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Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C

Hepatitis C virus (HCV) infections can cause permanent liver-related diseases, including hepatocellular carcinoma (HCC). Low mortality and incidence of HCC have been observed in patients with chronic hepatitis C undergoing direct-acting antiviral therapy. Tumor suppressive let-7 family members are d...

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Autores principales: Tsai, Yi-Shan, Yeh, Ming-Lun, Tsai, Pei-Chien, Huang, Ching-I, Huang, Chung-Feng, Hsieh, Meng-Hsuan, Liu, Ta-Wei, Lin, Yi-Hung, Liang, Po-Cheng, Lin, Zu-Yau, Chen, Shinn-Cherng, Huang, Jee-Fu, Chuang, Wan-Long, Dai, Chia-Yen, Yu, Ming-Lung
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404305/
https://www.ncbi.nlm.nih.gov/pubmed/32668728
http://dx.doi.org/10.3390/ijms21144945
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author Tsai, Yi-Shan
Yeh, Ming-Lun
Tsai, Pei-Chien
Huang, Ching-I
Huang, Chung-Feng
Hsieh, Meng-Hsuan
Liu, Ta-Wei
Lin, Yi-Hung
Liang, Po-Cheng
Lin, Zu-Yau
Chen, Shinn-Cherng
Huang, Jee-Fu
Chuang, Wan-Long
Dai, Chia-Yen
Yu, Ming-Lung
author_facet Tsai, Yi-Shan
Yeh, Ming-Lun
Tsai, Pei-Chien
Huang, Ching-I
Huang, Chung-Feng
Hsieh, Meng-Hsuan
Liu, Ta-Wei
Lin, Yi-Hung
Liang, Po-Cheng
Lin, Zu-Yau
Chen, Shinn-Cherng
Huang, Jee-Fu
Chuang, Wan-Long
Dai, Chia-Yen
Yu, Ming-Lung
author_sort Tsai, Yi-Shan
collection PubMed
description Hepatitis C virus (HCV) infections can cause permanent liver-related diseases, including hepatocellular carcinoma (HCC). Low mortality and incidence of HCC have been observed in patients with chronic hepatitis C undergoing direct-acting antiviral therapy. Tumor suppressive let-7 family members are down-regulated in HCC. The present study, therefore, aimed to investigate whether expression levels for the full spectrum of let-7 family members (let-7a, 7b, 7c, 7d, 7e, 7f, 7g, 7i, and miR-98) in the circulatory system are useful as surveillance biomarkers for liver-related diseases to monitor treatment efficacy during HCV infection. To this end, we measured the levels of mature circulating let-7 family members using quantitative reverse transcription-PCR in 236 patients with HCV infection, and 147 age- and sex-matched controls. Using hierarchical cluster analysis and principal component analysis, three clusters were obtained after measuring expression levels of let-7 family members in the patients and controls. Cluster 1 included let-7a/d/e/g, Cluster 2 comprised let-7b and let-7i, and Cluster 3 comprised let-7c/f/miR-98. Let-7b/c/g represented the three clusters and showed the best survival response to liver cancer when analyzed with respect to patient data. Therefore, considering the circulating levels of let7 b/c/g as representatives of the let-7 family may facilitate effective monitoring of liver-related disease.
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spelling pubmed-74043052020-08-18 Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C Tsai, Yi-Shan Yeh, Ming-Lun Tsai, Pei-Chien Huang, Ching-I Huang, Chung-Feng Hsieh, Meng-Hsuan Liu, Ta-Wei Lin, Yi-Hung Liang, Po-Cheng Lin, Zu-Yau Chen, Shinn-Cherng Huang, Jee-Fu Chuang, Wan-Long Dai, Chia-Yen Yu, Ming-Lung Int J Mol Sci Article Hepatitis C virus (HCV) infections can cause permanent liver-related diseases, including hepatocellular carcinoma (HCC). Low mortality and incidence of HCC have been observed in patients with chronic hepatitis C undergoing direct-acting antiviral therapy. Tumor suppressive let-7 family members are down-regulated in HCC. The present study, therefore, aimed to investigate whether expression levels for the full spectrum of let-7 family members (let-7a, 7b, 7c, 7d, 7e, 7f, 7g, 7i, and miR-98) in the circulatory system are useful as surveillance biomarkers for liver-related diseases to monitor treatment efficacy during HCV infection. To this end, we measured the levels of mature circulating let-7 family members using quantitative reverse transcription-PCR in 236 patients with HCV infection, and 147 age- and sex-matched controls. Using hierarchical cluster analysis and principal component analysis, three clusters were obtained after measuring expression levels of let-7 family members in the patients and controls. Cluster 1 included let-7a/d/e/g, Cluster 2 comprised let-7b and let-7i, and Cluster 3 comprised let-7c/f/miR-98. Let-7b/c/g represented the three clusters and showed the best survival response to liver cancer when analyzed with respect to patient data. Therefore, considering the circulating levels of let7 b/c/g as representatives of the let-7 family may facilitate effective monitoring of liver-related disease. MDPI 2020-07-13 /pmc/articles/PMC7404305/ /pubmed/32668728 http://dx.doi.org/10.3390/ijms21144945 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Tsai, Yi-Shan
Yeh, Ming-Lun
Tsai, Pei-Chien
Huang, Ching-I
Huang, Chung-Feng
Hsieh, Meng-Hsuan
Liu, Ta-Wei
Lin, Yi-Hung
Liang, Po-Cheng
Lin, Zu-Yau
Chen, Shinn-Cherng
Huang, Jee-Fu
Chuang, Wan-Long
Dai, Chia-Yen
Yu, Ming-Lung
Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C
title Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C
title_full Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C
title_fullStr Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C
title_full_unstemmed Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C
title_short Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C
title_sort clusters of circulating let-7 family tumor suppressors are associated with clinical characteristics of chronic hepatitis c
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404305/
https://www.ncbi.nlm.nih.gov/pubmed/32668728
http://dx.doi.org/10.3390/ijms21144945
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