A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus

A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (ant...

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Autores principales: Wei, Yanqiu, Yang, Baozhi, Li, Yunlong, Duan, Yongcheng, Tian, Deyu, He, Baoxiang, Chen, Chuangfu, Liu, Wenjun, Yang, Limin
Formato: Online Artículo Texto
Lenguaje:English
Publicado: The Korean Society of Veterinary Science 2020
Materias:
Rapid Communication
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7402939/
https://www.ncbi.nlm.nih.gov/pubmed/32735103
http://dx.doi.org/10.4142/jvs.2020.21.e68
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author Wei, Yanqiu
Yang, Baozhi
Li, Yunlong
Duan, Yongcheng
Tian, Deyu
He, Baoxiang
Chen, Chuangfu
Liu, Wenjun
Yang, Limin
author_facet Wei, Yanqiu
Yang, Baozhi
Li, Yunlong
Duan, Yongcheng
Tian, Deyu
He, Baoxiang
Chen, Chuangfu
Liu, Wenjun
Yang, Limin
author_sort Wei, Yanqiu
collection PubMed
description A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min.
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spelling pubmed-74029392020-08-11 A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus Wei, Yanqiu Yang, Baozhi Li, Yunlong Duan, Yongcheng Tian, Deyu He, Baoxiang Chen, Chuangfu Liu, Wenjun Yang, Limin J Vet Sci Rapid Communication A fluorescent microsphere-based immunochromatographic strip test (FICT) was developed for the rapid, sensitive, and quantitative detection of porcine reproductive and respiratory syndrome virus (PRRSV) antibodies at the pen-side. The assay was based on the formation of a sandwich immune-complex (anti-pig IgG-PRRSV antibodies-NSP7/N), which was validated by a comparison with IDEXX-ELISA using 3325 clinical specimens. The diagnostic specificity, sensitivity, and accuracy of FICT were 97.28, 93.41, and 94.95%, respectively. FICT showed a good correlation with the virus neutralization assay. Overall, a promising pen-side diagnostic tool was developed for the rapid and quantitative detection of PRRSV antibodies within 15 min. The Korean Society of Veterinary Science 2020-07 2020-07-15 /pmc/articles/PMC7402939/ /pubmed/32735103 http://dx.doi.org/10.4142/jvs.2020.21.e68 Text en © 2020 The Korean Society of Veterinary Science https://creativecommons.org/licenses/by-nc/4.0 This is an Open Access article distributed under the terms of the Creative Commons Attribution Non-Commercial License (https://creativecommons.org/licenses/by-nc/4.0) which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Rapid Communication
Wei, Yanqiu
Yang, Baozhi
Li, Yunlong
Duan, Yongcheng
Tian, Deyu
He, Baoxiang
Chen, Chuangfu
Liu, Wenjun
Yang, Limin
A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus
title A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus
title_full A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus
title_fullStr A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus
title_full_unstemmed A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus
title_short A rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus
title_sort rapid and quantitative fluorescent microsphere immunochromatographic strip test for detection of antibodies to porcine reproductive and respiratory syndrome virus
topic Rapid Communication
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7402939/
https://www.ncbi.nlm.nih.gov/pubmed/32735103
http://dx.doi.org/10.4142/jvs.2020.21.e68
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