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Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion

The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This...

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Autores principales: Pacheco, Luz Verónica, Parada, Javier, Pérez-Correa, José Ricardo, Mariotti-Celis, María Salomé, Erpel, Fernanda, Zambrano, Angara, Palacios, Mauricio
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401274/
https://www.ncbi.nlm.nih.gov/pubmed/32650394
http://dx.doi.org/10.3390/md18070353
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author Pacheco, Luz Verónica
Parada, Javier
Pérez-Correa, José Ricardo
Mariotti-Celis, María Salomé
Erpel, Fernanda
Zambrano, Angara
Palacios, Mauricio
author_facet Pacheco, Luz Verónica
Parada, Javier
Pérez-Correa, José Ricardo
Mariotti-Celis, María Salomé
Erpel, Fernanda
Zambrano, Angara
Palacios, Mauricio
author_sort Pacheco, Luz Verónica
collection PubMed
description The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This research explored the potential of six common Chilean seaweeds to obtain anti-hyperglycemic polyphenol extracts, based on their capacity to inhibit key enzymes related with starch digestion. Ethanol/water hot pressurized liquid extraction (HPLE), which is an environmentally friendly method, was studied and compared to conventional extraction with acetone. Total polyphenols (TP), antioxidant activity, cytotoxicity and inhibition capacity on α-glucosidase and α-amylase were analyzed. Results showed that the Durvillaea antarctica (cochayuyo) acetone extract had the highest TP content (6.7 ± 0.7 mg gallic acid equivalents (GAE)/g dry seaweed), while its HPLE ethanol/water extract showed the highest antioxidant activity (680.1 ± 11.6 μmol E Trolox/g dry seaweed). No extract affected cell viability significantly. Only cochayuyo produced extracts having relevant anti-enzymatic capacity on both studied enzymes, showing a much stronger inhibition to α-glucosidase (even almost 100% at 1000 µg/mL) than to α-amylase. In conclusion, from the Chilean seaweeds considered in this study, cochayuyo is the most suitable for developing functional ingredients to moderate postprandial glycemic response (starchy foods), since it showed a clear enzymatic inhibition capacity and selectivity.
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spelling pubmed-74012742020-08-11 Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion Pacheco, Luz Verónica Parada, Javier Pérez-Correa, José Ricardo Mariotti-Celis, María Salomé Erpel, Fernanda Zambrano, Angara Palacios, Mauricio Mar Drugs Article The increment of non-communicable chronic diseases is a constant concern worldwide, with type-2 diabetes mellitus being one of the most common illnesses. A mechanism to avoid diabetes-related hyperglycemia is to reduce food digestion/absorption by using anti-enzymatic (functional) ingredients. This research explored the potential of six common Chilean seaweeds to obtain anti-hyperglycemic polyphenol extracts, based on their capacity to inhibit key enzymes related with starch digestion. Ethanol/water hot pressurized liquid extraction (HPLE), which is an environmentally friendly method, was studied and compared to conventional extraction with acetone. Total polyphenols (TP), antioxidant activity, cytotoxicity and inhibition capacity on α-glucosidase and α-amylase were analyzed. Results showed that the Durvillaea antarctica (cochayuyo) acetone extract had the highest TP content (6.7 ± 0.7 mg gallic acid equivalents (GAE)/g dry seaweed), while its HPLE ethanol/water extract showed the highest antioxidant activity (680.1 ± 11.6 μmol E Trolox/g dry seaweed). No extract affected cell viability significantly. Only cochayuyo produced extracts having relevant anti-enzymatic capacity on both studied enzymes, showing a much stronger inhibition to α-glucosidase (even almost 100% at 1000 µg/mL) than to α-amylase. In conclusion, from the Chilean seaweeds considered in this study, cochayuyo is the most suitable for developing functional ingredients to moderate postprandial glycemic response (starchy foods), since it showed a clear enzymatic inhibition capacity and selectivity. MDPI 2020-07-08 /pmc/articles/PMC7401274/ /pubmed/32650394 http://dx.doi.org/10.3390/md18070353 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Pacheco, Luz Verónica
Parada, Javier
Pérez-Correa, José Ricardo
Mariotti-Celis, María Salomé
Erpel, Fernanda
Zambrano, Angara
Palacios, Mauricio
Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion
title Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion
title_full Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion
title_fullStr Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion
title_full_unstemmed Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion
title_short Bioactive Polyphenols from Southern Chile Seaweed as Inhibitors of Enzymes for Starch Digestion
title_sort bioactive polyphenols from southern chile seaweed as inhibitors of enzymes for starch digestion
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401274/
https://www.ncbi.nlm.nih.gov/pubmed/32650394
http://dx.doi.org/10.3390/md18070353
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