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Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma
Disorders of the oral mucosa are considered easy to diagnose since they can be visualized and examined directly. A change in the color of the oral mucosa reflects histopathological changes and is an important diagnostic parameter. However, the subjective perception of color varies. To determine the...
Autores principales: | , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
D.A. Spandidos
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400981/ https://www.ncbi.nlm.nih.gov/pubmed/32782532 http://dx.doi.org/10.3892/ol.2020.11772 |
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author | Sugahara, Keisuke Koyama, Yu Koyachi, Masahide Matsunaga, Satoru Odaka, Kento Kitamura, Kei Nakajima, Kei Matsuzaka, Kenichi Abe, Shinichi Katakura, Akira |
author_facet | Sugahara, Keisuke Koyama, Yu Koyachi, Masahide Matsunaga, Satoru Odaka, Kento Kitamura, Kei Nakajima, Kei Matsuzaka, Kenichi Abe, Shinichi Katakura, Akira |
author_sort | Sugahara, Keisuke |
collection | PubMed |
description | Disorders of the oral mucosa are considered easy to diagnose since they can be visualized and examined directly. A change in the color of the oral mucosa reflects histopathological changes and is an important diagnostic parameter. However, the subjective perception of color varies. To determine the extent of resection for oral mucosa conditions, it is necessary to digitize the color and perform objective assessments. In recent years, fluorescence visualization devices and analysis software that measure tissue luminance G have been employed for the identification of oral mucosa diseases. Fluorescence visualization is presumably based on the decrease in epithelial flavin adenine dinucleotide content and luminance G values due to the destruction of collagen cross-links [fluorescence visualization loss (FVL)]. However, cases with differences between luminance values and histopathological presentation exist. Therefore, additional factors may affect fluorescence visualization. The present study used a portable, non-contact oral mucosa fluorescence visualization device for luminance measurements in seven patients with oral squamous cell carcinoma. Furthermore, Picro-Sirius Red and immunohistochemical staining were performed for CK13, CK17, Ki67, p53 and E-cadherin in the FVL(+) (lesion) and FVL(−) (resection stump) areas to elucidate the principle of fluorescence visualization. Fluorescence was significantly lower in the FVL(+) than in the FVL(−) areas, and the mean luminance G value was 56. The Picro-Sirius Red stain revealed collagen destruction in the FVL(+) areas but no collagen disruption in the FVL(−) areas. CK13 was negative in the FVL(+) and positive in the FVL(−) areas, whereas the opposite pattern was observed for CK17. In the FVL(+) area, p53 staining was positive. E-cadherin expression was enhanced in the FVL(−) areas and reduced in the FVL(+) areas. Furthermore, the luminance G value tended to be lower in cases with weaker E-cadherin staining. The aforementioned results suggest that decreased E-cadherin expression may be a factor that regulates fluorescence visualization. |
format | Online Article Text |
id | pubmed-7400981 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | D.A. Spandidos |
record_format | MEDLINE/PubMed |
spelling | pubmed-74009812020-08-10 Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma Sugahara, Keisuke Koyama, Yu Koyachi, Masahide Matsunaga, Satoru Odaka, Kento Kitamura, Kei Nakajima, Kei Matsuzaka, Kenichi Abe, Shinichi Katakura, Akira Oncol Lett Articles Disorders of the oral mucosa are considered easy to diagnose since they can be visualized and examined directly. A change in the color of the oral mucosa reflects histopathological changes and is an important diagnostic parameter. However, the subjective perception of color varies. To determine the extent of resection for oral mucosa conditions, it is necessary to digitize the color and perform objective assessments. In recent years, fluorescence visualization devices and analysis software that measure tissue luminance G have been employed for the identification of oral mucosa diseases. Fluorescence visualization is presumably based on the decrease in epithelial flavin adenine dinucleotide content and luminance G values due to the destruction of collagen cross-links [fluorescence visualization loss (FVL)]. However, cases with differences between luminance values and histopathological presentation exist. Therefore, additional factors may affect fluorescence visualization. The present study used a portable, non-contact oral mucosa fluorescence visualization device for luminance measurements in seven patients with oral squamous cell carcinoma. Furthermore, Picro-Sirius Red and immunohistochemical staining were performed for CK13, CK17, Ki67, p53 and E-cadherin in the FVL(+) (lesion) and FVL(−) (resection stump) areas to elucidate the principle of fluorescence visualization. Fluorescence was significantly lower in the FVL(+) than in the FVL(−) areas, and the mean luminance G value was 56. The Picro-Sirius Red stain revealed collagen destruction in the FVL(+) areas but no collagen disruption in the FVL(−) areas. CK13 was negative in the FVL(+) and positive in the FVL(−) areas, whereas the opposite pattern was observed for CK17. In the FVL(+) area, p53 staining was positive. E-cadherin expression was enhanced in the FVL(−) areas and reduced in the FVL(+) areas. Furthermore, the luminance G value tended to be lower in cases with weaker E-cadherin staining. The aforementioned results suggest that decreased E-cadherin expression may be a factor that regulates fluorescence visualization. D.A. Spandidos 2020-09 2020-06-24 /pmc/articles/PMC7400981/ /pubmed/32782532 http://dx.doi.org/10.3892/ol.2020.11772 Text en Copyright: © Sugahara et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made. |
spellingShingle | Articles Sugahara, Keisuke Koyama, Yu Koyachi, Masahide Matsunaga, Satoru Odaka, Kento Kitamura, Kei Nakajima, Kei Matsuzaka, Kenichi Abe, Shinichi Katakura, Akira Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma |
title | Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma |
title_full | Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma |
title_fullStr | Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma |
title_full_unstemmed | Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma |
title_short | Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma |
title_sort | relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma |
topic | Articles |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400981/ https://www.ncbi.nlm.nih.gov/pubmed/32782532 http://dx.doi.org/10.3892/ol.2020.11772 |
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