Cargando…
In vivo PET Imaging of Gliogenesis After Cerebral Ischemia in Rats
In vivo positron emission tomography of neuroinflammation has mainly focused on the evaluation of glial cell activation using radiolabeled ligands. However, the non-invasive imaging of neuroinflammatory cell proliferation has been scarcely evaluated so far. In vivo and ex vivo assessment of gliogene...
Autores principales: | , , , , , , , , , , , |
---|---|
Formato: | Online Artículo Texto |
Lenguaje: | English |
Publicado: |
Frontiers Media S.A.
2020
|
Materias: | |
Acceso en línea: | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406641/ https://www.ncbi.nlm.nih.gov/pubmed/32848565 http://dx.doi.org/10.3389/fnins.2020.00793 |
_version_ | 1783567463899725824 |
---|---|
author | Ardaya, María Joya, Ana Padro, Daniel Plaza-García, Sandra Gómez-Vallejo, Vanessa Sánchez, Mercedes Garbizu, Maider Cossío, Unai Matute, Carlos Cavaliere, Fabio Llop, Jordi Martín, Abraham |
author_facet | Ardaya, María Joya, Ana Padro, Daniel Plaza-García, Sandra Gómez-Vallejo, Vanessa Sánchez, Mercedes Garbizu, Maider Cossío, Unai Matute, Carlos Cavaliere, Fabio Llop, Jordi Martín, Abraham |
author_sort | Ardaya, María |
collection | PubMed |
description | In vivo positron emission tomography of neuroinflammation has mainly focused on the evaluation of glial cell activation using radiolabeled ligands. However, the non-invasive imaging of neuroinflammatory cell proliferation has been scarcely evaluated so far. In vivo and ex vivo assessment of gliogenesis after transient middle cerebral artery occlusion (MCAO) in rats was carried out using PET imaging with the marker of cell proliferation 3′-Deoxy-3′-[18F] fluorothymidine ([(18)F]FLT), magnetic resonance imaging (MRI) and fluorescence immunohistochemistry. MRI-T(2)W studies showed the presence of the brain infarction at 24 h after MCAO affecting cerebral cortex and striatum. In vivo PET imaging showed a significant increase in [(18)F]FLT uptake in the ischemic territory at day 7 followed by a progressive decline from day 14 to day 28 after ischemia onset. In addition, immunohistochemistry studies using Ki67, CD11b, and GFAP to evaluate proliferation of microglia and astrocytes confirmed the PET findings showing the increase of glial proliferation at day 7 after ischemia followed by decrease later on. Hence, these results show that [(18)F]FLT provides accurate quantitative information on the time course of glial proliferation in experimental stroke. Finally, this novel brain imaging method might guide on the imaging evaluation of the role of gliogenesis after stroke. |
format | Online Article Text |
id | pubmed-7406641 |
institution | National Center for Biotechnology Information |
language | English |
publishDate | 2020 |
publisher | Frontiers Media S.A. |
record_format | MEDLINE/PubMed |
spelling | pubmed-74066412020-08-25 In vivo PET Imaging of Gliogenesis After Cerebral Ischemia in Rats Ardaya, María Joya, Ana Padro, Daniel Plaza-García, Sandra Gómez-Vallejo, Vanessa Sánchez, Mercedes Garbizu, Maider Cossío, Unai Matute, Carlos Cavaliere, Fabio Llop, Jordi Martín, Abraham Front Neurosci Neuroscience In vivo positron emission tomography of neuroinflammation has mainly focused on the evaluation of glial cell activation using radiolabeled ligands. However, the non-invasive imaging of neuroinflammatory cell proliferation has been scarcely evaluated so far. In vivo and ex vivo assessment of gliogenesis after transient middle cerebral artery occlusion (MCAO) in rats was carried out using PET imaging with the marker of cell proliferation 3′-Deoxy-3′-[18F] fluorothymidine ([(18)F]FLT), magnetic resonance imaging (MRI) and fluorescence immunohistochemistry. MRI-T(2)W studies showed the presence of the brain infarction at 24 h after MCAO affecting cerebral cortex and striatum. In vivo PET imaging showed a significant increase in [(18)F]FLT uptake in the ischemic territory at day 7 followed by a progressive decline from day 14 to day 28 after ischemia onset. In addition, immunohistochemistry studies using Ki67, CD11b, and GFAP to evaluate proliferation of microglia and astrocytes confirmed the PET findings showing the increase of glial proliferation at day 7 after ischemia followed by decrease later on. Hence, these results show that [(18)F]FLT provides accurate quantitative information on the time course of glial proliferation in experimental stroke. Finally, this novel brain imaging method might guide on the imaging evaluation of the role of gliogenesis after stroke. Frontiers Media S.A. 2020-07-30 /pmc/articles/PMC7406641/ /pubmed/32848565 http://dx.doi.org/10.3389/fnins.2020.00793 Text en Copyright © 2020 Ardaya, Joya, Padro, Plaza-García, Gómez-Vallejo, Sánchez, Garbizu, Cossío, Matute, Cavaliere, Llop and Martín. http://creativecommons.org/licenses/by/4.0/ This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms. |
spellingShingle | Neuroscience Ardaya, María Joya, Ana Padro, Daniel Plaza-García, Sandra Gómez-Vallejo, Vanessa Sánchez, Mercedes Garbizu, Maider Cossío, Unai Matute, Carlos Cavaliere, Fabio Llop, Jordi Martín, Abraham In vivo PET Imaging of Gliogenesis After Cerebral Ischemia in Rats |
title | In vivo PET Imaging of Gliogenesis After Cerebral Ischemia in Rats |
title_full | In vivo PET Imaging of Gliogenesis After Cerebral Ischemia in Rats |
title_fullStr | In vivo PET Imaging of Gliogenesis After Cerebral Ischemia in Rats |
title_full_unstemmed | In vivo PET Imaging of Gliogenesis After Cerebral Ischemia in Rats |
title_short | In vivo PET Imaging of Gliogenesis After Cerebral Ischemia in Rats |
title_sort | in vivo pet imaging of gliogenesis after cerebral ischemia in rats |
topic | Neuroscience |
url | https://www.ncbi.nlm.nih.gov/pmc/articles/PMC7406641/ https://www.ncbi.nlm.nih.gov/pubmed/32848565 http://dx.doi.org/10.3389/fnins.2020.00793 |
work_keys_str_mv | AT ardayamaria invivopetimagingofgliogenesisaftercerebralischemiainrats AT joyaana invivopetimagingofgliogenesisaftercerebralischemiainrats AT padrodaniel invivopetimagingofgliogenesisaftercerebralischemiainrats AT plazagarciasandra invivopetimagingofgliogenesisaftercerebralischemiainrats AT gomezvallejovanessa invivopetimagingofgliogenesisaftercerebralischemiainrats AT sanchezmercedes invivopetimagingofgliogenesisaftercerebralischemiainrats AT garbizumaider invivopetimagingofgliogenesisaftercerebralischemiainrats AT cossiounai invivopetimagingofgliogenesisaftercerebralischemiainrats AT matutecarlos invivopetimagingofgliogenesisaftercerebralischemiainrats AT cavalierefabio invivopetimagingofgliogenesisaftercerebralischemiainrats AT llopjordi invivopetimagingofgliogenesisaftercerebralischemiainrats AT martinabraham invivopetimagingofgliogenesisaftercerebralischemiainrats |