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Non-Thermal Plasma Application in Tumor-Bearing Mice Induces Increase of Serum HMGB1

The application of cold atmospheric plasma (CAP) in cancer therapy could be one of the new anticancer strategies. In the current work, we used cold atmospheric plasma jet for the treatment of cultured cells and mice. We showed that CAP induced the death of MX−7 mouse rhabdomyosarcoma cells with the...

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Autores principales: Troitskaya, Olga, Golubitskaya, Ekaterina, Biryukov, Mikhail, Varlamov, Mikhail, Gugin, Pavel, Milakhina, Elena, Richter, Vladimir, Schweigert, Irina, Zakrevsky, Dmitry, Koval, Olga
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404183/
https://www.ncbi.nlm.nih.gov/pubmed/32698492
http://dx.doi.org/10.3390/ijms21145128
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author Troitskaya, Olga
Golubitskaya, Ekaterina
Biryukov, Mikhail
Varlamov, Mikhail
Gugin, Pavel
Milakhina, Elena
Richter, Vladimir
Schweigert, Irina
Zakrevsky, Dmitry
Koval, Olga
author_facet Troitskaya, Olga
Golubitskaya, Ekaterina
Biryukov, Mikhail
Varlamov, Mikhail
Gugin, Pavel
Milakhina, Elena
Richter, Vladimir
Schweigert, Irina
Zakrevsky, Dmitry
Koval, Olga
author_sort Troitskaya, Olga
collection PubMed
description The application of cold atmospheric plasma (CAP) in cancer therapy could be one of the new anticancer strategies. In the current work, we used cold atmospheric plasma jet for the treatment of cultured cells and mice. We showed that CAP induced the death of MX−7 mouse rhabdomyosarcoma cells with the hallmarks of immunogenic cell death (ICD): calreticulin and heat shock protein 70 (HSP70) externalization and high-mobility group box 1 protein (HMGB1) release. The intensity of HMGB1 release after the CAP treatment correlated directly with the basal extracellular HMGB1 level. Releasing from dying cells, HMGB1 can act as a proinflammatory cytokine. Our in vivo study demonstrated that cold atmospheric plasma induces a short-term two-times increase in serum HMGB1 level only in tumor-bearing mice with no effect in healthy mice. These findings support our hypothesis that CAP-dependent HMGB1 release from dying cancer cells can change the serum HMGB1 level. At the same time, we showed a weak cytokine response to CAP irradiation in healthy mice that can characterize CAP as an immune-safety physical antitumor approach.
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spelling pubmed-74041832020-08-11 Non-Thermal Plasma Application in Tumor-Bearing Mice Induces Increase of Serum HMGB1 Troitskaya, Olga Golubitskaya, Ekaterina Biryukov, Mikhail Varlamov, Mikhail Gugin, Pavel Milakhina, Elena Richter, Vladimir Schweigert, Irina Zakrevsky, Dmitry Koval, Olga Int J Mol Sci Article The application of cold atmospheric plasma (CAP) in cancer therapy could be one of the new anticancer strategies. In the current work, we used cold atmospheric plasma jet for the treatment of cultured cells and mice. We showed that CAP induced the death of MX−7 mouse rhabdomyosarcoma cells with the hallmarks of immunogenic cell death (ICD): calreticulin and heat shock protein 70 (HSP70) externalization and high-mobility group box 1 protein (HMGB1) release. The intensity of HMGB1 release after the CAP treatment correlated directly with the basal extracellular HMGB1 level. Releasing from dying cells, HMGB1 can act as a proinflammatory cytokine. Our in vivo study demonstrated that cold atmospheric plasma induces a short-term two-times increase in serum HMGB1 level only in tumor-bearing mice with no effect in healthy mice. These findings support our hypothesis that CAP-dependent HMGB1 release from dying cancer cells can change the serum HMGB1 level. At the same time, we showed a weak cytokine response to CAP irradiation in healthy mice that can characterize CAP as an immune-safety physical antitumor approach. MDPI 2020-07-20 /pmc/articles/PMC7404183/ /pubmed/32698492 http://dx.doi.org/10.3390/ijms21145128 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Troitskaya, Olga
Golubitskaya, Ekaterina
Biryukov, Mikhail
Varlamov, Mikhail
Gugin, Pavel
Milakhina, Elena
Richter, Vladimir
Schweigert, Irina
Zakrevsky, Dmitry
Koval, Olga
Non-Thermal Plasma Application in Tumor-Bearing Mice Induces Increase of Serum HMGB1
title Non-Thermal Plasma Application in Tumor-Bearing Mice Induces Increase of Serum HMGB1
title_full Non-Thermal Plasma Application in Tumor-Bearing Mice Induces Increase of Serum HMGB1
title_fullStr Non-Thermal Plasma Application in Tumor-Bearing Mice Induces Increase of Serum HMGB1
title_full_unstemmed Non-Thermal Plasma Application in Tumor-Bearing Mice Induces Increase of Serum HMGB1
title_short Non-Thermal Plasma Application in Tumor-Bearing Mice Induces Increase of Serum HMGB1
title_sort non-thermal plasma application in tumor-bearing mice induces increase of serum hmgb1
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404183/
https://www.ncbi.nlm.nih.gov/pubmed/32698492
http://dx.doi.org/10.3390/ijms21145128
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