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Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation among Olea europaea Fruit Tissues in Late Development

Fruit ripening and abscission are the results of the cell wall modification concerning different components of the signaling network. However, molecular-genetic information on the cross-talk between ripe fruit and their abscission zone (AZ) remains limited. In this study, we investigated transcripti...

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Autores principales: Briegas, Beatriz, Corbacho, Jorge, Parra-Lobato, Maria C., Paredes, Miguel A., Labrador, Juana, Gallardo, Mercedes, Gomez-Jimenez, Maria C.
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404322/
https://www.ncbi.nlm.nih.gov/pubmed/32650402
http://dx.doi.org/10.3390/ijms21144819
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author Briegas, Beatriz
Corbacho, Jorge
Parra-Lobato, Maria C.
Paredes, Miguel A.
Labrador, Juana
Gallardo, Mercedes
Gomez-Jimenez, Maria C.
author_facet Briegas, Beatriz
Corbacho, Jorge
Parra-Lobato, Maria C.
Paredes, Miguel A.
Labrador, Juana
Gallardo, Mercedes
Gomez-Jimenez, Maria C.
author_sort Briegas, Beatriz
collection PubMed
description Fruit ripening and abscission are the results of the cell wall modification concerning different components of the signaling network. However, molecular-genetic information on the cross-talk between ripe fruit and their abscission zone (AZ) remains limited. In this study, we investigated transcriptional and hormonal changes in olive (Olea europaea L. cv Picual) pericarp and AZ tissues of fruit at the last stage of ripening, when fruit abscission occurs, to establish distinct tissue-specific expression patterns related to cell-wall modification, plant-hormone, and vesicle trafficking in combination with data on hormonal content. In this case, transcriptome profiling reveals that gene encoding members of the α-galactosidase and β-hexosaminidase families associated with up-regulation of RabB, RabD, and RabH classes of Rab-GTPases were exclusively transcribed in ripe fruit enriched in ABA, whereas genes of the arabinogalactan protein, laccase, lyase, endo-β-mannanase, ramnose synthase, and xyloglucan endotransglucosylase/hydrolase families associated with up-regulation of RabC, RabE, and RabG classes of Rab-GTPases were exclusively transcribed in AZ-enriched mainly in JA, which provide the first insights into the functional divergences among these protein families. The enrichment of these protein families in different tissues in combination with data on transcript abundance offer a tenable set of key genes of the regulatory network between olive fruit tissues in late development.
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spelling pubmed-74043222020-08-18 Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation among Olea europaea Fruit Tissues in Late Development Briegas, Beatriz Corbacho, Jorge Parra-Lobato, Maria C. Paredes, Miguel A. Labrador, Juana Gallardo, Mercedes Gomez-Jimenez, Maria C. Int J Mol Sci Article Fruit ripening and abscission are the results of the cell wall modification concerning different components of the signaling network. However, molecular-genetic information on the cross-talk between ripe fruit and their abscission zone (AZ) remains limited. In this study, we investigated transcriptional and hormonal changes in olive (Olea europaea L. cv Picual) pericarp and AZ tissues of fruit at the last stage of ripening, when fruit abscission occurs, to establish distinct tissue-specific expression patterns related to cell-wall modification, plant-hormone, and vesicle trafficking in combination with data on hormonal content. In this case, transcriptome profiling reveals that gene encoding members of the α-galactosidase and β-hexosaminidase families associated with up-regulation of RabB, RabD, and RabH classes of Rab-GTPases were exclusively transcribed in ripe fruit enriched in ABA, whereas genes of the arabinogalactan protein, laccase, lyase, endo-β-mannanase, ramnose synthase, and xyloglucan endotransglucosylase/hydrolase families associated with up-regulation of RabC, RabE, and RabG classes of Rab-GTPases were exclusively transcribed in AZ-enriched mainly in JA, which provide the first insights into the functional divergences among these protein families. The enrichment of these protein families in different tissues in combination with data on transcript abundance offer a tenable set of key genes of the regulatory network between olive fruit tissues in late development. MDPI 2020-07-08 /pmc/articles/PMC7404322/ /pubmed/32650402 http://dx.doi.org/10.3390/ijms21144819 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Briegas, Beatriz
Corbacho, Jorge
Parra-Lobato, Maria C.
Paredes, Miguel A.
Labrador, Juana
Gallardo, Mercedes
Gomez-Jimenez, Maria C.
Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation among Olea europaea Fruit Tissues in Late Development
title Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation among Olea europaea Fruit Tissues in Late Development
title_full Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation among Olea europaea Fruit Tissues in Late Development
title_fullStr Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation among Olea europaea Fruit Tissues in Late Development
title_full_unstemmed Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation among Olea europaea Fruit Tissues in Late Development
title_short Transcriptome and Hormone Analyses Revealed Insights into Hormonal and Vesicle Trafficking Regulation among Olea europaea Fruit Tissues in Late Development
title_sort transcriptome and hormone analyses revealed insights into hormonal and vesicle trafficking regulation among olea europaea fruit tissues in late development
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404322/
https://www.ncbi.nlm.nih.gov/pubmed/32650402
http://dx.doi.org/10.3390/ijms21144819
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