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ERdj8 governs the size of autophagosomes during the formation process

In macroautophagy, membrane structures called autophagosomes engulf substrates and deliver them for lysosomal degradation. Autophagosomes enwrap a variety of targets with diverse sizes, from portions of cytosol to larger organelles. However, the mechanism by which autophagosome size is controlled re...

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Autores principales: Yamamoto, Yo-hei, Kasai, Ayano, Omori, Hiroko, Takino, Tomoe, Sugihara, Munechika, Umemoto, Tetsuo, Hamasaki, Maho, Hatta, Tomohisa, Natsume, Tohru, Morimoto, Richard I., Arai, Ritsuko, Waguri, Satoshi, Sato, Miyuki, Sato, Ken, Bar-Nun, Shoshana, Yoshimori, Tamotsu, Noda, Takeshi, Nagata, Kazuhiro
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Rockefeller University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401821/
https://www.ncbi.nlm.nih.gov/pubmed/32492081
http://dx.doi.org/10.1083/jcb.201903127
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author Yamamoto, Yo-hei
Kasai, Ayano
Omori, Hiroko
Takino, Tomoe
Sugihara, Munechika
Umemoto, Tetsuo
Hamasaki, Maho
Hatta, Tomohisa
Natsume, Tohru
Morimoto, Richard I.
Arai, Ritsuko
Waguri, Satoshi
Sato, Miyuki
Sato, Ken
Bar-Nun, Shoshana
Yoshimori, Tamotsu
Noda, Takeshi
Nagata, Kazuhiro
author_facet Yamamoto, Yo-hei
Kasai, Ayano
Omori, Hiroko
Takino, Tomoe
Sugihara, Munechika
Umemoto, Tetsuo
Hamasaki, Maho
Hatta, Tomohisa
Natsume, Tohru
Morimoto, Richard I.
Arai, Ritsuko
Waguri, Satoshi
Sato, Miyuki
Sato, Ken
Bar-Nun, Shoshana
Yoshimori, Tamotsu
Noda, Takeshi
Nagata, Kazuhiro
author_sort Yamamoto, Yo-hei
collection PubMed
description In macroautophagy, membrane structures called autophagosomes engulf substrates and deliver them for lysosomal degradation. Autophagosomes enwrap a variety of targets with diverse sizes, from portions of cytosol to larger organelles. However, the mechanism by which autophagosome size is controlled remains elusive. We characterized a novel ER membrane protein, ERdj8, in mammalian cells. ERdj8 localizes to a meshwork-like ER subdomain along with phosphatidylinositol synthase (PIS) and autophagy-related (Atg) proteins. ERdj8 overexpression extended the size of the autophagosome through its DnaJ and TRX domains. ERdj8 ablation resulted in a defect in engulfing larger targets. C. elegans, in which the ERdj8 orthologue dnj-8 was knocked down, could perform autophagy on smaller mitochondria derived from the paternal lineage but not the somatic mitochondria. Thus, ERdj8 may play a critical role in autophagosome formation by providing the capacity to target substrates of diverse sizes for degradation.
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spelling pubmed-74018212021-02-03 ERdj8 governs the size of autophagosomes during the formation process Yamamoto, Yo-hei Kasai, Ayano Omori, Hiroko Takino, Tomoe Sugihara, Munechika Umemoto, Tetsuo Hamasaki, Maho Hatta, Tomohisa Natsume, Tohru Morimoto, Richard I. Arai, Ritsuko Waguri, Satoshi Sato, Miyuki Sato, Ken Bar-Nun, Shoshana Yoshimori, Tamotsu Noda, Takeshi Nagata, Kazuhiro J Cell Biol Article In macroautophagy, membrane structures called autophagosomes engulf substrates and deliver them for lysosomal degradation. Autophagosomes enwrap a variety of targets with diverse sizes, from portions of cytosol to larger organelles. However, the mechanism by which autophagosome size is controlled remains elusive. We characterized a novel ER membrane protein, ERdj8, in mammalian cells. ERdj8 localizes to a meshwork-like ER subdomain along with phosphatidylinositol synthase (PIS) and autophagy-related (Atg) proteins. ERdj8 overexpression extended the size of the autophagosome through its DnaJ and TRX domains. ERdj8 ablation resulted in a defect in engulfing larger targets. C. elegans, in which the ERdj8 orthologue dnj-8 was knocked down, could perform autophagy on smaller mitochondria derived from the paternal lineage but not the somatic mitochondria. Thus, ERdj8 may play a critical role in autophagosome formation by providing the capacity to target substrates of diverse sizes for degradation. Rockefeller University Press 2020-06-03 /pmc/articles/PMC7401821/ /pubmed/32492081 http://dx.doi.org/10.1083/jcb.201903127 Text en © 2020 Yamamoto et al. http://www.rupress.org/terms/https://creativecommons.org/licenses/by-nc-sa/4.0/This article is distributed under the terms of an Attribution–Noncommercial–Share Alike–No Mirror Sites license for the first six months after the publication date (see http://www.rupress.org/terms/). After six months it is available under a Creative Commons License (Attribution–Noncommercial–Share Alike 4.0 International license, as described at https://creativecommons.org/licenses/by-nc-sa/4.0/).
spellingShingle Article
Yamamoto, Yo-hei
Kasai, Ayano
Omori, Hiroko
Takino, Tomoe
Sugihara, Munechika
Umemoto, Tetsuo
Hamasaki, Maho
Hatta, Tomohisa
Natsume, Tohru
Morimoto, Richard I.
Arai, Ritsuko
Waguri, Satoshi
Sato, Miyuki
Sato, Ken
Bar-Nun, Shoshana
Yoshimori, Tamotsu
Noda, Takeshi
Nagata, Kazuhiro
ERdj8 governs the size of autophagosomes during the formation process
title ERdj8 governs the size of autophagosomes during the formation process
title_full ERdj8 governs the size of autophagosomes during the formation process
title_fullStr ERdj8 governs the size of autophagosomes during the formation process
title_full_unstemmed ERdj8 governs the size of autophagosomes during the formation process
title_short ERdj8 governs the size of autophagosomes during the formation process
title_sort erdj8 governs the size of autophagosomes during the formation process
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7401821/
https://www.ncbi.nlm.nih.gov/pubmed/32492081
http://dx.doi.org/10.1083/jcb.201903127
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