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Polycystic Kidney Disease Ryanodine Receptor Domain (PKDRR) Proteins in Oomycetes

In eukaryotes, two sources of Ca(2+) are accessed to allow rapid changes in the cytosolic levels of this second messenger: the extracellular medium and intracellular Ca(2+) stores, such as the endoplasmic reticulum. One class of channel that permits Ca(2+) entry is the transient receptor potential (...

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Autores principales: Zheng, Limian, Prestwich, Barbara Doyle, Harrison, Patrick T., Mackrill, John J.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399828/
https://www.ncbi.nlm.nih.gov/pubmed/32708691
http://dx.doi.org/10.3390/pathogens9070577
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author Zheng, Limian
Prestwich, Barbara Doyle
Harrison, Patrick T.
Mackrill, John J.
author_facet Zheng, Limian
Prestwich, Barbara Doyle
Harrison, Patrick T.
Mackrill, John J.
author_sort Zheng, Limian
collection PubMed
description In eukaryotes, two sources of Ca(2+) are accessed to allow rapid changes in the cytosolic levels of this second messenger: the extracellular medium and intracellular Ca(2+) stores, such as the endoplasmic reticulum. One class of channel that permits Ca(2+) entry is the transient receptor potential (TRP) superfamily, including the polycystic kidney disease (PKD) proteins, or polycystins. Channels that release Ca(2+) from intracellular stores include the inositol 1,4,5-trisphosphate/ryanodine receptor (ITPR/RyR) superfamily. Here, we characterise a family of proteins that are only encoded by oomycete genomes, that we have named PKDRR, since they share domains with both PKD and RyR channels. We provide evidence that these proteins belong to the TRP superfamily and are distinct from the ITPR/RyR superfamily in terms of their evolutionary relationships, protein domain architectures and predicted ion channel structures. We also demonstrate that a hypothetical PKDRR protein from Phytophthora infestans is produced by this organism, is located in the cell-surface membrane and forms multimeric protein complexes. Efforts to functionally characterise this protein in a heterologous expression system were unsuccessful but support a cell-surface localisation. These PKDRR proteins represent potential targets for the development of new “fungicides”, since they are of a distinctive structure that is only found in oomycetes and not in any other cellular organisms.
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spelling pubmed-73998282020-08-17 Polycystic Kidney Disease Ryanodine Receptor Domain (PKDRR) Proteins in Oomycetes Zheng, Limian Prestwich, Barbara Doyle Harrison, Patrick T. Mackrill, John J. Pathogens Article In eukaryotes, two sources of Ca(2+) are accessed to allow rapid changes in the cytosolic levels of this second messenger: the extracellular medium and intracellular Ca(2+) stores, such as the endoplasmic reticulum. One class of channel that permits Ca(2+) entry is the transient receptor potential (TRP) superfamily, including the polycystic kidney disease (PKD) proteins, or polycystins. Channels that release Ca(2+) from intracellular stores include the inositol 1,4,5-trisphosphate/ryanodine receptor (ITPR/RyR) superfamily. Here, we characterise a family of proteins that are only encoded by oomycete genomes, that we have named PKDRR, since they share domains with both PKD and RyR channels. We provide evidence that these proteins belong to the TRP superfamily and are distinct from the ITPR/RyR superfamily in terms of their evolutionary relationships, protein domain architectures and predicted ion channel structures. We also demonstrate that a hypothetical PKDRR protein from Phytophthora infestans is produced by this organism, is located in the cell-surface membrane and forms multimeric protein complexes. Efforts to functionally characterise this protein in a heterologous expression system were unsuccessful but support a cell-surface localisation. These PKDRR proteins represent potential targets for the development of new “fungicides”, since they are of a distinctive structure that is only found in oomycetes and not in any other cellular organisms. MDPI 2020-07-16 /pmc/articles/PMC7399828/ /pubmed/32708691 http://dx.doi.org/10.3390/pathogens9070577 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zheng, Limian
Prestwich, Barbara Doyle
Harrison, Patrick T.
Mackrill, John J.
Polycystic Kidney Disease Ryanodine Receptor Domain (PKDRR) Proteins in Oomycetes
title Polycystic Kidney Disease Ryanodine Receptor Domain (PKDRR) Proteins in Oomycetes
title_full Polycystic Kidney Disease Ryanodine Receptor Domain (PKDRR) Proteins in Oomycetes
title_fullStr Polycystic Kidney Disease Ryanodine Receptor Domain (PKDRR) Proteins in Oomycetes
title_full_unstemmed Polycystic Kidney Disease Ryanodine Receptor Domain (PKDRR) Proteins in Oomycetes
title_short Polycystic Kidney Disease Ryanodine Receptor Domain (PKDRR) Proteins in Oomycetes
title_sort polycystic kidney disease ryanodine receptor domain (pkdrr) proteins in oomycetes
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7399828/
https://www.ncbi.nlm.nih.gov/pubmed/32708691
http://dx.doi.org/10.3390/pathogens9070577
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