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Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)

Detection of free gastric cancer cells in peritoneal lavages and ascites plays a vital role in gastric cancer. However, due to the low content of cancer cells in patients’ peritoneal lavages, traditional detection methods lack sensitivity and cannot satisfy clinical demand. In this study, we used an...

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Autores principales: Zhang, Yuzhao, Zhao, Junhua, Yu, Haibo, Li, Pan, Liang, Wenfeng, Liu, Zhu, Lee, Gwo-Bin, Liu, Lianqing, Li, Wen Jung, Wang, Zhenning
Formato: Online Artículo Texto
Lenguaje:English
Publicado: American Association for the Advancement of Science 2020
Materias:
Acceso en línea:https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406364/
https://www.ncbi.nlm.nih.gov/pubmed/32821829
http://dx.doi.org/10.1126/sciadv.aba9628
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author Zhang, Yuzhao
Zhao, Junhua
Yu, Haibo
Li, Pan
Liang, Wenfeng
Liu, Zhu
Lee, Gwo-Bin
Liu, Lianqing
Li, Wen Jung
Wang, Zhenning
author_facet Zhang, Yuzhao
Zhao, Junhua
Yu, Haibo
Li, Pan
Liang, Wenfeng
Liu, Zhu
Lee, Gwo-Bin
Liu, Lianqing
Li, Wen Jung
Wang, Zhenning
author_sort Zhang, Yuzhao
collection PubMed
description Detection of free gastric cancer cells in peritoneal lavages and ascites plays a vital role in gastric cancer. However, due to the low content of cancer cells in patients’ peritoneal lavages, traditional detection methods lack sensitivity and cannot satisfy clinical demand. In this study, we used an optically induced electrokinetics (OEK) microfluidic method for label-free separation and characterization of patient gastric cancer cells. This method showed high effectiveness and sensitivity. We successfully separated cancer cells from a simulated peritoneal lavage mixture of gastric cancer cell lines and peritoneal lavage cells in a ratio of 1:1000. We further separated gastric cancer cells from six patients’ ascites with purity up to 71%. In addition, we measured the cell membrane capacitances, which may be used as a biomarker for gastric cancer cells. Thus, our method can be used to effectively and rapidly detect peritoneal metastasis and to acquire cellular electrical information.
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spelling pubmed-74063642020-08-19 Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK) Zhang, Yuzhao Zhao, Junhua Yu, Haibo Li, Pan Liang, Wenfeng Liu, Zhu Lee, Gwo-Bin Liu, Lianqing Li, Wen Jung Wang, Zhenning Sci Adv Research Articles Detection of free gastric cancer cells in peritoneal lavages and ascites plays a vital role in gastric cancer. However, due to the low content of cancer cells in patients’ peritoneal lavages, traditional detection methods lack sensitivity and cannot satisfy clinical demand. In this study, we used an optically induced electrokinetics (OEK) microfluidic method for label-free separation and characterization of patient gastric cancer cells. This method showed high effectiveness and sensitivity. We successfully separated cancer cells from a simulated peritoneal lavage mixture of gastric cancer cell lines and peritoneal lavage cells in a ratio of 1:1000. We further separated gastric cancer cells from six patients’ ascites with purity up to 71%. In addition, we measured the cell membrane capacitances, which may be used as a biomarker for gastric cancer cells. Thus, our method can be used to effectively and rapidly detect peritoneal metastasis and to acquire cellular electrical information. American Association for the Advancement of Science 2020-08-05 /pmc/articles/PMC7406364/ /pubmed/32821829 http://dx.doi.org/10.1126/sciadv.aba9628 Text en Copyright © 2020 The Authors, some rights reserved; exclusive licensee American Association for the Advancement of Science. No claim to original U.S. Government Works. Distributed under a Creative Commons Attribution NonCommercial License 4.0 (CC BY-NC). https://creativecommons.org/licenses/by-nc/4.0/ https://creativecommons.org/licenses/by-nc/4.0/This is an open-access article distributed under the terms of the Creative Commons Attribution-NonCommercial license (https://creativecommons.org/licenses/by-nc/4.0/) , which permits use, distribution, and reproduction in any medium, so long as the resultant use is not for commercial advantage and provided the original work is properly cited.
spellingShingle Research Articles
Zhang, Yuzhao
Zhao, Junhua
Yu, Haibo
Li, Pan
Liang, Wenfeng
Liu, Zhu
Lee, Gwo-Bin
Liu, Lianqing
Li, Wen Jung
Wang, Zhenning
Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)
title Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)
title_full Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)
title_fullStr Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)
title_full_unstemmed Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)
title_short Detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (OEK)
title_sort detection and isolation of free cancer cells from ascites and peritoneal lavages using optically induced electrokinetics (oek)
topic Research Articles
url https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406364/
https://www.ncbi.nlm.nih.gov/pubmed/32821829
http://dx.doi.org/10.1126/sciadv.aba9628
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