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Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors
Enzyme-powered motors self-propel through the catalysis of in situ bioavailable fuels, which makes them excellent candidates for biomedical applications. However, fundamental issues like their motion in biological fluids and the understanding of the propulsion mechanism are critical aspects to be ta...
Autores principales: | , , , , , , , |
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Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
AAAS
2020
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Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404610/ https://www.ncbi.nlm.nih.gov/pubmed/32803169 http://dx.doi.org/10.34133/2020/2424972 |
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author | Arqué, Xavier Andrés, Xavier Mestre, Rafael Ciraulo, Bernard Ortega Arroyo, Jaime Quidant, Romain Patiño, Tania Sánchez, Samuel |
author_facet | Arqué, Xavier Andrés, Xavier Mestre, Rafael Ciraulo, Bernard Ortega Arroyo, Jaime Quidant, Romain Patiño, Tania Sánchez, Samuel |
author_sort | Arqué, Xavier |
collection | PubMed |
description | Enzyme-powered motors self-propel through the catalysis of in situ bioavailable fuels, which makes them excellent candidates for biomedical applications. However, fundamental issues like their motion in biological fluids and the understanding of the propulsion mechanism are critical aspects to be tackled before a future application in biomedicine. Herein, we investigated the physicochemical effects of ionic species on the self-propulsion of urease-powered micromotors. Results showed that the presence of PBS, NaOH, NaCl, and HEPES reduced self-propulsion of urease-powered micromotors pointing towards ion-dependent mechanisms of motion. We studied the 3D motion of urease micromotors using digital holographic microscopy to rule out any motor-surface interaction as the cause of motion decay when salts are present in the media. In order to protect and minimize the negative effect of ionic species on micromotors' performance, we coated the motors with methoxypolyethylene glycol amine (mPEG) showing higher speed compared to noncoated motors at intermediate ionic concentrations. These results provide new insights into the mechanism of urease-powered micromotors, study the effect of ionic media, and contribute with potential solutions to mitigate the reduction of mobility of enzyme-powered micromotors. |
format | Online Article Text |
id | pubmed-7404610 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | AAAS |
record_format | MEDLINE/PubMed |
spelling | pubmed-74046102020-08-13 Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors Arqué, Xavier Andrés, Xavier Mestre, Rafael Ciraulo, Bernard Ortega Arroyo, Jaime Quidant, Romain Patiño, Tania Sánchez, Samuel Research (Wash D C) Research Article Enzyme-powered motors self-propel through the catalysis of in situ bioavailable fuels, which makes them excellent candidates for biomedical applications. However, fundamental issues like their motion in biological fluids and the understanding of the propulsion mechanism are critical aspects to be tackled before a future application in biomedicine. Herein, we investigated the physicochemical effects of ionic species on the self-propulsion of urease-powered micromotors. Results showed that the presence of PBS, NaOH, NaCl, and HEPES reduced self-propulsion of urease-powered micromotors pointing towards ion-dependent mechanisms of motion. We studied the 3D motion of urease micromotors using digital holographic microscopy to rule out any motor-surface interaction as the cause of motion decay when salts are present in the media. In order to protect and minimize the negative effect of ionic species on micromotors' performance, we coated the motors with methoxypolyethylene glycol amine (mPEG) showing higher speed compared to noncoated motors at intermediate ionic concentrations. These results provide new insights into the mechanism of urease-powered micromotors, study the effect of ionic media, and contribute with potential solutions to mitigate the reduction of mobility of enzyme-powered micromotors. AAAS 2020-07-27 /pmc/articles/PMC7404610/ /pubmed/32803169 http://dx.doi.org/10.34133/2020/2424972 Text en Copyright © 2020 Xavier Arqué et al. https://creativecommons.org/licenses/by/4.0/ Exclusive Licensee Science and Technology Review Publishing House. Distributed under a Creative Commons Attribution License (CC BY 4.0). |
spellingShingle | Research Article Arqué, Xavier Andrés, Xavier Mestre, Rafael Ciraulo, Bernard Ortega Arroyo, Jaime Quidant, Romain Patiño, Tania Sánchez, Samuel Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors |
title | Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors |
title_full | Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors |
title_fullStr | Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors |
title_full_unstemmed | Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors |
title_short | Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors |
title_sort | ionic species affect the self-propulsion of urease-powered micromotors |
topic | Research Article |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404610/ https://www.ncbi.nlm.nih.gov/pubmed/32803169 http://dx.doi.org/10.34133/2020/2424972 |
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