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Sperm Motility of Mice under Simulated Microgravity and Hypergravity

For deep space exploration, reproductive health must be maintained to preserve the species. However, the mechanisms underlying the effect of changes in gravity on male germ cells remain poorly understood. The aim of this study was to determine the effect of simulated micro- and hypergravity on mouse...

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Autores principales: Ogneva, Irina V., Usik, Maria A., Biryukov, Nikolay S., Zhdankina, Yuliya S.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404272/
https://www.ncbi.nlm.nih.gov/pubmed/32709012
http://dx.doi.org/10.3390/ijms21145054
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author Ogneva, Irina V.
Usik, Maria A.
Biryukov, Nikolay S.
Zhdankina, Yuliya S.
author_facet Ogneva, Irina V.
Usik, Maria A.
Biryukov, Nikolay S.
Zhdankina, Yuliya S.
author_sort Ogneva, Irina V.
collection PubMed
description For deep space exploration, reproductive health must be maintained to preserve the species. However, the mechanisms underlying the effect of changes in gravity on male germ cells remain poorly understood. The aim of this study was to determine the effect of simulated micro- and hypergravity on mouse sperm motility and the mechanisms of this change. For 1, 3 and 6 h, mouse sperm samples isolated from the caudal epididymis were subjected to simulated microgravity using a random position machine and 2g hypergravity using a centrifuge. The experimental samples were compared with static and dynamic controls. The sperm motility and the percentage of motile sperm were determined using microscopy and video analysis, cell respiration was determined by polarography, the protein content was assessed by Western blotting and the mRNA levels were determined using qRT-PCR. The results indicated that hypergravity conditions led to more significant changes than simulated microgravity conditions: after 1 h, the speed of sperm movement decreased, and after 3 h, the number of motile cells began to decrease. Under the microgravity model, the speed of movement did not change, but the motile spermatozoa decreased after 6 h of exposure. These changes are likely associated with a change in the structure of the microtubule cytoskeleton, and changes in the energy supply are an adaptive reaction to changes in sperm motility.
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spelling pubmed-74042722020-08-18 Sperm Motility of Mice under Simulated Microgravity and Hypergravity Ogneva, Irina V. Usik, Maria A. Biryukov, Nikolay S. Zhdankina, Yuliya S. Int J Mol Sci Article For deep space exploration, reproductive health must be maintained to preserve the species. However, the mechanisms underlying the effect of changes in gravity on male germ cells remain poorly understood. The aim of this study was to determine the effect of simulated micro- and hypergravity on mouse sperm motility and the mechanisms of this change. For 1, 3 and 6 h, mouse sperm samples isolated from the caudal epididymis were subjected to simulated microgravity using a random position machine and 2g hypergravity using a centrifuge. The experimental samples were compared with static and dynamic controls. The sperm motility and the percentage of motile sperm were determined using microscopy and video analysis, cell respiration was determined by polarography, the protein content was assessed by Western blotting and the mRNA levels were determined using qRT-PCR. The results indicated that hypergravity conditions led to more significant changes than simulated microgravity conditions: after 1 h, the speed of sperm movement decreased, and after 3 h, the number of motile cells began to decrease. Under the microgravity model, the speed of movement did not change, but the motile spermatozoa decreased after 6 h of exposure. These changes are likely associated with a change in the structure of the microtubule cytoskeleton, and changes in the energy supply are an adaptive reaction to changes in sperm motility. MDPI 2020-07-17 /pmc/articles/PMC7404272/ /pubmed/32709012 http://dx.doi.org/10.3390/ijms21145054 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Ogneva, Irina V.
Usik, Maria A.
Biryukov, Nikolay S.
Zhdankina, Yuliya S.
Sperm Motility of Mice under Simulated Microgravity and Hypergravity
title Sperm Motility of Mice under Simulated Microgravity and Hypergravity
title_full Sperm Motility of Mice under Simulated Microgravity and Hypergravity
title_fullStr Sperm Motility of Mice under Simulated Microgravity and Hypergravity
title_full_unstemmed Sperm Motility of Mice under Simulated Microgravity and Hypergravity
title_short Sperm Motility of Mice under Simulated Microgravity and Hypergravity
title_sort sperm motility of mice under simulated microgravity and hypergravity
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404272/
https://www.ncbi.nlm.nih.gov/pubmed/32709012
http://dx.doi.org/10.3390/ijms21145054
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