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Unproductive Effects of ALK Gene Amplification and Copy Number Gain in Non-Small-Cell Lung Cancer. ALK Gene Amplification and Copy Gain in NSCLC

Background: The Anaplastic Lymphoma Kinase (ALK) gene is known to be affected by several genetic alterations, such as rearrangement, amplification and point mutation. The main goal of this study was to comprehensively analyze ALK amplification (ALK-A) and ALK gene copy number gain (ALK-CNG) in a lar...

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Autores principales: Zito Marino, Federica, Botti, Gerardo, Aquino, Gabriella, Ferrero, Stefano, Gaudioso, Gabriella, Palleschi, Alessandro, Rocco, Danilo, Salvi, Rosario, Micheli, Maria Carolina, Micheli, Pietro, Morabito, Alessandro, Rocco, Gaetano, Giordano, Antonio, De Cecio, Rossella, Franco, Renato
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404032/
https://www.ncbi.nlm.nih.gov/pubmed/32664698
http://dx.doi.org/10.3390/ijms21144927
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author Zito Marino, Federica
Botti, Gerardo
Aquino, Gabriella
Ferrero, Stefano
Gaudioso, Gabriella
Palleschi, Alessandro
Rocco, Danilo
Salvi, Rosario
Micheli, Maria Carolina
Micheli, Pietro
Morabito, Alessandro
Rocco, Gaetano
Giordano, Antonio
De Cecio, Rossella
Franco, Renato
author_facet Zito Marino, Federica
Botti, Gerardo
Aquino, Gabriella
Ferrero, Stefano
Gaudioso, Gabriella
Palleschi, Alessandro
Rocco, Danilo
Salvi, Rosario
Micheli, Maria Carolina
Micheli, Pietro
Morabito, Alessandro
Rocco, Gaetano
Giordano, Antonio
De Cecio, Rossella
Franco, Renato
author_sort Zito Marino, Federica
collection PubMed
description Background: The Anaplastic Lymphoma Kinase (ALK) gene is known to be affected by several genetic alterations, such as rearrangement, amplification and point mutation. The main goal of this study was to comprehensively analyze ALK amplification (ALK-A) and ALK gene copy number gain (ALK-CNG) in a large cohort of non-small-cell lung cancer (NSCLC) patients in order to evaluate the effects on mRNA and protein expression. Methods: ALK locus number status was evaluated in 578 NSCLC cases by fluorescence in situ hybridization (FISH). In addition, ALK immunohistochemistry and ALK mRNA in situ hybridization were performed. Results: Out of 578 cases, 17 cases showed ALK-A. In addition, 14 cases presented ALK-CNG and 72 cases presented chromosome 2 polyploidy. None of those carrying ALK-A and -CNG showed either ALK immunohistochemical expression or ALK mRNA expression through in situ hybridization. We observed a high frequency of extra copies of the ALK gene. Conclusions: Our findings demonstrated that ALK-A is not involved in mRNA production and consequently is not involved in protein production; these findings support the hypothesis that ALK-A might not play a role in the pathogenesis of NSCLC, underlining the absence of a specific clinical application.
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spelling pubmed-74040322020-08-11 Unproductive Effects of ALK Gene Amplification and Copy Number Gain in Non-Small-Cell Lung Cancer. ALK Gene Amplification and Copy Gain in NSCLC Zito Marino, Federica Botti, Gerardo Aquino, Gabriella Ferrero, Stefano Gaudioso, Gabriella Palleschi, Alessandro Rocco, Danilo Salvi, Rosario Micheli, Maria Carolina Micheli, Pietro Morabito, Alessandro Rocco, Gaetano Giordano, Antonio De Cecio, Rossella Franco, Renato Int J Mol Sci Article Background: The Anaplastic Lymphoma Kinase (ALK) gene is known to be affected by several genetic alterations, such as rearrangement, amplification and point mutation. The main goal of this study was to comprehensively analyze ALK amplification (ALK-A) and ALK gene copy number gain (ALK-CNG) in a large cohort of non-small-cell lung cancer (NSCLC) patients in order to evaluate the effects on mRNA and protein expression. Methods: ALK locus number status was evaluated in 578 NSCLC cases by fluorescence in situ hybridization (FISH). In addition, ALK immunohistochemistry and ALK mRNA in situ hybridization were performed. Results: Out of 578 cases, 17 cases showed ALK-A. In addition, 14 cases presented ALK-CNG and 72 cases presented chromosome 2 polyploidy. None of those carrying ALK-A and -CNG showed either ALK immunohistochemical expression or ALK mRNA expression through in situ hybridization. We observed a high frequency of extra copies of the ALK gene. Conclusions: Our findings demonstrated that ALK-A is not involved in mRNA production and consequently is not involved in protein production; these findings support the hypothesis that ALK-A might not play a role in the pathogenesis of NSCLC, underlining the absence of a specific clinical application. MDPI 2020-07-12 /pmc/articles/PMC7404032/ /pubmed/32664698 http://dx.doi.org/10.3390/ijms21144927 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Zito Marino, Federica
Botti, Gerardo
Aquino, Gabriella
Ferrero, Stefano
Gaudioso, Gabriella
Palleschi, Alessandro
Rocco, Danilo
Salvi, Rosario
Micheli, Maria Carolina
Micheli, Pietro
Morabito, Alessandro
Rocco, Gaetano
Giordano, Antonio
De Cecio, Rossella
Franco, Renato
Unproductive Effects of ALK Gene Amplification and Copy Number Gain in Non-Small-Cell Lung Cancer. ALK Gene Amplification and Copy Gain in NSCLC
title Unproductive Effects of ALK Gene Amplification and Copy Number Gain in Non-Small-Cell Lung Cancer. ALK Gene Amplification and Copy Gain in NSCLC
title_full Unproductive Effects of ALK Gene Amplification and Copy Number Gain in Non-Small-Cell Lung Cancer. ALK Gene Amplification and Copy Gain in NSCLC
title_fullStr Unproductive Effects of ALK Gene Amplification and Copy Number Gain in Non-Small-Cell Lung Cancer. ALK Gene Amplification and Copy Gain in NSCLC
title_full_unstemmed Unproductive Effects of ALK Gene Amplification and Copy Number Gain in Non-Small-Cell Lung Cancer. ALK Gene Amplification and Copy Gain in NSCLC
title_short Unproductive Effects of ALK Gene Amplification and Copy Number Gain in Non-Small-Cell Lung Cancer. ALK Gene Amplification and Copy Gain in NSCLC
title_sort unproductive effects of alk gene amplification and copy number gain in non-small-cell lung cancer. alk gene amplification and copy gain in nsclc
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404032/
https://www.ncbi.nlm.nih.gov/pubmed/32664698
http://dx.doi.org/10.3390/ijms21144927
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