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Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy

The specific temporal evolution of bacterial and phage population sizes, in particular bacterial depletion and the emergence of a resistant bacterial population, can be seen as a kinetic fingerprint that depends on the manifold interactions of the specific phage–host pair during the course of infect...

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Autores principales: Loessner, Holger, Schlattmeier, Insea, Anders-Maurer, Marie, Bekeredjian-Ding, Isabelle, Rohde, Christine, Wittmann, Johannes, Pokalyuk, Cornelia, Krut, Oleg, Kamp, Christel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400656/
https://www.ncbi.nlm.nih.gov/pubmed/32674401
http://dx.doi.org/10.3390/antibiotics9070408
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author Loessner, Holger
Schlattmeier, Insea
Anders-Maurer, Marie
Bekeredjian-Ding, Isabelle
Rohde, Christine
Wittmann, Johannes
Pokalyuk, Cornelia
Krut, Oleg
Kamp, Christel
author_facet Loessner, Holger
Schlattmeier, Insea
Anders-Maurer, Marie
Bekeredjian-Ding, Isabelle
Rohde, Christine
Wittmann, Johannes
Pokalyuk, Cornelia
Krut, Oleg
Kamp, Christel
author_sort Loessner, Holger
collection PubMed
description The specific temporal evolution of bacterial and phage population sizes, in particular bacterial depletion and the emergence of a resistant bacterial population, can be seen as a kinetic fingerprint that depends on the manifold interactions of the specific phage–host pair during the course of infection. We have elaborated such a kinetic fingerprint for a human urinary tract Klebsiella pneumoniae isolate and its phage vB_KpnP_Lessing by a modeling approach based on data from in vitro co-culture. We found a faster depletion of the initially sensitive bacterial population than expected from simple mass action kinetics. A possible explanation for the rapid decline of the bacterial population is a synergistic interaction of phages which can be a favorable feature for phage therapies. In addition to this interaction characteristic, analysis of the kinetic fingerprint of this bacteria and phage combination revealed several relevant aspects of their population dynamics: A reduction of the bacterial concentration can be achieved only at high multiplicity of infection whereas bacterial extinction is hardly accomplished. Furthermore the binding affinity of the phage to bacteria is identified as one of the most crucial parameters for the reduction of the bacterial population size. Thus, kinetic fingerprinting can be used to infer phage–host interactions and to explore emergent dynamics which facilitates a rational design of phage therapies.
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spelling pubmed-74006562020-08-07 Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy Loessner, Holger Schlattmeier, Insea Anders-Maurer, Marie Bekeredjian-Ding, Isabelle Rohde, Christine Wittmann, Johannes Pokalyuk, Cornelia Krut, Oleg Kamp, Christel Antibiotics (Basel) Article The specific temporal evolution of bacterial and phage population sizes, in particular bacterial depletion and the emergence of a resistant bacterial population, can be seen as a kinetic fingerprint that depends on the manifold interactions of the specific phage–host pair during the course of infection. We have elaborated such a kinetic fingerprint for a human urinary tract Klebsiella pneumoniae isolate and its phage vB_KpnP_Lessing by a modeling approach based on data from in vitro co-culture. We found a faster depletion of the initially sensitive bacterial population than expected from simple mass action kinetics. A possible explanation for the rapid decline of the bacterial population is a synergistic interaction of phages which can be a favorable feature for phage therapies. In addition to this interaction characteristic, analysis of the kinetic fingerprint of this bacteria and phage combination revealed several relevant aspects of their population dynamics: A reduction of the bacterial concentration can be achieved only at high multiplicity of infection whereas bacterial extinction is hardly accomplished. Furthermore the binding affinity of the phage to bacteria is identified as one of the most crucial parameters for the reduction of the bacterial population size. Thus, kinetic fingerprinting can be used to infer phage–host interactions and to explore emergent dynamics which facilitates a rational design of phage therapies. MDPI 2020-07-14 /pmc/articles/PMC7400656/ /pubmed/32674401 http://dx.doi.org/10.3390/antibiotics9070408 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Loessner, Holger
Schlattmeier, Insea
Anders-Maurer, Marie
Bekeredjian-Ding, Isabelle
Rohde, Christine
Wittmann, Johannes
Pokalyuk, Cornelia
Krut, Oleg
Kamp, Christel
Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy
title Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy
title_full Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy
title_fullStr Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy
title_full_unstemmed Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy
title_short Kinetic Fingerprinting Links Bacteria-Phage Interactions with Emergent Dynamics: Rapid Depletion of Klebsiella pneumoniae Indicates Phage Synergy
title_sort kinetic fingerprinting links bacteria-phage interactions with emergent dynamics: rapid depletion of klebsiella pneumoniae indicates phage synergy
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400656/
https://www.ncbi.nlm.nih.gov/pubmed/32674401
http://dx.doi.org/10.3390/antibiotics9070408
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