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Transcriptome Analysis Provides Insights into Grain Filling in Foxtail Millet (Setaria italica L.)

Grain filling is an importantly developmental process which is associated with the yield and quality of foxtail millet (Setaria italic L.). However, the molecular mechanisms of grain filling are rarely reported in foxtail millet. In our study, RNA-seq was performed to investigate the transcriptional...

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Autores principales: Wang, Tao, Song, Hui, Li, Pengtao, Wei, Yangyang, Hu, Nan, Chen, Zhenwen, Wang, Weiqi, Liu, Jinrong, Zhang, Baohong, Peng, Renhai
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403974/
https://www.ncbi.nlm.nih.gov/pubmed/32708737
http://dx.doi.org/10.3390/ijms21145031
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author Wang, Tao
Song, Hui
Li, Pengtao
Wei, Yangyang
Hu, Nan
Chen, Zhenwen
Wang, Weiqi
Liu, Jinrong
Zhang, Baohong
Peng, Renhai
author_facet Wang, Tao
Song, Hui
Li, Pengtao
Wei, Yangyang
Hu, Nan
Chen, Zhenwen
Wang, Weiqi
Liu, Jinrong
Zhang, Baohong
Peng, Renhai
author_sort Wang, Tao
collection PubMed
description Grain filling is an importantly developmental process which is associated with the yield and quality of foxtail millet (Setaria italic L.). However, the molecular mechanisms of grain filling are rarely reported in foxtail millet. In our study, RNA-seq was performed to investigate the transcriptional dynamics and identify the key genes involved in grain filling in foxtail millet at five different developmental stages. A total of 11,399 differentially expressed genes (DEGs), including 902 transcription factors (TFs), were identified. Certain important genes involved in grain filling were discovered through a function annotation and temporal expression patterns analysis. These genes included genes associated with starch biosynthesis, cell-wall invertases, hormone signal transduction, and polyamine metabolism pathways. The expression levels of seven randomly selected DEGs were validated by a quantitative real-time polymerase chain reaction (qRT-PCR). This study provides the first insight into the changes in the gene expression of grain filling at different developmental stages in foxtail millet. These results could help understand the complex molecular mechanisms of the panicle formation in foxtail millet and other cereal crops.
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spelling pubmed-74039742020-08-11 Transcriptome Analysis Provides Insights into Grain Filling in Foxtail Millet (Setaria italica L.) Wang, Tao Song, Hui Li, Pengtao Wei, Yangyang Hu, Nan Chen, Zhenwen Wang, Weiqi Liu, Jinrong Zhang, Baohong Peng, Renhai Int J Mol Sci Article Grain filling is an importantly developmental process which is associated with the yield and quality of foxtail millet (Setaria italic L.). However, the molecular mechanisms of grain filling are rarely reported in foxtail millet. In our study, RNA-seq was performed to investigate the transcriptional dynamics and identify the key genes involved in grain filling in foxtail millet at five different developmental stages. A total of 11,399 differentially expressed genes (DEGs), including 902 transcription factors (TFs), were identified. Certain important genes involved in grain filling were discovered through a function annotation and temporal expression patterns analysis. These genes included genes associated with starch biosynthesis, cell-wall invertases, hormone signal transduction, and polyamine metabolism pathways. The expression levels of seven randomly selected DEGs were validated by a quantitative real-time polymerase chain reaction (qRT-PCR). This study provides the first insight into the changes in the gene expression of grain filling at different developmental stages in foxtail millet. These results could help understand the complex molecular mechanisms of the panicle formation in foxtail millet and other cereal crops. MDPI 2020-07-16 /pmc/articles/PMC7403974/ /pubmed/32708737 http://dx.doi.org/10.3390/ijms21145031 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Wang, Tao
Song, Hui
Li, Pengtao
Wei, Yangyang
Hu, Nan
Chen, Zhenwen
Wang, Weiqi
Liu, Jinrong
Zhang, Baohong
Peng, Renhai
Transcriptome Analysis Provides Insights into Grain Filling in Foxtail Millet (Setaria italica L.)
title Transcriptome Analysis Provides Insights into Grain Filling in Foxtail Millet (Setaria italica L.)
title_full Transcriptome Analysis Provides Insights into Grain Filling in Foxtail Millet (Setaria italica L.)
title_fullStr Transcriptome Analysis Provides Insights into Grain Filling in Foxtail Millet (Setaria italica L.)
title_full_unstemmed Transcriptome Analysis Provides Insights into Grain Filling in Foxtail Millet (Setaria italica L.)
title_short Transcriptome Analysis Provides Insights into Grain Filling in Foxtail Millet (Setaria italica L.)
title_sort transcriptome analysis provides insights into grain filling in foxtail millet (setaria italica l.)
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7403974/
https://www.ncbi.nlm.nih.gov/pubmed/32708737
http://dx.doi.org/10.3390/ijms21145031
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