Cargando…

Novel Ergot Alkaloids Production from Penicillium citrinum Employing Response Surface Methodology Technique

Ergot alkaloids are novel pharmaceutical and therapeutic agents synthesized in this study using fungal species Penicillium citrinum. To get the maximum yield of ergot alkaloids a statistical process of response surface methodology was employed using surface culture fermentation technique. Initially,...

Descripción completa

Detalles Bibliográficos
Autores principales: Shahid, Memuna Ghafoor, Nadeem, Muhammad, Gulzar, Ahmed, Saleem, Muhammad, Rehman, Hafeez ur, Ghafoor, Gul Zareen, Hayyat, Muhammad Umar, Shahzad, Laila, Arif, Rabia, Nelofer, Rubina
Formato: Online Artículo Texto
Lenguaje:English
Publicado: MDPI 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7405006/
https://www.ncbi.nlm.nih.gov/pubmed/32610508
http://dx.doi.org/10.3390/toxins12070427
_version_ 1783567209676668928
author Shahid, Memuna Ghafoor
Nadeem, Muhammad
Gulzar, Ahmed
Saleem, Muhammad
Rehman, Hafeez ur
Ghafoor, Gul Zareen
Hayyat, Muhammad Umar
Shahzad, Laila
Arif, Rabia
Nelofer, Rubina
author_facet Shahid, Memuna Ghafoor
Nadeem, Muhammad
Gulzar, Ahmed
Saleem, Muhammad
Rehman, Hafeez ur
Ghafoor, Gul Zareen
Hayyat, Muhammad Umar
Shahzad, Laila
Arif, Rabia
Nelofer, Rubina
author_sort Shahid, Memuna Ghafoor
collection PubMed
description Ergot alkaloids are novel pharmaceutical and therapeutic agents synthesized in this study using fungal species Penicillium citrinum. To get the maximum yield of ergot alkaloids a statistical process of response surface methodology was employed using surface culture fermentation technique. Initially, the strain of Penicillium was improved using physical (ultraviolet (UV) and chemical (ethyl methane sulfonate (EMS) treatments to get the maximum yield of ergot alkaloids through surface culture fermentation technique. After improving the strain, survival rate of colonies of Penicillium citrinum treated with UV and EMS was observed. Only 2.04% living colonies were observed after 150 min of exposure of Penicillium citrinum in UV light and 3.2% living colonies were observed after 20 min of the exposure in EMS. The mutated strains of Penicillium citrinum were screened for their production of ergot alkaloids and after fermentation experiments, maximum yield was obtained from PCUV-4 and PCEMS-1 strains. After strain improvement, Plackett–Burman design (PBD) and Box–Behnken design (BBD) of RSM were employed and 10-fold yield enhancement (35.60 mg/100 mL) of ergot alkaloids was achieved. This enhancement in yield of ergot alkaloids proved the positive impacts of RSM and UV on the yield of ergot alkaloids. The study provides a cost effective, economical and sustainable process to produce medically important ergot alkaloids which can be used in various pharmaceutical formulations to treat human diseases.
format Online
Article
Text
id pubmed-7405006
institution National Center for Biotechnology Information
language English
publishDate 2020
publisher MDPI
record_format MEDLINE/PubMed
spelling pubmed-74050062020-08-17 Novel Ergot Alkaloids Production from Penicillium citrinum Employing Response Surface Methodology Technique Shahid, Memuna Ghafoor Nadeem, Muhammad Gulzar, Ahmed Saleem, Muhammad Rehman, Hafeez ur Ghafoor, Gul Zareen Hayyat, Muhammad Umar Shahzad, Laila Arif, Rabia Nelofer, Rubina Toxins (Basel) Article Ergot alkaloids are novel pharmaceutical and therapeutic agents synthesized in this study using fungal species Penicillium citrinum. To get the maximum yield of ergot alkaloids a statistical process of response surface methodology was employed using surface culture fermentation technique. Initially, the strain of Penicillium