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Assessment of Expression of Homeobox A5 in Endometrial Cancer on the mRNA and Protein Level

BACKGROUND: Endometrial cancer is one of the most common gynecological cancer in the developed countries and occurs mainly in postmenopausal women. Angiogenesis is important for cancer formation as it provides nutrients for growing tumor mass. Most tumors do not show detectable Homeobox A5 (HOXA5 le...

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Autores principales: Dziobek, Konrad, Oplawski, Marcin, Zmarzły, Nikola, Gabarek, Beniamin O., Kiełbasiński, Robert, Kiełbasiński, Kamil, Kieszkowski, Przemysław, Talkowski, Karol, Boroń, Dariusz
Formato: Online Artículo Texto
Lenguaje:English
Publicado: Bentham Science Publishers 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403687/
https://www.ncbi.nlm.nih.gov/pubmed/31880256
http://dx.doi.org/10.2174/1389201021666191227121627
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author Dziobek, Konrad
Oplawski, Marcin
Zmarzły, Nikola
Gabarek, Beniamin O.
Kiełbasiński, Robert
Kiełbasiński, Kamil
Kieszkowski, Przemysław
Talkowski, Karol
Boroń, Dariusz
author_facet Dziobek, Konrad
Oplawski, Marcin
Zmarzły, Nikola
Gabarek, Beniamin O.
Kiełbasiński, Robert
Kiełbasiński, Kamil
Kieszkowski, Przemysław
Talkowski, Karol
Boroń, Dariusz
author_sort Dziobek, Konrad
collection PubMed
description BACKGROUND: Endometrial cancer is one of the most common gynecological cancer in the developed countries and occurs mainly in postmenopausal women. Angiogenesis is important for cancer formation as it provides nutrients for growing tumor mass. Most tumors do not show detectable Homeobox A5 (HOXA5 level), suggesting its potential role as a cancer suppressor. It was demonstrated that HOXA5 is involved in the progression of various types of cancer and the loss of its expression correlates with higher pathological grade and poorer outcome. OBJECTIVE: The aim of the study was to evaluate HOXA5 expression at transcriptome and protein levels. MATERIALS AND METHODS: The study enrolled 45 women diagnosed with endometrial cancer and 15 without neoplastic changes. The histopathological examination allowed us to divide cancer tissue samples according to the degree of histological differentiation: G1, 17; G2, 15; G3, 13. The expression of the HOXA5 protein was determined by immunohistochemistry. Microarray and RT-qPCR techniques were used to assess HOXA5 expression at the mRNA level. RESULTS: The reaction to the HOXA5 protein was only visible in glandular cells in G1 endometrial cancer and was lower compared to the control. In grades 2 and 3, reactions were noted at the limit of the method’s sensitivity. In addition, reduced HOXA5 expression was observed at the transcriptome level. CONCLUSION: HOXA5 may become a potential complementary molecular marker, allowing early detection of neoplastic changes in the endometrium. It also seems that detection of HOXA5 at the mRNA and protein levels may be helpful in improving the accuracy of diagnosis and planning effective oncological therapy.
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spelling pubmed-74036872020-08-17 Assessment of Expression of Homeobox A5 in Endometrial Cancer on the mRNA and Protein Level Dziobek, Konrad Oplawski, Marcin Zmarzły, Nikola Gabarek, Beniamin O. Kiełbasiński, Robert Kiełbasiński, Kamil Kieszkowski, Przemysław Talkowski, Karol Boroń, Dariusz Curr Pharm Biotechnol Article BACKGROUND: Endometrial cancer is one of the most common gynecological cancer in the developed countries and occurs mainly in postmenopausal women. Angiogenesis is important for cancer formation as it provides nutrients for growing tumor mass. Most tumors do not show detectable Homeobox A5 (HOXA5 level), suggesting its potential role as a cancer suppressor. It was demonstrated that HOXA5 is involved in the progression of various types of cancer and the loss of its expression correlates with higher pathological grade and poorer outcome. OBJECTIVE: The aim of the study was to evaluate HOXA5 expression at transcriptome and protein levels. MATERIALS AND METHODS: The study enrolled 45 women diagnosed with endometrial cancer and 15 without neoplastic changes. The histopathological examination allowed us to divide cancer tissue samples according to the degree of histological differentiation: G1, 17; G2, 15; G3, 13. The expression of the HOXA5 protein was determined by immunohistochemistry. Microarray and RT-qPCR techniques were used to assess HOXA5 expression at the mRNA level. RESULTS: The reaction to the HOXA5 protein was only visible in glandular cells in G1 endometrial cancer and was lower compared to the control. In grades 2 and 3, reactions were noted at the limit of the method’s sensitivity. In addition, reduced HOXA5 expression was observed at the transcriptome level. CONCLUSION: HOXA5 may become a potential complementary molecular marker, allowing early detection of neoplastic changes in the endometrium. It also seems that detection of HOXA5 at the mRNA and protein levels may be helpful in improving the accuracy of diagnosis and planning effective oncological therapy. Bentham Science Publishers 2020-06 2020-06 /pmc/articles/PMC7403687/ /pubmed/31880256 http://dx.doi.org/10.2174/1389201021666191227121627 Text en © 2020 Bentham Science Publishers https://creativecommons.org/licenses/by-nc/4.0/legalcode This is an open access article licensed under the terms of the Creative Commons Attribution-Non-Commercial 4.0 International Public License (CC BY-NC 4.0) (https://creativecommons.org/licenses/by-nc/4.0/legalcode), which permits unrestricted, non-commercial use, distribution and reproduction in any medium, provided the work is properly cited.
spellingShingle Article
Dziobek, Konrad
Oplawski, Marcin
Zmarzły, Nikola
Gabarek, Beniamin O.
Kiełbasiński, Robert
Kiełbasiński, Kamil
Kieszkowski, Przemysław
Talkowski, Karol
Boroń, Dariusz
Assessment of Expression of Homeobox A5 in Endometrial Cancer on the mRNA and Protein Level
title Assessment of Expression of Homeobox A5 in Endometrial Cancer on the mRNA and Protein Level
title_full Assessment of Expression of Homeobox A5 in Endometrial Cancer on the mRNA and Protein Level
title_fullStr Assessment of Expression of Homeobox A5 in Endometrial Cancer on the mRNA and Protein Level
title_full_unstemmed Assessment of Expression of Homeobox A5 in Endometrial Cancer on the mRNA and Protein Level
title_short Assessment of Expression of Homeobox A5 in Endometrial Cancer on the mRNA and Protein Level
title_sort assessment of expression of homeobox a5 in endometrial cancer on the mrna and protein level
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403687/
https://www.ncbi.nlm.nih.gov/pubmed/31880256
http://dx.doi.org/10.2174/1389201021666191227121627
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