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Anti-CXCL4 Antibody Reactivity Is Present in Systemic Sclerosis (SSc) and Correlates with the SSc Type I Interferon Signature

Systemic sclerosis (SSc) is characterized by skin/internal organ fibrosis, vasculopathy and autoimmunity. Chemokine (C-X-C motif) ligand 4 (CXCL4) is an SSc biomarker, predicting unfavorable prognosis and lung fibrosis. CXCL4 binds DNA/RNA and favors interferon (IFN)-α production by plasmacytoid den...

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Autores principales: Lande, Roberto, Mennella, Anna, Palazzo, Raffaella, Pietraforte, Immacolata, Stefanantoni, Katia, Iannace, Nicoletta, Butera, Alessia, Boirivant, Monica, Pica, Roberta, Conrad, Curdin, Chizzolini, Carlo, Riccieri, Valeria, Frasca, Loredana
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404208/
https://www.ncbi.nlm.nih.gov/pubmed/32707718
http://dx.doi.org/10.3390/ijms21145102
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author Lande, Roberto
Mennella, Anna
Palazzo, Raffaella
Pietraforte, Immacolata
Stefanantoni, Katia
Iannace, Nicoletta
Butera, Alessia
Boirivant, Monica
Pica, Roberta
Conrad, Curdin
Chizzolini, Carlo
Riccieri, Valeria
Frasca, Loredana
author_facet Lande, Roberto
Mennella, Anna
Palazzo, Raffaella
Pietraforte, Immacolata
Stefanantoni, Katia
Iannace, Nicoletta
Butera, Alessia
Boirivant, Monica
Pica, Roberta
Conrad, Curdin
Chizzolini, Carlo
Riccieri, Valeria
Frasca, Loredana
author_sort Lande, Roberto
collection PubMed
description Systemic sclerosis (SSc) is characterized by skin/internal organ fibrosis, vasculopathy and autoimmunity. Chemokine (C-X-C motif) ligand 4 (CXCL4) is an SSc biomarker, predicting unfavorable prognosis and lung fibrosis. CXCL4 binds DNA/RNA and favors interferon (IFN)-α production by plasmacytoid dendritic cells (pDCs), contributing to the type I IFN (IFN-I) signature in SSc patients. However, whether CXCL4 is an autoantigen in SSc is unknown. Here, we show that at least half of SSc patients show consistent antibody reactivity to CXCL4. T-cell proliferation to CXCL4, tested in a limited number of patients, correlates with anti-CXCL4 antibody reactivity. Antibodies to CXCL4 mostly correlate with circulating IFN-α levels and are significantly higher in patients with lung fibrosis in two independent SSc cohorts. Antibodies to CXCL4 implement the CXCL4–DNA complex’s effect on IFN-α production by pDCs; CXCL4–DNA/RNA complexes stimulate purified human B-cells to become antibody-secreting plasma cells in vitro. These data indicate that CXCL4 is indeed an autoantigen in SSc and suggest that CXCL4, and CXCL4-specific autoantibodies, can fuel a harmful loop: CXCL4–DNA/RNA complexes induce IFN-α in pDCs and direct B-cell stimulation, including the secretion of anti-CXCL4 antibodies. Anti-CXCL4 antibodies may further increase pDC stimulation and IFN-α release in vivo, creating a vicious cycle which sustains the SSc IFN-I signature and general inflammation.
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spelling pubmed-74042082020-08-11 Anti-CXCL4 Antibody Reactivity Is Present in Systemic Sclerosis (SSc) and Correlates with the SSc Type I Interferon Signature Lande, Roberto Mennella, Anna Palazzo, Raffaella Pietraforte, Immacolata Stefanantoni, Katia Iannace, Nicoletta Butera, Alessia Boirivant, Monica Pica, Roberta Conrad, Curdin Chizzolini, Carlo Riccieri, Valeria Frasca, Loredana Int J Mol Sci Article Systemic sclerosis (SSc) is characterized by skin/internal organ fibrosis, vasculopathy and autoimmunity. Chemokine (C-X-C motif) ligand 4 (CXCL4) is an SSc biomarker, predicting unfavorable prognosis and lung fibrosis. CXCL4 binds DNA/RNA and favors interferon (IFN)-α production by plasmacytoid dendritic cells (pDCs), contributing to the type I IFN (IFN-I) signature in SSc patients. However, whether CXCL4 is an autoantigen in SSc is unknown. Here, we show that at least half of SSc patients show consistent antibody reactivity to CXCL4. T-cell proliferation to CXCL4, tested in a limited number of patients, correlates with anti-CXCL4 antibody reactivity. Antibodies to CXCL4 mostly correlate with circulating IFN-α levels and are significantly higher in patients with lung fibrosis in two independent SSc cohorts. Antibodies to CXCL4 implement the CXCL4–DNA complex’s effect on IFN-α production by pDCs; CXCL4–DNA/RNA complexes stimulate purified human B-cells to become antibody-secreting plasma cells in vitro. These data indicate that CXCL4 is indeed an autoantigen in SSc and suggest that CXCL4, and CXCL4-specific autoantibodies, can fuel a harmful loop: CXCL4–DNA/RNA complexes induce IFN-α in pDCs and direct B-cell stimulation, including the secretion of anti-CXCL4 antibodies. Anti-CXCL4 antibodies may further increase pDC stimulation and IFN-α release in vivo, creating a vicious cycle which sustains the SSc IFN-I signature and general inflammation. MDPI 2020-07-19 /pmc/articles/PMC7404208/ /pubmed/32707718 http://dx.doi.org/10.3390/ijms21145102 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Lande, Roberto
Mennella, Anna
Palazzo, Raffaella
Pietraforte, Immacolata
Stefanantoni, Katia
Iannace, Nicoletta
Butera, Alessia
Boirivant, Monica
Pica, Roberta
Conrad, Curdin
Chizzolini, Carlo
Riccieri, Valeria
Frasca, Loredana
Anti-CXCL4 Antibody Reactivity Is Present in Systemic Sclerosis (SSc) and Correlates with the SSc Type I Interferon Signature
title Anti-CXCL4 Antibody Reactivity Is Present in Systemic Sclerosis (SSc) and Correlates with the SSc Type I Interferon Signature
title_full Anti-CXCL4 Antibody Reactivity Is Present in Systemic Sclerosis (SSc) and Correlates with the SSc Type I Interferon Signature
title_fullStr Anti-CXCL4 Antibody Reactivity Is Present in Systemic Sclerosis (SSc) and Correlates with the SSc Type I Interferon Signature
title_full_unstemmed Anti-CXCL4 Antibody Reactivity Is Present in Systemic Sclerosis (SSc) and Correlates with the SSc Type I Interferon Signature
title_short Anti-CXCL4 Antibody Reactivity Is Present in Systemic Sclerosis (SSc) and Correlates with the SSc Type I Interferon Signature
title_sort anti-cxcl4 antibody reactivity is present in systemic sclerosis (ssc) and correlates with the ssc type i interferon signature
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404208/
https://www.ncbi.nlm.nih.gov/pubmed/32707718
http://dx.doi.org/10.3390/ijms21145102
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