was improved using physical (ultraviolet (UV) and chemical (ethyl methane sulfonate (EMS) treatments to get the maximum yield of ergot alkaloids through surface culture fermentation technique. After improving the strain, survival rate of colonies of Penicillium citrinum treated with UV and EMS was observed. Only 2.04% living colonies were observed after 150 min of exposure of Penicillium citrinum in UV light and 3.2% living colonies were observed after 20 min of the exposure in EMS. The mutated strains of Penicillium citrinum were screened for their production of ergot alkaloids and after fermentation experiments, maximum yield was obtained from PCUV-4 and PCEMS-1 strains. After strain improvement, Plackett–Burman design (PBD) and Box–Behnken design (BBD) of RSM were employed and 10-fold yield enhancement (35.60 mg/100 mL) of ergot alkaloids was achieved. This enhancement in yield of ergot alkaloids proved the positive impacts of RSM and UV on the yield of ergot alkaloids. The study provides a cost effective, economical and sustainable process to produce medically important ergot alkaloids which can be used in various pharmaceutical formulations to treat human diseases. MDPI 2020-06-29 /pmc/articles/PMC7405006/ /pubmed/32610508 http://dx.doi.org/10.3390/toxins12070427 Text en © 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).
spellingShingle Article
Shahid, Memuna Ghafoor
Nadeem, Muhammad
Gulzar, Ahmed
Saleem, Muhammad
Rehman, Hafeez ur
Ghafoor, Gul Zareen
Hayyat, Muhammad Umar
Shahzad, Laila
Arif, Rabia
Nelofer, Rubina
Novel Ergot Alkaloids Production from Penicillium citrinum Employing Response Surface Methodology Technique
title Novel Ergot Alkaloids Production from Penicillium citrinum Employing Response Surface Methodology Technique
title_full Novel Ergot Alkaloids Production from Penicillium citrinum Employing Response Surface Methodology Technique
title_fullStr Novel Ergot Alkaloids Production from Penicillium citrinum Employing Response Surface Methodology Technique
title_full_unstemmed Novel Ergot Alkaloids Production from Penicillium citrinum Employing Response Surface Methodology Technique
title_short Novel Ergot Alkaloids Production from Penicillium citrinum Employing Response Surface Methodology Technique
title_sort novel ergot alkaloids production from penicillium citrinum employing response surface methodology technique
topic Article
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7405006/
https://www.ncbi.nlm.nih.gov/pubmed/32610508
http://dx.doi.org/10.3390/toxins12070427
work_keys_str_mv AT shahidmemunaghafoor novelergotalkaloidsproductionfrompenicilliumcitrinumemployingresponsesurfacemethodologytechnique
AT nadeemmuhammad novelergotalkaloidsproductionfrompenicilliumcitrinumemployingresponsesurfacemethodologytechnique
AT gulzarahmed novelergotalkaloidsproductionfrompenicilliumcitrinumemployingresponsesurfacemethodologytechnique
AT saleemmuhammad novelergotalkaloidsproductionfrompenicilliumcitrinumemployingresponsesurfacemethodologytechnique
AT rehmanhafeezur novelergotalkaloidsproductionfrompenicilliumcitrinumemployingresponsesurfacemethodologytechnique
AT ghafoorgulzareen novelergotalkaloidsproductionfrompenicilliumcitrinumemployingresponsesurfacemethodologytechnique
AT hayyatmuhammadumar novelergotalkaloidsproductionfrompenicilliumcitrinumemployingresponsesurfacemethodologytechnique
AT shahzadlaila novelergotalkaloidsproductionfrompenicilliumcitrinumemployingresponsesurfacemethodologytechnique
AT arifrabia novelergotalkaloidsproductionfrompenicilliumcitrinumemployingresponsesurfacemethodologytechnique
AT neloferrubina novelergotalkaloidsproductionfrompenicilliumcitrinumemployingresponsesurfacemethodologytechnique