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Suppression of Rice Blast by Bacterial Strains Isolated from Cultivated Soda Saline-Sodic Soils

Rice blast caused by Magnaporthe oryzae is one of the most serious rice diseases worldwide. Biological control is gaining popularity as a promising method for the control of this disease; however, more effective microbial strains with strong adaptability in rice fields need to be identified. Here, w...

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Autores principales: Wei, Yi, Li, Lanhui, Hu, Wenjun, Ju, Huiyan, Zhang, Mingzhe, Qin, Qingming, Zhang, Shihong, Li, Guihua
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400292/
https://www.ncbi.nlm.nih.gov/pubmed/32708144
http://dx.doi.org/10.3390/ijerph17145248
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author Wei, Yi
Li, Lanhui
Hu, Wenjun
Ju, Huiyan
Zhang, Mingzhe
Qin, Qingming
Zhang, Shihong
Li, Guihua
author_facet Wei, Yi
Li, Lanhui
Hu, Wenjun
Ju, Huiyan
Zhang, Mingzhe
Qin, Qingming
Zhang, Shihong
Li, Guihua
author_sort Wei, Yi
collection PubMed
description Rice blast caused by Magnaporthe oryzae is one of the most serious rice diseases worldwide. Biological control is gaining popularity as a promising method for the control of this disease; however, more effective microbial strains with strong adaptability in rice fields need to be identified. Here, we report for the first time the successful identification of biocontrol bacterial strains from frozen soils of the soda saline-sodic land. We isolated 82 bacterial strains from rice fields in the western Songnen Plain of China, one of the three major soda saline soils in the world. Five of the isolated strains exhibited strong inhibition to M. oryzae growth. The potential strains were identified as Bacillus safensis JLS5, Pseudomonas koreensis JLS8, Pseudomonas saponiphila JLS10, Stenotrophomonas rhizophila JLS11 and Bacillus tequilensis JLS12, respectively, by 16s RNA gene sequence analysis. The antagonistic assay and the artificial inoculation tests showed that JLS5 and JLS12 could effectively inhibit conidial germination and pathogenicity of the rice blast fungus, both preventively and curatively. The suppression of pathogenicity was further confirmed by greenhouse experiments, showing the effectiveness of JLS5 and JLS12 as a potential biological control agents of M. oryzae. The potential application of these cold-tolerant strains for rice blast control in cold regions is discussed. Our data suggest that soda saline-sodic soils are a rich source for biocontrol strain isolation.
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spelling pubmed-74002922020-08-23 Suppression of Rice Blast by Bacterial Strains Isolated from Cultivated Soda Saline-Sodic Soils Wei, Yi Li, Lanhui Hu, Wenjun Ju, Huiyan Zhang, Mingzhe Qin, Qingming Zhang, Shihong Li, Guihua Int J Environ Res Public Health Article Rice blast caused by Magnaporthe oryzae is one of the most serious rice diseases worldwide. Biological control is gaining popularity as a promising method for the control of this disease; however, more effective microbial strains with strong adaptability in rice fields need to be identified. Here, we report for the first time the successful identification of biocontrol bacterial strains from frozen soils of the soda saline-sodic land. We isolated 82 bacterial strains from rice fields in the western Songnen Plain of China, one of the three major soda saline soils in the world. Five of the isolated strains exhibited strong inhibition to M. oryzae growth. The potential strains were identified as Bacillus safensis JLS5, Pseudomonas koreensis JLS8, Pseudomonas saponiphila JLS10, Stenotrophomonas rhizophila JLS11 and Bacillus tequilensis JLS12, respectively, by 16s RNA gene sequence analysis. The antagonistic assay and the artificial inoculation tests showed that JLS5 and JLS12 could effectively inhibit conidial germination and pathogenicity of the rice blast fungus, both preventively and curatively. The suppression of pathogenicity was further confirmed by greenhouse experiments, showing the effectiveness of JLS5 and JLS12 as a potential biological control agents of M. oryzae. The potential application of these cold-tolerant strains for rice blast control in cold regions is discussed. Our data suggest that soda saline-sodic soils are a rich source for biocontrol strain isolation. MDPI 2020-07-21 2020-07 /pmc/articles/PMC7400292/ /pubmed/32708144 http://dx.doi.org/10.3390/ijerph17145248 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wei, Yi
Li, Lanhui
Hu, Wenjun
Ju, Huiyan
Zhang, Mingzhe
Qin, Qingming
Zhang, Shihong
Li, Guihua
Suppression of Rice Blast by Bacterial Strains Isolated from Cultivated Soda Saline-Sodic Soils
title Suppression of Rice Blast by Bacterial Strains Isolated from Cultivated Soda Saline-Sodic Soils
title_full Suppression of Rice Blast by Bacterial Strains Isolated from Cultivated Soda Saline-Sodic Soils
title_fullStr Suppression of Rice Blast by Bacterial Strains Isolated from Cultivated Soda Saline-Sodic Soils
title_full_unstemmed Suppression of Rice Blast by Bacterial Strains Isolated from Cultivated Soda Saline-Sodic Soils
title_short Suppression of Rice Blast by Bacterial Strains Isolated from Cultivated Soda Saline-Sodic Soils
title_sort suppression of rice blast by bacterial strains isolated from cultivated soda saline-sodic soils
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400292/
https://www.ncbi.nlm.nih.gov/pubmed/32708144
http://dx.doi.org/10.3390/ijerph17145248
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