Cargando…

Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae

Mortalities of bivalve larvae and spat linked with Vibrio spp. infection have been described in hatcheries since 1959, causing potential development of resistant bacteria. A reliable and sustainable solution to this problem is yet to be developed. Potential treatment of bacterial infection with bact...

Descripción completa

Detalles Bibliográficos
Autores principales: Le, Tuan Son, Southgate, Paul C., O’Connor, Wayne, Vu, Sang V., Kurtböke, D. İpek
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400271/
https://www.ncbi.nlm.nih.gov/pubmed/32708768
http://dx.doi.org/10.3390/antibiotics9070415
_version_ 1783566324950106112
author Le, Tuan Son
Southgate, Paul C.
O’Connor, Wayne
Vu, Sang V.
Kurtböke, D. İpek
author_facet Le, Tuan Son
Southgate, Paul C.
O’Connor, Wayne
Vu, Sang V.
Kurtböke, D. İpek
author_sort Le, Tuan Son
collection PubMed
description Mortalities of bivalve larvae and spat linked with Vibrio spp. infection have been described in hatcheries since 1959, causing potential development of resistant bacteria. A reliable and sustainable solution to this problem is yet to be developed. Potential treatment of bacterial infection with bacteriophages is gaining interest in aquaculture as a more sustainable option for managing Vibrio spp. infection. This study assessed the effectiveness of bacteriophages (Φ-5, Φ-6, and Φ-7) against pathogenic Vibrio isolates (USC-26004 and USC-26005). These phage isolates were found to belong to the Myoviridae viral family. A total of 212 ORFs of Φ-5 were identified and annotated. The genome of this phage contained putative thymidine kinase and lysin enzyme. During infections with phages, the OD values of the isolates USC-26005 and USC-26004 remained stable at a much lower reading compared to the control after 9 h of incubation. Mortality rate of oyster (Saccostrea glomerata) larvae was 28.2 ± 3.5% in the bacteriophage treatment group, compared to 77.9 ± 9.1% in the bacterial treatment group after 24 h incubation. Findings of this study indicate that lytic phages might be utilized as potential bio-control agents of luminescent bacterial disease in oyster hatcheries.
format Online
Article
Text
id pubmed-7400271
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74002712020-08-23 Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae Le, Tuan Son Southgate, Paul C. O’Connor, Wayne Vu, Sang V. Kurtböke, D. İpek Antibiotics (Basel) Article Mortalities of bivalve larvae and spat linked with Vibrio spp. infection have been described in hatcheries since 1959, causing potential development of resistant bacteria. A reliable and sustainable solution to this problem is yet to be developed. Potential treatment of bacterial infection with bacteriophages is gaining interest in aquaculture as a more sustainable option for managing Vibrio spp. infection. This study assessed the effectiveness of bacteriophages (Φ-5, Φ-6, and Φ-7) against pathogenic Vibrio isolates (USC-26004 and USC-26005). These phage isolates were found to belong to the Myoviridae viral family. A total of 212 ORFs of Φ-5 were identified and annotated. The genome of this phage contained putative thymidine kinase and lysin enzyme. During infections with phages, the OD values of the isolates USC-26005 and USC-26004 remained stable at a much lower reading compared to the control after 9 h of incubation. Mortality rate of oyster (Saccostrea glomerata) larvae was 28.2 ± 3.5% in the bacteriophage treatment group, compared to 77.9 ± 9.1% in the bacterial treatment group after 24 h incubation. Findings of this study indicate that lytic phages might be utilized as potential bio-control agents of luminescent bacterial disease in oyster hatcheries. MDPI 2020-07-16 /pmc/articles/PMC7400271/ /pubmed/32708768 http://dx.doi.org/10.3390/antibiotics9070415 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Le, Tuan Son
Southgate, Paul C.
O’Connor, Wayne
Vu, Sang V.
Kurtböke, D. İpek
Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae
title Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae
title_full Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae
title_fullStr Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae
title_full_unstemmed Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae
title_short Application of Bacteriophages to Control Vibrio alginolyticus Contamination in Oyster (Saccostrea glomerata) Larvae
title_sort application of bacteriophages to control vibrio alginolyticus contamination in oyster (saccostrea glomerata) larvae
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400271/
https://www.ncbi.nlm.nih.gov/pubmed/32708768
http://dx.doi.org/10.3390/antibiotics9070415
work_keys_str_mv AT letuanson applicationofbacteriophagestocontrolvibrioalginolyticuscontaminationinoystersaccostreaglomeratalarvae
AT southgatepaulc applicationofbacteriophagestocontrolvibrioalginolyticuscontaminationinoystersaccostreaglomeratalarvae
AT oconnorwayne applicationofbacteriophagestocontrolvibrioalginolyticuscontaminationinoystersaccostreaglomeratalarvae
AT vusangv applicationofbacteriophagestocontrolvibrioalginolyticuscontaminationinoystersaccostreaglomeratalarvae
AT kurtbokedipek applicationofbacteriophagestocontrolvibrioalginolyticuscontaminationinoystersaccostreaglomeratalarvae