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TLR4 896A/G and TLR9 1174G/A polymorphisms are associated with the risk of infectious mononucleosis
Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and activate innate and adaptive immune responses. Single nucleotide polymorphisms (SNPs) within the TLR genes may influence host–pathogen interactions and can have an impact on the progression of infectious diseases. The pr...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
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Nature Publishing Group UK
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403730/ https://www.ncbi.nlm.nih.gov/pubmed/32753695 http://dx.doi.org/10.1038/s41598-020-70129-4 |
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author | Jabłońska, Agnieszka Studzińska, Mirosława Szenborn, Leszek Wiśniewska-Ligier, Małgorzata Karlikowska-Skwarnik, Monika Gęsicki, Tomasz Paradowska, Edyta |
author_facet | Jabłońska, Agnieszka Studzińska, Mirosława Szenborn, Leszek Wiśniewska-Ligier, Małgorzata Karlikowska-Skwarnik, Monika Gęsicki, Tomasz Paradowska, Edyta |
author_sort | Jabłońska, Agnieszka |
collection | PubMed |
description | Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and activate innate and adaptive immune responses. Single nucleotide polymorphisms (SNPs) within the TLR genes may influence host–pathogen interactions and can have an impact on the progression of infectious diseases. The present study aimed to investigate the genotype distribution of TLR2 (2029C/T, rs121917864; 2258G/A, rs5743708), TLR4 (896A/G, rs4986790), and TLR9 (− 1237T/C, rs5743836; − 1486T/C, rs187084; 1174G/A, rs352139; and 2848C/T, rs352140) polymorphisms in 149 children and adolescents with infectious mononucleosis (IM) and 140 healthy individuals. The potential association of TLR SNPs with the clinical manifestations of EBV infection was also studied. The presence of TLR2, TLR4, and TLR9 SNPs was identified by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP). EBV DNA loads were detected by quantitative real-time PCR assay. The TLR4 896 GG and the TLR9 1174 GA genotypes were associated with an increased risk of EBV-related IM in examined patients (p = 0.014 and p = 0.001, respectively). The heterozygous genotype of the TLR4 896A/G SNP was associated with an increased risk of elevated liver enzyme levels and leukocytosis (p < 0.05). Our preliminary study revealed that the TLR4 896A/G and the TLR9 1174G/A polymorphisms seem to be related to the course of acute EBV infection in children and adolescents. |
format | Online Article Text |
id | pubmed-7403730 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Nature Publishing Group UK |
record_format | MEDLINE/PubMed |
spelling | pubmed-74037302020-08-07 TLR4 896A/G and TLR9 1174G/A polymorphisms are associated with the risk of infectious mononucleosis Jabłońska, Agnieszka Studzińska, Mirosława Szenborn, Leszek Wiśniewska-Ligier, Małgorzata Karlikowska-Skwarnik, Monika Gęsicki, Tomasz Paradowska, Edyta Sci Rep Article Toll-like receptors (TLRs) recognize pathogen-associated molecular patterns and activate innate and adaptive immune responses. Single nucleotide polymorphisms (SNPs) within the TLR genes may influence host–pathogen interactions and can have an impact on the progression of infectious diseases. The present study aimed to investigate the genotype distribution of TLR2 (2029C/T, rs121917864; 2258G/A, rs5743708), TLR4 (896A/G, rs4986790), and TLR9 (− 1237T/C, rs5743836; − 1486T/C, rs187084; 1174G/A, rs352139; and 2848C/T, rs352140) polymorphisms in 149 children and adolescents with infectious mononucleosis (IM) and 140 healthy individuals. The potential association of TLR SNPs with the clinical manifestations of EBV infection was also studied. The presence of TLR2, TLR4, and TLR9 SNPs was identified by polymerase chain reaction–restriction fragment length polymorphism (PCR–RFLP). EBV DNA loads were detected by quantitative real-time PCR assay. The TLR4 896 GG and the TLR9 1174 GA genotypes were associated with an increased risk of EBV-related IM in examined patients (p = 0.014 and p = 0.001, respectively). The heterozygous genotype of the TLR4 896A/G SNP was associated with an increased risk of elevated liver enzyme levels and leukocytosis (p < 0.05). Our preliminary study revealed that the TLR4 896A/G and the TLR9 1174G/A polymorphisms seem to be related to the course of acute EBV infection in children and adolescents. Nature Publishing Group UK 2020-08-04 /pmc/articles/PMC7403730/ /pubmed/32753695 http://dx.doi.org/10.1038/s41598-020-70129-4 Text en © The Author(s) 2020 Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/. |
spellingShingle | Article Jabłońska, Agnieszka Studzińska, Mirosława Szenborn, Leszek Wiśniewska-Ligier, Małgorzata Karlikowska-Skwarnik, Monika Gęsicki, Tomasz Paradowska, Edyta TLR4 896A/G and TLR9 1174G/A polymorphisms are associated with the risk of infectious mononucleosis |
title | TLR4 896A/G and TLR9 1174G/A polymorphisms are associated with the risk of infectious mononucleosis |
title_full | TLR4 896A/G and TLR9 1174G/A polymorphisms are associated with the risk of infectious mononucleosis |
title_fullStr | TLR4 896A/G and TLR9 1174G/A polymorphisms are associated with the risk of infectious mononucleosis |
title_full_unstemmed | TLR4 896A/G and TLR9 1174G/A polymorphisms are associated with the risk of infectious mononucleosis |
title_short | TLR4 896A/G and TLR9 1174G/A polymorphisms are associated with the risk of infectious mononucleosis |
title_sort | tlr4 896a/g and tlr9 1174g/a polymorphisms are associated with the risk of infectious mononucleosis |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403730/ https://www.ncbi.nlm.nih.gov/pubmed/32753695 http://dx.doi.org/10.1038/s41598-020-70129-4 |
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