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Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes
[Image: see text] Strategies for endosomal escape and access to the cell nucleus are highly sought for nanocarriers to deliver their load efficiently following endocytosis. In this work, we have studied the uptake and intracellular trafficking of a polycationic polyamidoamine (PAA) endowed with a lu...
Autores principales: | , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
American
Chemical Society
2020
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Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404250/ https://www.ncbi.nlm.nih.gov/pubmed/32643926 http://dx.doi.org/10.1021/acsami.0c08033 |
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author | Mascheroni, Luca Francia, Valentina Rossotti, Beatrice Ranucci, Elisabetta Ferruti, Paolo Maggioni, Daniela Salvati, Anna |
author_facet | Mascheroni, Luca Francia, Valentina Rossotti, Beatrice Ranucci, Elisabetta Ferruti, Paolo Maggioni, Daniela Salvati, Anna |
author_sort | Mascheroni, Luca |
collection | PubMed |
description | [Image: see text] Strategies for endosomal escape and access to the cell nucleus are highly sought for nanocarriers to deliver their load efficiently following endocytosis. In this work, we have studied the uptake and intracellular trafficking of a polycationic polyamidoamine (PAA) endowed with a luminescent Ru complex, Ru–PhenAN, that shows unique trafficking to the cell nucleus. Live cell imaging confirmed the capacity of this polymer to access the nucleus, excluding artifacts due to cell fixation, and clarified that the mechanism of escape is light-triggered and relies on the presence of the Ru complexes and their capacity to absorb light and act as photosensitizers for singlet oxygen production. These results open up the possibility to use PAA–ruthenium complexes for targeted light-triggered delivery of genetic material or drugs to the cytosol and nucleus. |
format | Online Article Text |
id | pubmed-7404250 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | American
Chemical Society |
record_format | MEDLINE/PubMed |
spelling | pubmed-74042502020-08-06 Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes Mascheroni, Luca Francia, Valentina Rossotti, Beatrice Ranucci, Elisabetta Ferruti, Paolo Maggioni, Daniela Salvati, Anna ACS Appl Mater Interfaces [Image: see text] Strategies for endosomal escape and access to the cell nucleus are highly sought for nanocarriers to deliver their load efficiently following endocytosis. In this work, we have studied the uptake and intracellular trafficking of a polycationic polyamidoamine (PAA) endowed with a luminescent Ru complex, Ru–PhenAN, that shows unique trafficking to the cell nucleus. Live cell imaging confirmed the capacity of this polymer to access the nucleus, excluding artifacts due to cell fixation, and clarified that the mechanism of escape is light-triggered and relies on the presence of the Ru complexes and their capacity to absorb light and act as photosensitizers for singlet oxygen production. These results open up the possibility to use PAA–ruthenium complexes for targeted light-triggered delivery of genetic material or drugs to the cytosol and nucleus. American Chemical Society 2020-07-09 2020-08-05 /pmc/articles/PMC7404250/ /pubmed/32643926 http://dx.doi.org/10.1021/acsami.0c08033 Text en Copyright © 2020 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes. |
spellingShingle | Mascheroni, Luca Francia, Valentina Rossotti, Beatrice Ranucci, Elisabetta Ferruti, Paolo Maggioni, Daniela Salvati, Anna Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes |
title | Light-Triggered
Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized
with Ruthenium Complexes |
title_full | Light-Triggered
Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized
with Ruthenium Complexes |
title_fullStr | Light-Triggered
Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized
with Ruthenium Complexes |
title_full_unstemmed | Light-Triggered
Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized
with Ruthenium Complexes |
title_short | Light-Triggered
Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized
with Ruthenium Complexes |
title_sort | light-triggered
trafficking to the cell nucleus of a cationic polyamidoamine functionalized
with ruthenium complexes |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404250/ https://www.ncbi.nlm.nih.gov/pubmed/32643926 http://dx.doi.org/10.1021/acsami.0c08033 |
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