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Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes

[Image: see text] Strategies for endosomal escape and access to the cell nucleus are highly sought for nanocarriers to deliver their load efficiently following endocytosis. In this work, we have studied the uptake and intracellular trafficking of a polycationic polyamidoamine (PAA) endowed with a lu...

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Autores principales: Mascheroni, Luca, Francia, Valentina, Rossotti, Beatrice, Ranucci, Elisabetta, Ferruti, Paolo, Maggioni, Daniela, Salvati, Anna
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Chemical Society 2020
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404250/
https://www.ncbi.nlm.nih.gov/pubmed/32643926
http://dx.doi.org/10.1021/acsami.0c08033
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author Mascheroni, Luca
Francia, Valentina
Rossotti, Beatrice
Ranucci, Elisabetta
Ferruti, Paolo
Maggioni, Daniela
Salvati, Anna
author_facet Mascheroni, Luca
Francia, Valentina
Rossotti, Beatrice
Ranucci, Elisabetta
Ferruti, Paolo
Maggioni, Daniela
Salvati, Anna
author_sort Mascheroni, Luca
collection PubMed
description [Image: see text] Strategies for endosomal escape and access to the cell nucleus are highly sought for nanocarriers to deliver their load efficiently following endocytosis. In this work, we have studied the uptake and intracellular trafficking of a polycationic polyamidoamine (PAA) endowed with a luminescent Ru complex, Ru–PhenAN, that shows unique trafficking to the cell nucleus. Live cell imaging confirmed the capacity of this polymer to access the nucleus, excluding artifacts due to cell fixation, and clarified that the mechanism of escape is light-triggered and relies on the presence of the Ru complexes and their capacity to absorb light and act as photosensitizers for singlet oxygen production. These results open up the possibility to use PAA–ruthenium complexes for targeted light-triggered delivery of genetic material or drugs to the cytosol and nucleus.
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spelling pubmed-74042502020-08-06 Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes Mascheroni, Luca Francia, Valentina Rossotti, Beatrice Ranucci, Elisabetta Ferruti, Paolo Maggioni, Daniela Salvati, Anna ACS Appl Mater Interfaces [Image: see text] Strategies for endosomal escape and access to the cell nucleus are highly sought for nanocarriers to deliver their load efficiently following endocytosis. In this work, we have studied the uptake and intracellular trafficking of a polycationic polyamidoamine (PAA) endowed with a luminescent Ru complex, Ru–PhenAN, that shows unique trafficking to the cell nucleus. Live cell imaging confirmed the capacity of this polymer to access the nucleus, excluding artifacts due to cell fixation, and clarified that the mechanism of escape is light-triggered and relies on the presence of the Ru complexes and their capacity to absorb light and act as photosensitizers for singlet oxygen production. These results open up the possibility to use PAA–ruthenium complexes for targeted light-triggered delivery of genetic material or drugs to the cytosol and nucleus. American Chemical Society 2020-07-09 2020-08-05 /pmc/articles/PMC7404250/ /pubmed/32643926 http://dx.doi.org/10.1021/acsami.0c08033 Text en Copyright © 2020 American Chemical Society This is an open access article published under a Creative Commons Non-Commercial No Derivative Works (CC-BY-NC-ND) Attribution License (http://pubs.acs.org/page/policy/authorchoice_ccbyncnd_termsofuse.html) , which permits copying and redistribution of the article, and creation of adaptations, all for non-commercial purposes.
spellingShingle Mascheroni, Luca
Francia, Valentina
Rossotti, Beatrice
Ranucci, Elisabetta
Ferruti, Paolo
Maggioni, Daniela
Salvati, Anna
Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes
title Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes
title_full Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes
title_fullStr Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes
title_full_unstemmed Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes
title_short Light-Triggered Trafficking to the Cell Nucleus of a Cationic Polyamidoamine Functionalized with Ruthenium Complexes
title_sort light-triggered trafficking to the cell nucleus of a cationic polyamidoamine functionalized with ruthenium complexes
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404250/
https://www.ncbi.nlm.nih.gov/pubmed/32643926
http://dx.doi.org/10.1021/acsami.0c08033
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