Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples

Due to the current pandemic, a global shortage of reagents has drawn interest in developing alternatives to increase the number of coronavirus tests. One such alternative is sample pooling. We compared commercial kits that are used in COVID-19 diagnostics in terms of their sensitivity and feasibilit...

Descripción completa

Detalles Bibliográficos
Autores principales: Petrovan, Vlad, Vrajmasu, Virgil, Bucur, Ana Cristina, Soare, Dan Sebastian, Radu, Eugen, Dimon, Paula, Zaulet, Mihaela
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Article
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400658/
https://www.ncbi.nlm.nih.gov/pubmed/32664511
http://dx.doi.org/10.3390/diagnostics10070472
_version_ 1783566414661025792
author Petrovan, Vlad
Vrajmasu, Virgil
Bucur, Ana Cristina
Soare, Dan Sebastian
Radu, Eugen
Dimon, Paula
Zaulet, Mihaela
author_facet Petrovan, Vlad
Vrajmasu, Virgil
Bucur, Ana Cristina
Soare, Dan Sebastian
Radu, Eugen
Dimon, Paula
Zaulet, Mihaela
author_sort Petrovan, Vlad
collection PubMed
description Due to the current pandemic, a global shortage of reagents has drawn interest in developing alternatives to increase the number of coronavirus tests. One such alternative is sample pooling. We compared commercial kits that are used in COVID-19 diagnostics in terms of their sensitivity and feasibility for use in pooling. In this preliminary study, we showed that pooling of up to 80 samples did not affect the efficacy of the kits. Additionally, the RNA-dependent RNA polymerase (RdRp) gene is a more suitable target in pooled samples than the envelope (E) gene. This approach could provide an easy method of screening a large number of samples and help adjust different governmental regulations.
format Online
Article
Text
id pubmed-7400658
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74006582020-08-07 Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples Petrovan, Vlad Vrajmasu, Virgil Bucur, Ana Cristina Soare, Dan Sebastian Radu, Eugen Dimon, Paula Zaulet, Mihaela Diagnostics (Basel) Article Due to the current pandemic, a global shortage of reagents has drawn interest in developing alternatives to increase the number of coronavirus tests. One such alternative is sample pooling. We compared commercial kits that are used in COVID-19 diagnostics in terms of their sensitivity and feasibility for use in pooling. In this preliminary study, we showed that pooling of up to 80 samples did not affect the efficacy of the kits. Additionally, the RNA-dependent RNA polymerase (RdRp) gene is a more suitable target in pooled samples than the envelope (E) gene. This approach could provide an easy method of screening a large number of samples and help adjust different governmental regulations. MDPI 2020-07-11 /pmc/articles/PMC7400658/ /pubmed/32664511 http://dx.doi.org/10.3390/diagnostics10070472 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Petrovan, Vlad
Vrajmasu, Virgil
Bucur, Ana Cristina
Soare, Dan Sebastian
Radu, Eugen
Dimon, Paula
Zaulet, Mihaela
Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples
title Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples
title_full Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples
title_fullStr Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples
title_full_unstemmed Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples
title_short Evaluation of Commercial qPCR Kits for Detection of SARS-CoV-2 in Pooled Samples
title_sort evaluation of commercial qpcr kits for detection of sars-cov-2 in pooled samples
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400658/
https://www.ncbi.nlm.nih.gov/pubmed/32664511
http://dx.doi.org/10.3390/diagnostics10070472
work_keys_str_mv AT petrovanvlad evaluationofcommercialqpcrkitsfordetectionofsarscov2inpooledsamples
AT vrajmasuvirgil evaluationofcommercialqpcrkitsfordetectionofsarscov2inpooledsamples
AT bucuranacristina evaluationofcommercialqpcrkitsfordetectionofsarscov2inpooledsamples
AT soaredansebastian evaluationofcommercialqpcrkitsfordetectionofsarscov2inpooledsamples
AT radueugen evaluationofcommercialqpcrkitsfordetectionofsarscov2inpooledsamples
AT dimonpaula evaluationofcommercialqpcrkitsfordetectionofsarscov2inpooledsamples
AT zauletmihaela evaluationofcommercialqpcrkitsfordetectionofsarscov2inpooledsamples

Ejemplares similares