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Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma

Disorders of the oral mucosa are considered easy to diagnose since they can be visualized and examined directly. A change in the color of the oral mucosa reflects histopathological changes and is an important diagnostic parameter. However, the subjective perception of color varies. To determine the...

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Autores principales: Sugahara, Keisuke, Koyama, Yu, Koyachi, Masahide, Matsunaga, Satoru, Odaka, Kento, Kitamura, Kei, Nakajima, Kei, Matsuzaka, Kenichi, Abe, Shinichi, Katakura, Akira
Formato: Online Artículo Texto
Lenguaje:English
Publicado: D.A. Spandidos 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400981/
https://www.ncbi.nlm.nih.gov/pubmed/32782532
http://dx.doi.org/10.3892/ol.2020.11772
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author Sugahara, Keisuke
Koyama, Yu
Koyachi, Masahide
Matsunaga, Satoru
Odaka, Kento
Kitamura, Kei
Nakajima, Kei
Matsuzaka, Kenichi
Abe, Shinichi
Katakura, Akira
author_facet Sugahara, Keisuke
Koyama, Yu
Koyachi, Masahide
Matsunaga, Satoru
Odaka, Kento
Kitamura, Kei
Nakajima, Kei
Matsuzaka, Kenichi
Abe, Shinichi
Katakura, Akira
author_sort Sugahara, Keisuke
collection PubMed
description Disorders of the oral mucosa are considered easy to diagnose since they can be visualized and examined directly. A change in the color of the oral mucosa reflects histopathological changes and is an important diagnostic parameter. However, the subjective perception of color varies. To determine the extent of resection for oral mucosa conditions, it is necessary to digitize the color and perform objective assessments. In recent years, fluorescence visualization devices and analysis software that measure tissue luminance G have been employed for the identification of oral mucosa diseases. Fluorescence visualization is presumably based on the decrease in epithelial flavin adenine dinucleotide content and luminance G values due to the destruction of collagen cross-links [fluorescence visualization loss (FVL)]. However, cases with differences between luminance values and histopathological presentation exist. Therefore, additional factors may affect fluorescence visualization. The present study used a portable, non-contact oral mucosa fluorescence visualization device for luminance measurements in seven patients with oral squamous cell carcinoma. Furthermore, Picro-Sirius Red and immunohistochemical staining were performed for CK13, CK17, Ki67, p53 and E-cadherin in the FVL(+) (lesion) and FVL(−) (resection stump) areas to elucidate the principle of fluorescence visualization. Fluorescence was significantly lower in the FVL(+) than in the FVL(−) areas, and the mean luminance G value was 56. The Picro-Sirius Red stain revealed collagen destruction in the FVL(+) areas but no collagen disruption in the FVL(−) areas. CK13 was negative in the FVL(+) and positive in the FVL(−) areas, whereas the opposite pattern was observed for CK17. In the FVL(+) area, p53 staining was positive. E-cadherin expression was enhanced in the FVL(−) areas and reduced in the FVL(+) areas. Furthermore, the luminance G value tended to be lower in cases with weaker E-cadherin staining. The aforementioned results suggest that decreased E-cadherin expression may be a factor that regulates fluorescence visualization.
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spelling pubmed-74009812020-08-10 Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma Sugahara, Keisuke Koyama, Yu Koyachi, Masahide Matsunaga, Satoru Odaka, Kento Kitamura, Kei Nakajima, Kei Matsuzaka, Kenichi Abe, Shinichi Katakura, Akira Oncol Lett Articles Disorders of the oral mucosa are considered easy to diagnose since they can be visualized and examined directly. A change in the color of the oral mucosa reflects histopathological changes and is an important diagnostic parameter. However, the subjective perception of color varies. To determine the extent of resection for oral mucosa conditions, it is necessary to digitize the color and perform objective assessments. In recent years, fluorescence visualization devices and analysis software that measure tissue luminance G have been employed for the identification of oral mucosa diseases. Fluorescence visualization is presumably based on the decrease in epithelial flavin adenine dinucleotide content and luminance G values due to the destruction of collagen cross-links [fluorescence visualization loss (FVL)]. However, cases with differences between luminance values and histopathological presentation exist. Therefore, additional factors may affect fluorescence visualization. The present study used a portable, non-contact oral mucosa fluorescence visualization device for luminance measurements in seven patients with oral squamous cell carcinoma. Furthermore, Picro-Sirius Red and immunohistochemical staining were performed for CK13, CK17, Ki67, p53 and E-cadherin in the FVL(+) (lesion) and FVL(−) (resection stump) areas to elucidate the principle of fluorescence visualization. Fluorescence was significantly lower in the FVL(+) than in the FVL(−) areas, and the mean luminance G value was 56. The Picro-Sirius Red stain revealed collagen destruction in the FVL(+) areas but no collagen disruption in the FVL(−) areas. CK13 was negative in the FVL(+) and positive in the FVL(−) areas, whereas the opposite pattern was observed for CK17. In the FVL(+) area, p53 staining was positive. E-cadherin expression was enhanced in the FVL(−) areas and reduced in the FVL(+) areas. Furthermore, the luminance G value tended to be lower in cases with weaker E-cadherin staining. The aforementioned results suggest that decreased E-cadherin expression may be a factor that regulates fluorescence visualization. D.A. Spandidos 2020-09 2020-06-24 /pmc/articles/PMC7400981/ /pubmed/32782532 http://dx.doi.org/10.3892/ol.2020.11772 Text en Copyright: © Sugahara et al. This is an open access article distributed under the terms of the Creative Commons Attribution-NonCommercial-NoDerivs License (https://creativecommons.org/licenses/by-nc-nd/4.0/) , which permits use and distribution in any medium, provided the original work is properly cited, the use is non-commercial and no modifications or adaptations are made.
spellingShingle Articles
Sugahara, Keisuke
Koyama, Yu
Koyachi, Masahide
Matsunaga, Satoru
Odaka, Kento
Kitamura, Kei
Nakajima, Kei
Matsuzaka, Kenichi
Abe, Shinichi
Katakura, Akira
Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma
title Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma
title_full Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma
title_fullStr Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma
title_full_unstemmed Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma
title_short Relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma
title_sort relationship between the immunohistological examination and fluorescence visualization of oral squamous cell carcinoma
topic Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7400981/
https://www.ncbi.nlm.nih.gov/pubmed/32782532
http://dx.doi.org/10.3892/ol.2020.11772
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