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Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C
Hepatitis C virus (HCV) infections can cause permanent liver-related diseases, including hepatocellular carcinoma (HCC). Low mortality and incidence of HCC have been observed in patients with chronic hepatitis C undergoing direct-acting antiviral therapy. Tumor suppressive let-7 family members are d...
Autores principales: | , , , , , , , , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
MDPI
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404305/ https://www.ncbi.nlm.nih.gov/pubmed/32668728 http://dx.doi.org/10.3390/ijms21144945 |
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author | Tsai, Yi-Shan Yeh, Ming-Lun Tsai, Pei-Chien Huang, Ching-I Huang, Chung-Feng Hsieh, Meng-Hsuan Liu, Ta-Wei Lin, Yi-Hung Liang, Po-Cheng Lin, Zu-Yau Chen, Shinn-Cherng Huang, Jee-Fu Chuang, Wan-Long Dai, Chia-Yen Yu, Ming-Lung |
author_facet | Tsai, Yi-Shan Yeh, Ming-Lun Tsai, Pei-Chien Huang, Ching-I Huang, Chung-Feng Hsieh, Meng-Hsuan Liu, Ta-Wei Lin, Yi-Hung Liang, Po-Cheng Lin, Zu-Yau Chen, Shinn-Cherng Huang, Jee-Fu Chuang, Wan-Long Dai, Chia-Yen Yu, Ming-Lung |
author_sort | Tsai, Yi-Shan |
collection | PubMed |
description | Hepatitis C virus (HCV) infections can cause permanent liver-related diseases, including hepatocellular carcinoma (HCC). Low mortality and incidence of HCC have been observed in patients with chronic hepatitis C undergoing direct-acting antiviral therapy. Tumor suppressive let-7 family members are down-regulated in HCC. The present study, therefore, aimed to investigate whether expression levels for the full spectrum of let-7 family members (let-7a, 7b, 7c, 7d, 7e, 7f, 7g, 7i, and miR-98) in the circulatory system are useful as surveillance biomarkers for liver-related diseases to monitor treatment efficacy during HCV infection. To this end, we measured the levels of mature circulating let-7 family members using quantitative reverse transcription-PCR in 236 patients with HCV infection, and 147 age- and sex-matched controls. Using hierarchical cluster analysis and principal component analysis, three clusters were obtained after measuring expression levels of let-7 family members in the patients and controls. Cluster 1 included let-7a/d/e/g, Cluster 2 comprised let-7b and let-7i, and Cluster 3 comprised let-7c/f/miR-98. Let-7b/c/g represented the three clusters and showed the best survival response to liver cancer when analyzed with respect to patient data. Therefore, considering the circulating levels of let7 b/c/g as representatives of the let-7 family may facilitate effective monitoring of liver-related disease. |
format | Online Article Text |
id | pubmed-7404305 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | MDPI |
record_format | MEDLINE/PubMed |
spelling | pubmed-74043052020-08-18 Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C Tsai, Yi-Shan Yeh, Ming-Lun Tsai, Pei-Chien Huang, Ching-I Huang, Chung-Feng Hsieh, Meng-Hsuan Liu, Ta-Wei Lin, Yi-Hung Liang, Po-Cheng Lin, Zu-Yau Chen, Shinn-Cherng Huang, Jee-Fu Chuang, Wan-Long Dai, Chia-Yen Yu, Ming-Lung Int J Mol Sci Article Hepatitis C virus (HCV) infections can cause permanent liver-related diseases, including hepatocellular carcinoma (HCC). Low mortality and incidence of HCC have been observed in patients with chronic hepatitis C undergoing direct-acting antiviral therapy. Tumor suppressive let-7 family members are down-regulated in HCC. The present study, therefore, aimed to investigate whether expression levels for the full spectrum of let-7 family members (let-7a, 7b, 7c, 7d, 7e, 7f, 7g, 7i, and miR-98) in the circulatory system are useful as surveillance biomarkers for liver-related diseases to monitor treatment efficacy during HCV infection. To this end, we measured the levels of mature circulating let-7 family members using quantitative reverse transcription-PCR in 236 patients with HCV infection, and 147 age- and sex-matched controls. Using hierarchical cluster analysis and principal component analysis, three clusters were obtained after measuring expression levels of let-7 family members in the patients and controls. Cluster 1 included let-7a/d/e/g, Cluster 2 comprised let-7b and let-7i, and Cluster 3 comprised let-7c/f/miR-98. Let-7b/c/g represented the three clusters and showed the best survival response to liver cancer when analyzed with respect to patient data. Therefore, considering the circulating levels of let7 b/c/g as representatives of the let-7 family may facilitate effective monitoring of liver-related disease. MDPI 2020-07-13 /pmc/articles/PMC7404305/ /pubmed/32668728 http://dx.doi.org/10.3390/ijms21144945 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/). |
spellingShingle | Article Tsai, Yi-Shan Yeh, Ming-Lun Tsai, Pei-Chien Huang, Ching-I Huang, Chung-Feng Hsieh, Meng-Hsuan Liu, Ta-Wei Lin, Yi-Hung Liang, Po-Cheng Lin, Zu-Yau Chen, Shinn-Cherng Huang, Jee-Fu Chuang, Wan-Long Dai, Chia-Yen Yu, Ming-Lung Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C |
title | Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C |
title_full | Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C |
title_fullStr | Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C |
title_full_unstemmed | Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C |
title_short | Clusters of Circulating let-7 Family Tumor Suppressors Are Associated with Clinical Characteristics of Chronic Hepatitis C |
title_sort | clusters of circulating let-7 family tumor suppressors are associated with clinical characteristics of chronic hepatitis c |
topic | Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404305/ https://www.ncbi.nlm.nih.gov/pubmed/32668728 http://dx.doi.org/10.3390/ijms21144945 |
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