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Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis
SIMPLE SUMMARY: Inflammatory bowel disease (IBD) is extremely harmful to animal health and can affect animal growth and production. Dextran sulfate sodium (DSS) can cause IBD in animals, resulting in diarrhea and bloody stools. The present study aimed to determine whether oral sodium butyrate can re...
Autores principales: | , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401615/ https://www.ncbi.nlm.nih.gov/pubmed/32645998 http://dx.doi.org/10.3390/ani10071154 |
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author | Dou, Xiujing Gao, Nan Yan, Di Shan, Anshan |
author_facet | Dou, Xiujing Gao, Nan Yan, Di Shan, Anshan |
author_sort | Dou, Xiujing |
collection | PubMed |
description | SIMPLE SUMMARY: Inflammatory bowel disease (IBD) is extremely harmful to animal health and can affect animal growth and production. Dextran sulfate sodium (DSS) can cause IBD in animals, resulting in diarrhea and bloody stools. The present study aimed to determine whether oral sodium butyrate can relieve DSS-induced colitis in mice. By using histological evaluation (H&E) staining technology and 16S rRNA sequence analysis, we found that the severity of colitis in mice receiving oral sodium butyrate was reduced, and the composition of gut microbiota was changed. These results indicate that sodium butyrate can relieve DSS-induced colitis in mice by restoring the balance of gut microbiota dysbiosis. ABSTRACT: Inflammatory bowel disease (IBD) develops as a result of complicated interactions between genetic susceptibility, excessive innate immunity, and environmental factors, which are mainly related to the gut microbiota. The present study aimed to elucidate the protective effects and underlying mechanisms of a short-chain fatty acid salt, sodium butyrate, on colonic inflammation induced by dextran sulfate sodium (DSS) in mice. Pretreatment with sodium butyrate attenuated colitis, as demonstrated by the decreased disease activity index (DAI), colon length shortening, spleen tumidness, and histopathology scores, while maintaining intestinal barrier integrity, as observed by H&E staining and electron microscopy. 16S rRNA sequence analysis revealed that sodium butyrate caused a remarkable alteration of the gut microbiota. Bacteroides, Lachnospiraceae, the Lachnospiraceae NK4A136 group, and Ruminiclostridium 6 presented dramatic differences after sodium butyrate supplementation. This work verifies that sodium butyrate can improve mouse colitis via microbe–host interactions by regulating the microbial community. Taken together, the findings demonstrate that sodium butyrate shows great potential as a probiotic agent for ameliorating colitis. |
format | Online Article Text |
id | pubmed-7401615 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74016152020-08-07 Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis Dou, Xiujing Gao, Nan Yan, Di Shan, Anshan Animals (Basel) Article SIMPLE SUMMARY: Inflammatory bowel disease (IBD) is extremely harmful to animal health and can affect animal growth and production. Dextran sulfate sodium (DSS) can cause IBD in animals, resulting in diarrhea and bloody stools. The present study aimed to determine whether oral sodium butyrate can relieve DSS-induced colitis in mice. By using histological evaluation (H&E) staining technology and 16S rRNA sequence analysis, we found that the severity of colitis in mice receiving oral sodium butyrate was reduced, and the composition of gut microbiota was changed. These results indicate that sodium butyrate can relieve DSS-induced colitis in mice by restoring the balance of gut microbiota dysbiosis. ABSTRACT: Inflammatory bowel disease (IBD) develops as a result of complicated interactions between genetic susceptibility, excessive innate immunity, and environmental factors, which are mainly related to the gut microbiota. The present study aimed to elucidate the protective effects and underlying mechanisms of a short-chain fatty acid salt, sodium butyrate, on colonic inflammation induced by dextran sulfate sodium (DSS) in mice. Pretreatment with sodium butyrate attenuated colitis, as demonstrated by the decreased disease activity index (DAI), colon length shortening, spleen tumidness, and histopathology scores, while maintaining intestinal barrier integrity, as observed by H&E staining and electron microscopy. 16S rRNA sequence analysis revealed that sodium butyrate caused a remarkable alteration of the gut microbiota. Bacteroides, Lachnospiraceae, the Lachnospiraceae NK4A136 group, and Ruminiclostridium 6 presented dramatic differences after sodium butyrate supplementation. This work verifies that sodium butyrate can improve mouse colitis via microbe–host interactions by regulating the microbial community. Taken together, the findings demonstrate that sodium butyrate shows great potential as a probiotic agent for ameliorating colitis. MDPI 2020-07-07 /pmc/articles/PMC7401615/ /pubmed/32645998 http://dx.doi.org/10.3390/ani10071154 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Dou, Xiujing Gao, Nan Yan, Di Shan, Anshan Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis |
title | Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis |
title_full | Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis |
title_fullStr | Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis |
title_full_unstemmed | Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis |
title_short | Sodium Butyrate Alleviates Mouse Colitis by Regulating Gut Microbiota Dysbiosis |
title_sort | sodium butyrate alleviates mouse colitis by regulating gut microbiota dysbiosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401615/ https://www.ncbi.nlm.nih.gov/pubmed/32645998 http://dx.doi.org/10.3390/ani10071154 |
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