression and Methylation Levels of Genes Involved in the Tryptophan Catabolites Pathway in the Blood and Brain Structures of Rats
title_full_unstemmed The Effect of Chronic Mild Stress and Venlafaxine on the Expression and Methylation Levels of Genes Involved in the Tryptophan Catabolites Pathway in the Blood and Brain Structures of Rats
title_short The Effect of Chronic Mild Stress and Venlafaxine on the Expression and Methylation Levels of Genes Involved in the Tryptophan Catabolites Pathway in the Blood and Brain Structures of Rats
title_sort effect of chronic mild stress and venlafaxine on the expression and methylation levels of genes involved in the tryptophan catabolites pathway in the blood and brain structures of rats
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399689/
https://www.ncbi.nlm.nih.gov/pubmed/32406039
http://dx.doi.org/10.1007/s12031-020-01563-2
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