Cargando…

Profile of Enterobacteria Resistant to Beta-Lactams

A serious emerging problem worldwide is increased antimicrobial resistance. Acquisition of coding genes for evasion methods of antimicrobial drug mechanisms characterizes acquired resistance. This phenomenon has been observed in Enterobacteriaceae family. Treatment for bacterial infections is perfor...

Descripción completa

Detalles Bibliográficos
Autores principales: Santos, Andressa Liberal, dos Santos, Adailton Pereira, Ito, Célia Regina Malveste, de Queiroz, Pedro Henrique Pereira, de Almeida, Juliana Afonso, de Carvalho Júnior, Marcos Antonio Batista, de Oliveira, Camila Zanatta, Avelino, Melissa Ameloti G., Wastowski, Isabela Jubé, Gomes, Giselle Pinheiro Lima Aires, Souza, Adenícia Custódia Silva e, Vasconcelos, Lara Stefânia Netto de Oliveira Leão, Santos, Mônica de Oliveira, da Silva, Carla Afonso, Carneiro, Lilian Carla
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400480/
https://www.ncbi.nlm.nih.gov/pubmed/32679663
http://dx.doi.org/10.3390/antibiotics9070410
_version_ 1783566373437308928
author Santos, Andressa Liberal
dos Santos, Adailton Pereira
Ito, Célia Regina Malveste
de Queiroz, Pedro Henrique Pereira
de Almeida, Juliana Afonso
de Carvalho Júnior, Marcos Antonio Batista
de Oliveira, Camila Zanatta
Avelino, Melissa Ameloti G.
Wastowski, Isabela Jubé
Gomes, Giselle Pinheiro Lima Aires
Souza, Adenícia Custódia Silva e
Vasconcelos, Lara Stefânia Netto de Oliveira Leão
Santos, Mônica de Oliveira
da Silva, Carla Afonso
Carneiro, Lilian Carla
author_facet Santos, Andressa Liberal
dos Santos, Adailton Pereira
Ito, Célia Regina Malveste
de Queiroz, Pedro Henrique Pereira
de Almeida, Juliana Afonso
de Carvalho Júnior, Marcos Antonio Batista
de Oliveira, Camila Zanatta
Avelino, Melissa Ameloti G.
Wastowski, Isabela Jubé
Gomes, Giselle Pinheiro Lima Aires
Souza, Adenícia Custódia Silva e
Vasconcelos, Lara Stefânia Netto de Oliveira Leão
Santos, Mônica de Oliveira
da Silva, Carla Afonso
Carneiro, Lilian Carla
author_sort Santos, Andressa Liberal
collection PubMed
description A serious emerging problem worldwide is increased antimicrobial resistance. Acquisition of coding genes for evasion methods of antimicrobial drug mechanisms characterizes acquired resistance. This phenomenon has been observed in Enterobacteriaceae family. Treatment for bacterial infections is performed with antibiotics, of which the most used are beta-lactams. The aim of this study was to correlate antimicrobial resistance profiles in Enterobacteriaceae by phenotypic methods and molecular identification of 14 beta-lactamase coding genes. In this study, 70 exclusive isolates from Brazil were used, half of which were collected in veterinary clinics or hospitals Phenotypic methodologies were used and real-time PCR was the molecular methodology used, through the Sybr Green system. Regargding the results found in the tests it was observed that 74.28% were resistant to ampicillin, 62.85% were resistant to amoxicillin associated with clavalunate. The mechanism of resistance that presented the highest expression was ESBL (17.14%). The genes studied that were detected in a greater number of species were blaGIM and blaSIM (66.66% of the samples) and the one that was amplified in a smaller number of samples was blaVIM (16.66%). Therefore, high and worrying levels of antimicrobial resistance have been found in enterobacteria, and a way to minimize the accelerated emergence of their resistance includes developing or improving techniques that generate diagnoses with high efficiency and speed.
format Online
Article
Text
id pubmed-7400480
