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Rapid Accumulation of Mutations in Growing Mycelia of a Hypervariable Fungus Schizophyllum commune

The basidiomycete Schizophyllum commune has the highest level of genetic polymorphism known among living organisms. In a previous study, it was also found to have a moderately high per-generation mutation rate of 2×10(−8), likely contributing to its high polymorphism. However, this rate has been mea...

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Autores principales: Bezmenova, Aleksandra V, Zvyagina, Elena A, Fedotova, Anna V, Kasianov, Artem S, Neretina, Tatiana V, Penin, Aleksey A, Bazykin, Georgii A, Kondrashov, Alexey S
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Oxford University Press 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403608/
https://www.ncbi.nlm.nih.gov/pubmed/32243532
http://dx.doi.org/10.1093/molbev/msaa083
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author Bezmenova, Aleksandra V
Zvyagina, Elena A
Fedotova, Anna V
Kasianov, Artem S
Neretina, Tatiana V
Penin, Aleksey A
Bazykin, Georgii A
Kondrashov, Alexey S
author_facet Bezmenova, Aleksandra V
Zvyagina, Elena A
Fedotova, Anna V
Kasianov, Artem S
Neretina, Tatiana V
Penin, Aleksey A
Bazykin, Georgii A
Kondrashov, Alexey S
author_sort Bezmenova, Aleksandra V
collection PubMed
description The basidiomycete Schizophyllum commune has the highest level of genetic polymorphism known among living organisms. In a previous study, it was also found to have a moderately high per-generation mutation rate of 2×10(−8), likely contributing to its high polymorphism. However, this rate has been measured only in an experiment on Petri dishes, and it is unclear how it translates to natural populations. Here, we used an experimental design that measures the rate of accumulation of de novo mutations in a linearly growing mycelium. We show that S. commune accumulates mutations at a rate of 1.24×10(−7) substitutions per nucleotide per meter of growth, or ∼2.04×10(−11) per nucleotide per cell division. In contrast to what has been observed in a number of species with extensive vegetative growth, this rate does not decline in the course of propagation of a mycelium. As a result, even a moderate per-cell-division mutation rate in S. commune can translate into a very high per-generation mutation rate when the number of cell divisions between consecutive meiosis is large.
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spelling pubmed-74036082020-08-07 Rapid Accumulation of Mutations in Growing Mycelia of a Hypervariable Fungus Schizophyllum commune Bezmenova, Aleksandra V Zvyagina, Elena A Fedotova, Anna V Kasianov, Artem S Neretina, Tatiana V Penin, Aleksey A Bazykin, Georgii A Kondrashov, Alexey S Mol Biol Evol Discoveries The basidiomycete Schizophyllum commune has the highest level of genetic polymorphism known among living organisms. In a previous study, it was also found to have a moderately high per-generation mutation rate of 2×10(−8), likely contributing to its high polymorphism. However, this rate has been measured only in an experiment on Petri dishes, and it is unclear how it translates to natural populations. Here, we used an experimental design that measures the rate of accumulation of de novo mutations in a linearly growing mycelium. We show that S. commune accumulates mutations at a rate of 1.24×10(−7) substitutions per nucleotide per meter of growth, or ∼2.04×10(−11) per nucleotide per cell division. In contrast to what has been observed in a number of species with extensive vegetative growth, this rate does not decline in the course of propagation of a mycelium. As a result, even a moderate per-cell-division mutation rate in S. commune can translate into a very high per-generation mutation rate when the number of cell divisions between consecutive meiosis is large. Oxford University Press 2020-08 2020-04-06 /pmc/articles/PMC7403608/ /pubmed/32243532 http://dx.doi.org/10.1093/molbev/msaa083 Text en © The Author(s) 2020. Published by Oxford University Press on behalf of the Society for Molecular Biology and Evolution. http://creativecommons.org/licenses/by/4.0/ This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
spellingShingle Discoveries
Bezmenova, Aleksandra V
Zvyagina, Elena A
Fedotova, Anna V
Kasianov, Artem S
Neretina, Tatiana V
Penin, Aleksey A
Bazykin, Georgii A
Kondrashov, Alexey S
Rapid Accumulation of Mutations in Growing Mycelia of a Hypervariable Fungus Schizophyllum commune
title Rapid Accumulation of Mutations in Growing Mycelia of a Hypervariable Fungus Schizophyllum commune
title_full Rapid Accumulation of Mutations in Growing Mycelia of a Hypervariable Fungus Schizophyllum commune
title_fullStr Rapid Accumulation of Mutations in Growing Mycelia of a Hypervariable Fungus Schizophyllum commune
title_full_unstemmed Rapid Accumulation of Mutations in Growing Mycelia of a Hypervariable Fungus Schizophyllum commune
title_short Rapid Accumulation of Mutations in Growing Mycelia of a Hypervariable Fungus Schizophyllum commune
title_sort rapid accumulation of mutations in growing mycelia of a hypervariable fungus schizophyllum commune
topic Discoveries
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403608/
https://www.ncbi.nlm.nih.gov/pubmed/32243532
http://dx.doi.org/10.1093/molbev/msaa083
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