Cargando…

Mitochondrial Membrane Potential Predicts 4-Hour Sperm Motility

The evaluation of conventional and biofunctional sperm parameters is of fundamental importance for assessing male reproductive function. Among these, sperm motility is one of the most important parameters. Indeed, asthenozoospermia is a frequent cause of male infertility. Sperm motility depends on m...

Descripción completa

Detalles Bibliográficos
Autores principales: Alamo, Angela, De Luca, Claudia, Mongioì, Laura M., Barbagallo, Federica, Cannarella, Rossella, La Vignera, Sandro, Calogero, Aldo E., Condorelli, Rosita A.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400390/
https://www.ncbi.nlm.nih.gov/pubmed/32645820
http://dx.doi.org/10.3390/biomedicines8070196
_version_ 1783566352748904448
author Alamo, Angela
De Luca, Claudia
Mongioì, Laura M.
Barbagallo, Federica
Cannarella, Rossella
La Vignera, Sandro
Calogero, Aldo E.
Condorelli, Rosita A.
author_facet Alamo, Angela
De Luca, Claudia
Mongioì, Laura M.
Barbagallo, Federica
Cannarella, Rossella
La Vignera, Sandro
Calogero, Aldo E.
Condorelli, Rosita A.
author_sort Alamo, Angela
collection PubMed
description The evaluation of conventional and biofunctional sperm parameters is of fundamental importance for assessing male reproductive function. Among these, sperm motility is one of the most important parameters. Indeed, asthenozoospermia is a frequent cause of male infertility. Sperm motility depends on mitochondrial function and the measurement of mitochondrial membrane potential (MMP) better accounts for the function of this intracellular organelle. On the basis of these premises, the present study assessed whether the MMP predicts sperm motility at 4 h in patients with low or normal MMP. To accomplish this, 31 men were enrolled. Sperm analysis was conducted according to the WHO 2010 criteria. Particular attention was paid to the evaluation of MMP after liquefaction (T0) using JC-1 staining by flow cytometry. Sperm total and progressive motility were measured at T0 and after 4 h from seminal fluid collection (T4). Patients were divided into two groups based on their sperm mitochondrial function at T0. Group A (n = 18) was composed of men with normal mitochondrial function since they had a percentage of spermatozoa with low MMP (L-MMP) below the normal reference value of our laboratory (<36.5%). In contrast, group B (n = 13) was made up of men with impaired sperm mitochondrial function (L-MMP > 36.5%). Group A had a slight but not significant reduction in total and progressive sperm motility at T4 compared with the values recorded at T0. In contrast, patients in group B showed a significant decline in both total and progressive sperm motility at T4 compared with T0 (p < 0.05). The results of this study showed that worse mitochondrial function, assessed by staining with JC1, is associated with a significant decline in sperm motility over time. These findings may be of clinical relevance in programs of assisted reproduction techniques. Based on our knowledge, there is no other evidence in the literature that has shown this relationship in healthy men with low MMP of idiopathic etiology, but normozoospermics according to the WHO 2010 criteria.
format Online
Article
Text
id pubmed-7400390
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74003902020-08-23 Mitochondrial Membrane Potential Predicts 4-Hour Sperm Motility Alamo, Angela De Luca, Claudia Mongioì, Laura M. Barbagallo, Federica Cannarella, Rossella La Vignera, Sandro Calogero, Aldo E. Condorelli, Rosita A. Biomedicines Article The evaluation of conventional and biofunctional sperm parameters is of fundamental importance for assessing male reproductive function. Among these, sperm motility is one of the most important parameters. Indeed, asthenozoospermia is a frequent cause of male infertility. Sperm motility depends on mitochondrial function and the measurement of mitochondrial membrane potential (MMP) better accounts for the function of this intracellular organelle. On the basis of these premises, the present study assessed whether the MMP predicts sperm motility at 4 h in patients with low or normal MMP. To accomplish this, 31 men were enrolled. Sperm analysis was conducted according to the WHO 2010 criteria. Particular attention was paid to the evaluation of MMP after liquefaction (T0) using JC-1 staining by flow cytometry. Sperm total and progressive motility were measured at T0 and after 4 h from seminal fluid collection (T4). Patients were divided into two groups based on their sperm mitochondrial function at T0. Group A (n = 18) was composed of men with normal mitochondrial function since they had a percentage of spermatozoa with low MMP (L-MMP) below the normal reference value of our laboratory (<36.5%). In contrast, group B (n = 13) was made up of men with impaired sperm mitochondrial function (L-MMP > 36.5%). Group A had a slight but not significant reduction in total and progressive sperm motility at T4 compared with the values recorded at T0. In contrast, patients in group B showed a significant decline in both total and progressive sperm motility at T4 compared with T0 (p < 0.05). The results of this study showed that worse mitochondrial function, assessed by staining with JC1, is associated with a significant decline in sperm motility over time. These findings may be of clinical relevance in programs of assisted reproduction techniques. Based on our knowledge, there is no other evidence in the literature that has shown this relationship in healthy men with low MMP of idiopathic etiology, but normozoospermics according to the WHO 2010 criteria. MDPI 2020-07-07 /pmc/articles/PMC7400390/ /pubmed/32645820 http://dx.doi.org/10.3390/biomedicines8070196 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Alamo, Angela
De Luca, Claudia
Mongioì, Laura M.
Barbagallo, Federica
Cannarella, Rossella
La Vignera, Sandro
Calogero, Aldo E.
Condorelli, Rosita A.
Mitochondrial Membrane Potential Predicts 4-Hour Sperm Motility
title Mitochondrial Membrane Potential Predicts 4-Hour Sperm Motility
title_full Mitochondrial Membrane Potential Predicts 4-Hour Sperm Motility
title_fullStr Mitochondrial Membrane Potential Predicts 4-Hour Sperm Motility
title_full_unstemmed Mitochondrial Membrane Potential Predicts 4-Hour Sperm Motility
title_short Mitochondrial Membrane Potential Predicts 4-Hour Sperm Motility
title_sort mitochondrial membrane potential predicts 4-hour sperm motility
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400390/
https://www.ncbi.nlm.nih.gov/pubmed/32645820
http://dx.doi.org/10.3390/biomedicines8070196
work_keys_str_mv AT alamoangela mitochondrialmembranepotentialpredicts4hourspermmotility
AT delucaclaudia mitochondrialmembranepotentialpredicts4hourspermmotility
AT mongioilauram mitochondrialmembranepotentialpredicts4hourspermmotility
AT barbagallofederica mitochondrialmembranepotentialpredicts4hourspermmotility
AT cannarellarossella mitochondrialmembranepotentialpredicts4hourspermmotility
AT lavignerasandro mitochondrialmembranepotentialpredicts4hourspermmotility
AT calogeroaldoe mitochondrialmembranepotentialpredicts4hourspermmotility
AT condorellirositaa mitochondrialmembranepotentialpredicts4hourspermmotility