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In Vitro and In Vivo Antitumor Efficacy of Hizikia fusiforme Celluclast Extract against Bladder Cancer

Various physiological benefits have been linked to Hizikia fusiforme (HF), an edible brown seaweed. Here, fucose-containing sulfated polysaccharides were extracted from celluclast-processed HF (SPHF) and their antitumor efficacy against bladder cancer was evaluated in vitro and in vivo. SPHF possess...

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Autores principales: Song, Jun-Hui, Won, Se Yeon, Hwang, Byungdoo, Jung, Soontag, Choi, Changsun, Park, Sung-Soo, Choi, Yung Hyun, Kim, Wun-Jae, Moon, Sung-Kwon
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401265/
https://www.ncbi.nlm.nih.gov/pubmed/32708058
http://dx.doi.org/10.3390/nu12072159
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author Song, Jun-Hui
Won, Se Yeon
Hwang, Byungdoo
Jung, Soontag
Choi, Changsun
Park, Sung-Soo
Choi, Yung Hyun
Kim, Wun-Jae
Moon, Sung-Kwon
author_facet Song, Jun-Hui
Won, Se Yeon
Hwang, Byungdoo
Jung, Soontag
Choi, Changsun
Park, Sung-Soo
Choi, Yung Hyun
Kim, Wun-Jae
Moon, Sung-Kwon
author_sort Song, Jun-Hui
collection PubMed
description Various physiological benefits have been linked to Hizikia fusiforme (HF), an edible brown seaweed. Here, fucose-containing sulfated polysaccharides were extracted from celluclast-processed HF (SPHF) and their antitumor efficacy against bladder cancer was evaluated in vitro and in vivo. SPHF possesses high sulfated polysaccharide and fucose contents and free radical scavenging activities compared to those of celluclast-processed HF extracts (CHF). SPHF inhibited bladder cancer EJ cell proliferation via G1-phase cell cycle arrest. This was due to the induction of p21WAF1 expression associated with the downregulation of CDKs and cyclins. Moreover, JNK phosphorylation was identified as an SPHF-mediated signaling molecule. SPHF treatment also hindered the migration and invasion of EJ cells by inhibiting MMP-9 expression, which was attributed to the repression of transcriptional binding to NF-κB, AP-1, and Sp-1 in the MMP-9 promoter region. In an animal study, SPHF treatment suppressed EJ tumor growth in xenograft mice similarly to cisplatin. Furthermore, no toxicity signs were found after weight loss assessment, biochemical tests, and organ tissue immunostaining during oral administration of 20–200 mg/kg SPHF for 20 days. Therefore, our study demonstrates the antitumor efficacy of SPHF in vitro and in vivo, thus highlighting its potential for bladder cancer treatment development.
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spelling pubmed-74012652020-08-07 In Vitro and In Vivo Antitumor Efficacy of Hizikia fusiforme Celluclast Extract against Bladder Cancer Song, Jun-Hui Won, Se Yeon Hwang, Byungdoo Jung, Soontag Choi, Changsun Park, Sung-Soo Choi, Yung Hyun Kim, Wun-Jae Moon, Sung-Kwon Nutrients Article Various physiological benefits have been linked to Hizikia fusiforme (HF), an edible brown seaweed. Here, fucose-containing sulfated polysaccharides were extracted from celluclast-processed HF (SPHF) and their antitumor efficacy against bladder cancer was evaluated in vitro and in vivo. SPHF possesses high sulfated polysaccharide and fucose contents and free radical scavenging activities compared to those of celluclast-processed HF extracts (CHF). SPHF inhibited bladder cancer EJ cell proliferation via G1-phase cell cycle arrest. This was due to the induction of p21WAF1 expression associated with the downregulation of CDKs and cyclins. Moreover, JNK phosphorylation was identified as an SPHF-mediated signaling molecule. SPHF treatment also hindered the migration and invasion of EJ cells by inhibiting MMP-9 expression, which was attributed to the repression of transcriptional binding to NF-κB, AP-1, and Sp-1 in the MMP-9 promoter region. In an animal study, SPHF treatment suppressed EJ tumor growth in xenograft mice similarly to cisplatin. Furthermore, no toxicity signs were found after weight loss assessment, biochemical tests, and organ tissue immunostaining during oral administration of 20–200 mg/kg SPHF for 20 days. Therefore, our study demonstrates the antitumor efficacy of SPHF in vitro and in vivo, thus highlighting its potential for bladder cancer treatment development. MDPI 2020-07-21 /pmc/articles/PMC7401265/ /pubmed/32708058 http://dx.doi.org/10.3390/nu12072159 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Song, Jun-Hui
Won, Se Yeon
Hwang, Byungdoo
Jung, Soontag
Choi, Changsun
Park, Sung-Soo
Choi, Yung Hyun
Kim, Wun-Jae
Moon, Sung-Kwon
In Vitro and In Vivo Antitumor Efficacy of Hizikia fusiforme Celluclast Extract against Bladder Cancer
title In Vitro and In Vivo Antitumor Efficacy of Hizikia fusiforme Celluclast Extract against Bladder Cancer
title_full In Vitro and In Vivo Antitumor Efficacy of Hizikia fusiforme Celluclast Extract against Bladder Cancer
title_fullStr In Vitro and In Vivo Antitumor Efficacy of Hizikia fusiforme Celluclast Extract against Bladder Cancer
title_full_unstemmed In Vitro and In Vivo Antitumor Efficacy of Hizikia fusiforme Celluclast Extract against Bladder Cancer
title_short In Vitro and In Vivo Antitumor Efficacy of Hizikia fusiforme Celluclast Extract against Bladder Cancer
title_sort in vitro and in vivo antitumor efficacy of hizikia fusiforme celluclast extract against bladder cancer
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401265/
https://www.ncbi.nlm.nih.gov/pubmed/32708058
http://dx.doi.org/10.3390/nu12072159
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