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Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors

Enzyme-powered motors self-propel through the catalysis of in situ bioavailable fuels, which makes them excellent candidates for biomedical applications. However, fundamental issues like their motion in biological fluids and the understanding of the propulsion mechanism are critical aspects to be ta...

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Autores principales: Arqué, Xavier, Andrés, Xavier, Mestre, Rafael, Ciraulo, Bernard, Ortega Arroyo, Jaime, Quidant, Romain, Patiño, Tania, Sánchez, Samuel
Formato: Online Artículo Texto
Lenguaje:English
Publicado: AAAS 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404610/
https://www.ncbi.nlm.nih.gov/pubmed/32803169
http://dx.doi.org/10.34133/2020/2424972
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author Arqué, Xavier
Andrés, Xavier
Mestre, Rafael
Ciraulo, Bernard
Ortega Arroyo, Jaime
Quidant, Romain
Patiño, Tania
Sánchez, Samuel
author_facet Arqué, Xavier
Andrés, Xavier
Mestre, Rafael
Ciraulo, Bernard
Ortega Arroyo, Jaime
Quidant, Romain
Patiño, Tania
Sánchez, Samuel
author_sort Arqué, Xavier
collection PubMed
description Enzyme-powered motors self-propel through the catalysis of in situ bioavailable fuels, which makes them excellent candidates for biomedical applications. However, fundamental issues like their motion in biological fluids and the understanding of the propulsion mechanism are critical aspects to be tackled before a future application in biomedicine. Herein, we investigated the physicochemical effects of ionic species on the self-propulsion of urease-powered micromotors. Results showed that the presence of PBS, NaOH, NaCl, and HEPES reduced self-propulsion of urease-powered micromotors pointing towards ion-dependent mechanisms of motion. We studied the 3D motion of urease micromotors using digital holographic microscopy to rule out any motor-surface interaction as the cause of motion decay when salts are present in the media. In order to protect and minimize the negative effect of ionic species on micromotors' performance, we coated the motors with methoxypolyethylene glycol amine (mPEG) showing higher speed compared to noncoated motors at intermediate ionic concentrations. These results provide new insights into the mechanism of urease-powered micromotors, study the effect of ionic media, and contribute with potential solutions to mitigate the reduction of mobility of enzyme-powered micromotors.
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spelling pubmed-74046102020-08-13 Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors Arqué, Xavier Andrés, Xavier Mestre, Rafael Ciraulo, Bernard Ortega Arroyo, Jaime Quidant, Romain Patiño, Tania Sánchez, Samuel Research (Wash D C) Research Article Enzyme-powered motors self-propel through the catalysis of in situ bioavailable fuels, which makes them excellent candidates for biomedical applications. However, fundamental issues like their motion in biological fluids and the understanding of the propulsion mechanism are critical aspects to be tackled before a future application in biomedicine. Herein, we investigated the physicochemical effects of ionic species on the self-propulsion of urease-powered micromotors. Results showed that the presence of PBS, NaOH, NaCl, and HEPES reduced self-propulsion of urease-powered micromotors pointing towards ion-dependent mechanisms of motion. We studied the 3D motion of urease micromotors using digital holographic microscopy to rule out any motor-surface interaction as the cause of motion decay when salts are present in the media. In order to protect and minimize the negative effect of ionic species on micromotors' performance, we coated the motors with methoxypolyethylene glycol amine (mPEG) showing higher speed compared to noncoated motors at intermediate ionic concentrations. These results provide new insights into the mechanism of urease-powered micromotors, study the effect of ionic media, and contribute with potential solutions to mitigate the reduction of mobility of enzyme-powered micromotors. AAAS 2020-07-27 /pmc/articles/PMC7404610/ /pubmed/32803169 http://dx.doi.org/10.34133/2020/2424972 Text en Copyright © 2020 Xavier Arqué et al. https://creativecommons.org/licenses/by/4.0/ Exclusive Licensee Science and Technology Review Publishing House. Distributed under a Creative Commons Attribution License (CC BY 4.0).
spellingShingle Research Article
Arqué, Xavier
Andrés, Xavier
Mestre, Rafael
Ciraulo, Bernard
Ortega Arroyo, Jaime
Quidant, Romain
Patiño, Tania
Sánchez, Samuel
Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors
title Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors
title_full Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors
title_fullStr Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors
title_full_unstemmed Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors
title_short Ionic Species Affect the Self-Propulsion of Urease-Powered Micromotors
title_sort ionic species affect the self-propulsion of urease-powered micromotors
topic Research Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7404610/
https://www.ncbi.nlm.nih.gov/pubmed/32803169
http://dx.doi.org/10.34133/2020/2424972
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