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Animal Models for Studying Stone Disease
Animals have stone disease too. There are several animal models for the research of human stone disease. Rodents are the most frequently used for stone research, although they are not prone to forming crystals in the kidneys. Ethylene glycol (EG), sodium oxalate and l-hydroxyproline are common litho...
Autores principales: | , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400259/ https://www.ncbi.nlm.nih.gov/pubmed/32708380 http://dx.doi.org/10.3390/diagnostics10070490 |
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author | Chen, Szu-Ju Chiu, Kun-Yuan Chen, Huey-Yi Lin, Wei-Yong Chen, Yung-Hsiang Chen, Wen-Chi |
author_facet | Chen, Szu-Ju Chiu, Kun-Yuan Chen, Huey-Yi Lin, Wei-Yong Chen, Yung-Hsiang Chen, Wen-Chi |
author_sort | Chen, Szu-Ju |
collection | PubMed |
description | Animals have stone disease too. There are several animal models for the research of human stone disease. Rodents are the most frequently used for stone research, although they are not prone to forming crystals in the kidneys. Ethylene glycol (EG), sodium oxalate and l-hydroxyproline are common lithogenic agents. Dogs and pigs were also reported as a study animal for stone disease. However, the breeding costs and body size are too high. The most-used genetic study animal for stone disease was the mouse, but it was high-cost. Calcium oxalate (CaOx) crystals can also be light microscopically observed in the Malphigian tubules of Drosophila melanogaster, induced by adding EG to the food. Genetic studies of flies can be done by cross-breeding, and this has a lower cost than using mice. The fly model also has several advantages, including minimal breeding equipment, the fact that it is easier to reach larger numbers in a short time with flies, that crystals can be observed under microscopy, and that they allow genetic study. We suggest the fly will be an ideal animal model for stone research in the future. |
format | Online Article Text |
id | pubmed-7400259 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74002592020-08-23 Animal Models for Studying Stone Disease Chen, Szu-Ju Chiu, Kun-Yuan Chen, Huey-Yi Lin, Wei-Yong Chen, Yung-Hsiang Chen, Wen-Chi Diagnostics (Basel) Review Animals have stone disease too. There are several animal models for the research of human stone disease. Rodents are the most frequently used for stone research, although they are not prone to forming crystals in the kidneys. Ethylene glycol (EG), sodium oxalate and l-hydroxyproline are common lithogenic agents. Dogs and pigs were also reported as a study animal for stone disease. However, the breeding costs and body size are too high. The most-used genetic study animal for stone disease was the mouse, but it was high-cost. Calcium oxalate (CaOx) crystals can also be light microscopically observed in the Malphigian tubules of Drosophila melanogaster, induced by adding EG to the food. Genetic studies of flies can be done by cross-breeding, and this has a lower cost than using mice. The fly model also has several advantages, including minimal breeding equipment, the fact that it is easier to reach larger numbers in a short time with flies, that crystals can be observed under microscopy, and that they allow genetic study. We suggest the fly will be an ideal animal model for stone research in the future. MDPI 2020-07-18 /pmc/articles/PMC7400259/ /pubmed/32708380 http://dx.doi.org/10.3390/diagnostics10070490 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Review Chen, Szu-Ju Chiu, Kun-Yuan Chen, Huey-Yi Lin, Wei-Yong Chen, Yung-Hsiang Chen, Wen-Chi Animal Models for Studying Stone Disease |
title | Animal Models for Studying Stone Disease |
title_full | Animal Models for Studying Stone Disease |
title_fullStr | Animal Models for Studying Stone Disease |
title_full_unstemmed | Animal Models for Studying Stone Disease |
title_short | Animal Models for Studying Stone Disease |
title_sort | animal models for studying stone disease |
topic | Review |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400259/ https://www.ncbi.nlm.nih.gov/pubmed/32708380 http://dx.doi.org/10.3390/diagnostics10070490 |
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