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74004802020-08-07 Profile of Enterobacteria Resistant to Beta-Lactams Santos, Andressa Liberal dos Santos, Adailton Pereira Ito, Célia Regina Malveste de Queiroz, Pedro Henrique Pereira de Almeida, Juliana Afonso de Carvalho Júnior, Marcos Antonio Batista de Oliveira, Camila Zanatta Avelino, Melissa Ameloti G. Wastowski, Isabela Jubé Gomes, Giselle Pinheiro Lima Aires Souza, Adenícia Custódia Silva e Vasconcelos, Lara Stefânia Netto de Oliveira Leão Santos, Mônica de Oliveira da Silva, Carla Afonso Carneiro, Lilian Carla Antibiotics (Basel) Article A serious emerging problem worldwide is increased antimicrobial resistance. Acquisition of coding genes for evasion methods of antimicrobial drug mechanisms characterizes acquired resistance. This phenomenon has been observed in Enterobacteriaceae family. Treatment for bacterial infections is performed with antibiotics, of which the most used are beta-lactams. The aim of this study was to correlate antimicrobial resistance profiles in Enterobacteriaceae by phenotypic methods and molecular identification of 14 beta-lactamase coding genes. In this study, 70 exclusive isolates from Brazil were used, half of which were collected in veterinary clinics or hospitals Phenotypic methodologies were used and real-time PCR was the molecular methodology used, through the Sybr Green system. Regargding the results found in the tests it was observed that 74.28% were resistant to ampicillin, 62.85% were resistant to amoxicillin associated with clavalunate. The mechanism of resistance that presented the highest expression was ESBL (17.14%). The genes studied that were detected in a greater number of species were blaGIM and blaSIM (66.66% of the samples) and the one that was amplified in a smaller number of samples was blaVIM (16.66%). Therefore, high and worrying levels of antimicrobial resistance have been found in enterobacteria, and a way to minimize the accelerated emergence of their resistance includes developing or improving techniques that generate diagnoses with high efficiency and speed. MDPI 2020-07-15 /pmc/articles/PMC7400480/ /pubmed/32679663 http://dx.doi.org/10.3390/antibiotics9070410 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Santos, Andressa Liberal
dos Santos, Adailton Pereira
Ito, Célia Regina Malveste
de Queiroz, Pedro Henrique Pereira
de Almeida, Juliana Afonso
de Carvalho Júnior, Marcos Antonio Batista
de Oliveira, Camila Zanatta
Avelino, Melissa Ameloti G.
Wastowski, Isabela Jubé
Gomes, Giselle Pinheiro Lima Aires
Souza, Adenícia Custódia Silva e
Vasconcelos, Lara Stefânia Netto de Oliveira Leão
Santos, Mônica de Oliveira
da Silva, Carla Afonso
Carneiro, Lilian Carla
Profile of Enterobacteria Resistant to Beta-Lactams
title Profile of Enterobacteria Resistant to Beta-Lactams
title_full Profile of Enterobacteria Resistant to Beta-Lactams
title_fullStr Profile of Enterobacteria Resistant to Beta-Lactams
title_full_unstemmed Profile of Enterobacteria Resistant to Beta-Lactams
title_short Profile of Enterobacteria Resistant to Beta-Lactams
title_sort profile of enterobacteria resistant to beta-lactams
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400480/
https://www.ncbi.nlm.nih.gov/pubmed/32679663
http://dx.doi.org/10.3390/antibiotics9070410
work_keys_str_mv AT santosandressaliberal profileofenterobacteriaresistanttobetalactams
AT dossantosadailtonpereira profileofenterobacteriaresistanttobetalactams
AT itoceliareginamalveste profileofenterobacteriaresistanttobetalactams
AT dequeirozpedrohenriquepereira profileofenterobacteriaresistanttobetalactams
AT dealmeidajulianaafonso profileofenterobacteriaresistanttobetalactams
AT decarvalhojuniormarcosantoniobatista profileofenterobacteriaresistanttobetalactams
AT deoliveiracamilazanatta profileofenterobacteriaresistanttobetalactams
AT avelinomelissaamelotig profileofenterobacteriaresistanttobetalactams
AT wastowskiisabelajube profileofenterobacteriaresistanttobetalactams
AT gomesgisellepinheirolimaaires profileofenterobacteriaresistanttobetalactams
AT souzaadeniciacustodiasilvae profileofenterobacteriaresistanttobetalactams
AT vasconceloslarastefanianettodeoliveiraleao profileofenterobacteriaresistanttobetalactams
AT santosmonicadeoliveira profileofenterobacteriaresistanttobetalactams
AT dasilvacarlaafonso profileofenterobacteriaresistanttobetalactams
AT carneiroliliancarla profileofenterobacteriaresistanttobetalactams