Cargando…

A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach

Modulation of the gut microbiome as a means to improve human health has recently gained increasing interest. In this study, it was investigated whether cRG-I, a carrot-derived pectic polysaccharide, enriched in rhamnogalacturonan-I (RG-I) classifies as a potential prebiotic ingredient using novel in...

Descripción completa

Detalles Bibliográficos
Autores principales: Van den Abbeele, Pieter, Verstrepen, Lynn, Ghyselinck, Jonas, Albers, Ruud, Marzorati, Massimo, Mercenier, Annick
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400138/
https://www.ncbi.nlm.nih.gov/pubmed/32610452
http://dx.doi.org/10.3390/nu12071917
_version_ 1783566295574249472
author Van den Abbeele, Pieter
Verstrepen, Lynn
Ghyselinck, Jonas
Albers, Ruud
Marzorati, Massimo
Mercenier, Annick
author_facet Van den Abbeele, Pieter
Verstrepen, Lynn
Ghyselinck, Jonas
Albers, Ruud
Marzorati, Massimo
Mercenier, Annick
author_sort Van den Abbeele, Pieter
collection PubMed
description Modulation of the gut microbiome as a means to improve human health has recently gained increasing interest. In this study, it was investigated whether cRG-I, a carrot-derived pectic polysaccharide, enriched in rhamnogalacturonan-I (RG-I) classifies as a potential prebiotic ingredient using novel in vitro models. First, digestion methods involving α-amylase/brush border enzymes demonstrated the non-digestibility of cRG-I by host-derived enzymes versus digestible (starch/maltose) and non-digestible controls (inulin). Then, a recently developed short-term (48 h) colonic incubation strategy was applied and revealed that cRG-I fermentation increased levels of health-promoting short-chain fatty acids (SCFA; mainly acetate and propionate) and lactate comparable but not identical to the reference prebiotic inulin. Upon upgrading this fermentation model by inclusion of a simulated mucosal environment while applying quantitative 16S-targeted Illumina sequencing, cRG-I was additionally shown to specifically stimulate operational taxonomic units (OTUs) related to health-associated species such as Bifidobacterium longum, Bifidobacterium adolescentis, Bacteroides dorei, Bacteroides ovatus, Roseburia hominis, Faecalibacterium prausnitzii, and Eubacterium hallii. Finally, in a novel model to assess host–microbe interactions (Caco-2/peripheral blood mononuclear cells (PBMC) co-culture) fermented cRG-I increased barrier integrity while decreasing markers for inflammation. In conclusion, by using novel in vitro models, cRG-I was identified as a promising prebiotic candidate to proceed to clinical studies.
format Online
Article
Text
id pubmed-7400138
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74001382020-08-23 A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach Van den Abbeele, Pieter Verstrepen, Lynn Ghyselinck, Jonas Albers, Ruud Marzorati, Massimo Mercenier, Annick Nutrients Article Modulation of the gut microbiome as a means to improve human health has recently gained increasing interest. In this study, it was investigated whether cRG-I, a carrot-derived pectic polysaccharide, enriched in rhamnogalacturonan-I (RG-I) classifies as a potential prebiotic ingredient using novel in vitro models. First, digestion methods involving α-amylase/brush border enzymes demonstrated the non-digestibility of cRG-I by host-derived enzymes versus digestible (starch/maltose) and non-digestible controls (inulin). Then, a recently developed short-term (48 h) colonic incubation strategy was applied and revealed that cRG-I fermentation increased levels of health-promoting short-chain fatty acids (SCFA; mainly acetate and propionate) and lactate comparable but not identical to the reference prebiotic inulin. Upon upgrading this fermentation model by inclusion of a simulated mucosal environment while applying quantitative 16S-targeted Illumina sequencing, cRG-I was additionally shown to specifically stimulate operational taxonomic units (OTUs) related to health-associated species such as Bifidobacterium longum, Bifidobacterium adolescentis, Bacteroides dorei, Bacteroides ovatus, Roseburia hominis, Faecalibacterium prausnitzii, and Eubacterium hallii. Finally, in a novel model to assess host–microbe interactions (Caco-2/peripheral blood mononuclear cells (PBMC) co-culture) fermented cRG-I increased barrier integrity while decreasing markers for inflammation. In conclusion, by using novel in vitro models, cRG-I was identified as a promising prebiotic candidate to proceed to clinical studies. MDPI 2020-06-29 /pmc/articles/PMC7400138/ /pubmed/32610452 http://dx.doi.org/10.3390/nu12071917 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Van den Abbeele, Pieter
Verstrepen, Lynn
Ghyselinck, Jonas
Albers, Ruud
Marzorati, Massimo
Mercenier, Annick
A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach
title A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach
title_full A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach
title_fullStr A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach
title_full_unstemmed A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach
title_short A Novel Non-Digestible, Carrot-Derived Polysaccharide (cRG-I) Selectively Modulates the Human Gut Microbiota while Promoting Gut Barrier Integrity: An Integrated In Vitro Approach
title_sort novel non-digestible, carrot-derived polysaccharide (crg-i) selectively modulates the human gut microbiota while promoting gut barrier integrity: an integrated in vitro approach
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400138/
https://www.ncbi.nlm.nih.gov/pubmed/32610452
http://dx.doi.org/10.3390/nu12071917
work_keys_str_mv AT vandenabbeelepieter anovelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach
AT verstrepenlynn anovelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach
AT ghyselinckjonas anovelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach
AT albersruud anovelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach
AT marzoratimassimo anovelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach
AT mercenierannick anovelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach
AT vandenabbeelepieter novelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach
AT verstrepenlynn novelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach
AT ghyselinckjonas novelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach
AT albersruud novelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach
AT marzoratimassimo novelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach
AT mercenierannick novelnondigestiblecarrotderivedpolysaccharidecrgiselectivelymodulatesthehumangutmicrobiotawhilepromotinggutbarrierintegrityanintegratedinvitroapproach